Chinese Bulletin of Botany ›› 2024, Vol. 59 ›› Issue (1): 22-33.DOI: 10.11983/CBB23025

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

Bioinformatic and Expression Pattern Analysis of dfr-miR160a and Target Gene DfARF10 in Dryopteris fragrans

Zhaoxuan Zhong1, Dongrui Zhang1, Lu Li1, Ying Su2, Daining Wang1, Zeran Wang1, Yang Liu1, Ying Chang1,*()   

  1. 1College of Life Science, Northeast Agricultural University, Haerbin 150036, China
    2Nanshan Affiliated School of Beijing Normal University, Shenzhen 518000, China
  • Received:2023-02-27 Accepted:2023-08-29 Online:2024-01-01 Published:2023-09-07
  • Contact: *E-mail:

Abstract: To further understand the molecular mechanism underlying miRNA regulation of growth and development of Dryopteris fragrans, we screened the differentially expressed dfr-pri-mir160a through the miRNA database established earlier in the laboratory, and predicted its target gene as DfARF10. The target relationship between dfr-pri-mir160a and DfARF10 was verified by tobacco transient co-transformation, together with double luciferase (LUC) activity. The results showed that the GUS and LUC activity in tobacco leaves co-injected with dfr-pri-mir160a and DfARF10 decreased significantly. qRT-PCR analysis showed that dfr-miR160a and its target gene DfARF10 were expressed in the gametophytes, roots, petioles, leaves and sporangium of D. fragrans, with the highest expression in the leaves and the lowest in the roots. We analyzed the effects of drought, NaCl, high temperature and low temperature stress treatments on dfr-miR160a and its target gene DfARF10 through qRT-PCR. Under drought and high temperature treatment, the relative expression of dfr-miR160a was up-regulated, but under NaCl treatment, the expression of dfr-miR160a was down-regulated. Under low temperature treatment, the expression of dfr-miR160a was down-regulated at 0-1 h and was up-regulated at 3-48 h. The expression of DfARF10 was up-regulated under NaCl, high temperature and low temperature treatments. However, under drought treatment, the expression of DfARF10 decreased, distinct from dfr-miR160a. The above results indicated that target gene of dfr-miR160 was DfARF10, and both of them can respond to abiotic stress treatment. This study provides a new scientific basis for revealing the abiotic stress resistance mechanism of D. fragrans at the molecular level.

Key words: Dryopteris fragrans, dfr-miR160a, DfARF10, abiotic stress