Expression Analysis of B-class MADS-box Genes from Catalpa speciosa

doi:10.11983/CBB15053

Chinese Bulletin of Botany ›› 2016, Vol. 51 ›› Issue (2): 210-217.DOI: 10.11983/CBB15053

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Expression Analysis of B-class MADS-box Genes from Catalpa speciosa

Danlong Jing, Yan Xia, Shougong Zhang, Junhui Wang^*

Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, China Forest Genetic Resource, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China

Received:2015-03-12 Revised:2015-07-05 Online:2016-03-01 Published:2016-03-31
Contact: Junhui Wang

Abstract

Abstract: The cDNA sequence of B-class MADS-box genes CaspAP3 and CaspPI involved in flower development were isolated from the flower bud of Catalpa speciosa by homology cloning. The open reading frame (ORF) of CaspAP3 was 696 bp, encoding 231 amino acids, and the ORF of CaspPI was 639 bp, encoding 212 amino acids. Molecular phylogeny analysis and protein sequence alignment suggested that CaspAP3 groups with the AP3/DEF lineages and the C domain of CaspAP3 contained two conserved motifs, euAP3 and PI-derived motifs. However, CaspPI grouped with the PI/GLO lineages, and the C domain of CaspPI contained a conserved PI motif. CaspAP3 and CaspPI mRNA expression was mainly concentrated in petals and stamens but occurred at the primordium to mature stages of petals and stamens during morphological differentiation of organs. The expression of both factors peaked earlier in stamens than in petals, and the peak expression for both in petals appeared at the stage of rapid elongation. Meanwhile, the time of expression was the same with morphological differentiation of petals and stamens.

Danlong Jing, Yan Xia, Shougong Zhang, Junhui Wang. Expression Analysis of B-class MADS-box Genes from Catalpa speciosa[J]. Chinese Bulletin of Botany, 2016, 51(2): 210-217.

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Expression Analysis of B-class MADS-box Genes from Catalpa speciosa

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