Cloning and Characterization of an AP2/EREBP Gene TmAP2-1 from Tetraena mongolica

doi:10.3724/SP.J.1259.2013.00023

Abstract

Abstract: AP2/EREBP transcription factors play important roles in plant morphology, development, and stress responses. We cloned and characterized a putative AP2/ERF domain-containing gene from Tetraena mongolica by homology-based cloning and rapid amplification of cDNA ends. This gene, designated TmAP2-1 (accession no. JQ676996), encodes a protein of 483 amino acids with 2 copies of AP2 DNA binding domain. Subcellular localization analysis revealed that TmAP2-1 protein localized in the nucleus. TmAP2-1 protein has no transactivation activity in yeast. The expression of TmAP2-1 is induced by cold, salt, polyethylene glycol and abscisic acid. TmAP2-1 is detected in roots, shoots and leaves of T. mongolica, with the highest expression in leaves. Overexpression of TmAP2-1, accumulation of more soluble sugars in T. mongolica callus, but in reduced lipid content. TmAP2-1 probably represents an AP2/EREBP gene that plays a regulatory role in tolerance of T. mongolica to abiotic stresses by affecting accumulation of soluble sugars.

Key words: abiotic stress, AP2/EREBP, gene cloning, soluble sugars, Tetraena mongolica

CLC Number:

Q781

Yujing Wang, Minchun Li, Wang Wu, Hanying Wu, Yinong Xu. Cloning and Characterization of an AP2/EREBP Gene TmAP2-1 from Tetraena mongolica[J]. Chinese Bulletin of Botany, 2013, 48(1): 23-33.

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URL: https://www.chinbullbotany.com/EN/10.3724/SP.J.1259.2013.00023

https://www.chinbullbotany.com/EN/Y2013/V48/I1/23

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