Cloning, Bioinformatics Analysis of LOC110717159 Gene from Chenopodium Quinoa and Overexpression in Transgenic Arabidopsis

doi:10.11983/CBB25040

Abstract

Abstract: INTRODUCTION: The glycoside hydrolase (GH) gene family plays a crucial role in regulating plant grain size.

RATIONALE: To elucidate its biological role during grain filling, we cloning and functionally characterized LOC110717159, a GH gene in quinoa, through bioinformatics analysis, spatiotemporal expression profiling, and heterologous expression experiments in Arabidopsis.

RESULTS: LOC110717159 has a 1 023 bp coding sequence (encoding 339 amino acids) in both germplasms, differing by a single nucleotide. Analysis of the ~ 2 000 bp LOC110717159 promoter identified 119 cis-elements, including three hormone-responsive elements (MeJA, SA, and GA). Phylogenetically, it is closely related to Beta vulgaris, Spinacia oleracea and Amaranthus tricolor. qRT-PCR revealed high expression of LOC110717159 in large-grained quinoa at 21 days after anthesis (21 DAA). Arabidopsis overexpression lines showed significantly reduced 1 000-grain weight (P < 0.05) but no change in grain length and width. Haplotype analysis of 17 quinoa germplasms indicated that 62.5% of large germplasms (≥ 4.0 g/1 000 grains) carried the large-grained haplotype, while 77.8% of small germplasms (≤ 3.0 g) shared the small-grained haplotype.

CONCLUSION: In conclusion, LOC110717159 likely functions as a negative regulator in quinoa grain filling.

T3-generation transgenic Arabidopsis lines and seed morphological characteristics. (A)–(C) Wild-type plants; (D)–(F) AtOE1、AtOE2、AtOE3; ns: No significant difference; *P ≤ 0.05

Tao Xiaosuo, Yao Xiaohua, Yao Youhua. Cloning, Bioinformatics Analysis of LOC110717159 Gene from Chenopodium Quinoa and Overexpression in Transgenic Arabidopsis[J]. Chinese Bulletin of Botany, DOI: 10.11983/CBB25040.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB25040

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