Rice Blast Resistance of Wild Rice in Yunnan

doi:10.11983/CBB17112

Abstract

Abstract: Wild species of Oryza contain numerous genes of economic importance and are being used as an important cultivated rice gene pool to expand the genetic background, improve yield and quality, and increase the ability for resistance or tolerance to pests as well as bioic and abiotic stresses. O. meyeriana is one of three native wild rice species in China, mainly distributed in Yunnan province. To further understand the disease resistance to rice blast, we performed a systematic investigation of rice blast resistance with O. meyeriana. O. meyeriana plants were inoculated by injection with multiple blast strains from different rice planting areas. Then, we cloned and functionally identified Pid2 and Pid3 orthologous genes in O. meyeriana. O. meyeriana was sensitive to all blast isolates used in this experiment. The DNA and amino acid sequences significantly differed between Pid2 and its orthologs in O. meyeriana, as did Pid3 and its orthologs. Nipponbare plants overexpressing the orthologous genes of Pid2 and Pid3 showed mildly enhanced susceptibility to Magnaporthe oryzae. The orthologs of blast-resistant genes in O. meyeriana may be susceptible alleles. O. meyeriana exhibited resistance to rice blast under natural inoculation likely due to a xeromorphic leaf structure that reduces susceptibility to M. oryzae. Characterization of these genes will help breed elite rice. Our study provides new information and ideas for research and utilization of O. meyeriana.

Key words: wild rice (Oryza meyeriana), rice blast resistance, R gene, gene cloning, Magnaporthe oryzae

Wang Yunqian, Su Yanhong, Yang Rui, Li Xin, Li Jing, Zeng Qianchun, Luo Qiong. Rice Blast Resistance of Wild Rice in Yunnan[J]. Chinese Bulletin of Botany, 2018, 53(4): 477-486.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB17112

https://www.chinbullbotany.com/EN/Y2018/V53/I4/477

Figures/Tables 4

Figure 1 Identification of blast resistance in Oryza meyeriana(A) Injection inoculation; (B) Spray inoculation; (C) Inoculated by blast strains ZB15 and Zhong-10-8-14, respectively

Figure 2 Amino acid sequence alignment of OmPid2 and orthologs in riceOmPid2: Oryza meyeriana; R-Pid2: Digu; S-pid2: Nipponbare. Signal peptide domain (21-52 amino acids), B-lectin domain (68-184 amino acids), PAN domain (355-436 amino acids) and Serine/threonine kinase (STK) domain (519-789 amino acids) are underlined. Arrow indicates the amino acid that distinguishes resistant and susceptible alleles of rice blast resistance gene Pid2.

Figure 3 Amino acid sequence alignment of OmPid3 and orthologs in riceOmPid3: Oryza meyeriana; R-Pid3: Digu; S-pid3: Nipponbare. Nucleotide-binding site (NBS) domain (158-445 amino acids) and Leucine-rich repeat (LRR) domain (563-923 amino acids) are underlined.

Figure 4 Characterisation of transgenic rice linesMolecular detection of transgenic rice lines with Hygromycin primer, OmPid2 gene-specific primer (A) and OmPid3 gene-specific primer (B); Blast resistance idetification of OmPid2 (C) and OmPid3 (D) overexpressing transgenic rice lines inoculated by blast strains; Overexpressing transgenic rice lines OmPid2 and OmPid3 (E) were inoculated by blast strains ZB15 and Zhong-10-8-14, respectively. HYG: Hygromycin; Di: The resistant rice variety Digu; N: Wild-type susceptible rice variety Nipponbare

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