Chinese Bulletin of Botany ›› 2005, Vol. 22 ›› Issue (增刊): 43-49.

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Plant Regeneration through Somatic Embryogenesis Formation from Cotyledons of Camellia oleifera Clone

1ZHANG Zhi-Jun 2LUO Shu-Ping 2LI Ya-Ling 3BI Fang-Cheng 3TAN Xiao-Feng   

    1. 1 (College of Life Science, Nanjing University, Nanjing 210093) 
    2. 2 (College of Agronomy, Xinjiang Agricultural University, Urmq 830052) 
    3. 3 (College of Life Science, Centre-south Forestry University, Changsha 412006)
  • Received:2003-11-12 Revised:2004-09-20 Online:2005-08-10 Published:2005-08-10

Abstract: Cotyledons of explants from ‘xianglin-4’ (XL-4), a Camellia oleifera clone, were cultured in MS medium with different hormones. Results showed that the optimal medium for embryogenetic callus formation was MS with 2.0 mg.L-1 2,4-D and 1.0 mg.L-1 KT; the optimal medium for shoot development via embryos was MS with 2.5 mg.L-1 6-BA and 1.5 mg.L-1 IAA; the optimal medium for root inducement was MS with 7.0 mg.L-1 NAA. Some materials from different processes of plantlet regeneration were discriminated by RAPD analysis. However, no mutations or differentiation were discovered. Therefore, it can be concluded that plantlets from tissue culture do not differ from quondam clones and can maintain the hereditary consistency well.

Key words: Camellia oleifera, Clone, Somatic embryogenesis, Tissue culture