植物学报 ›› 2021, Vol. 56 ›› Issue (4): 451-461.DOI: 10.11983/CBB20205

• 技术方法 • 上一篇    下一篇

郁金樱愈伤组织诱导及植株再生

罗钱, 张燕莎, 欧静*()   

  1. 贵州大学林学院, 贵阳 550025
  • 收稿日期:2020-12-19 接受日期:2021-05-07 出版日期:2021-07-01 发布日期:2021-06-30
  • 通讯作者: 欧静
  • 作者简介:*E-mail: coloroj@126.com
  • 基金资助:
    贵州省农业科技支撑项目(20182287)

Callus Induction and Plant Regeneration of Cerasus serrulata var. lannesiana cv. ‘Grandiflora’

Qian Luo, Yansha Zhang, Jing Ou*()   

  1. College of Forestry, Guizhou University, Guiyang 550025, China
  • Received:2020-12-19 Accepted:2021-05-07 Online:2021-07-01 Published:2021-06-30
  • Contact: Jing Ou

摘要: 为建立郁金樱(Cerasus serrulata var. lannesiana cv. ‘Grandiflora’)再生体系, 以多年生母株小叶、一年生嫁接苗小叶、腋芽诱导小叶和增殖一代小叶为外植体, 探讨不同外植体和植物激素组合对郁金樱愈伤组织诱导、不定芽分化、增殖和生根的影响。结果表明, 4种外植体均可诱导出愈伤组织, 除多年生母株小叶外皆分化出不定芽, 外植体幼化程度越高, 后期培养潜力越大。以增殖一代小叶为外植体效果最佳, 其最适愈伤组织诱导培养基为MS+0.5 mg·L-16-BA+1.0 mg·L-12,4-D, 诱导率达96.22%; 最佳分化培养基为MS+1.0 mg·L-16-BA+0.1 mg·L-12,4-D+0.1 mg·L-1TDZ, 分化率达78.14%; 最佳增殖培养基为MS+1.0 mg·L-16-BA, 增殖系数可达7.85; 最佳生根培养基为不含任何激素的1/2MS培养基, 生根率达100%。不同外植体获得的再生植株移栽成活后生长差异显著, 以增殖一代小叶诱导的再生植株长势最佳。

关键词: 郁金樱, 愈伤组织, 外植体, 植株再生, 组织培养

Abstract: In order to establish the regeneration system of Cerasus serrulata var. lannesiana cv. ‘Grandiflora’, the effects of different explants and plant hormone combinations on callus induction, adventitious bud differentiation, proliferation and rooting were studied using perennial mother plant leaflets, annual grafted seedling leaflets, axillary bud induction leaflets and proliferative first-generation leaflets as explants. The results showed that callus could be induced from all four explants, and adventitious buds could be derived from all explants except the leaflets of perennial mother plants. The higher the degree of explants’ juvenility, the greater the success of subsequent culture, with the best explants were the proliferative first generation leaflets. The best medium for callus induction was MS+0.5 mg·L-1 6-BA+1.0 mg·L-1 2,4-D, and the induction rate was 96.22%. The optimal medium for differentiation was MS+1.0 mg·L-1 6-BA+0.1 mg·L-1 2,4-D+0.1 mg·L-1 TDZ, and the differentiation rate was 78.14%. The optimal medium for proliferation was MS+1.0 mg·L-1 6-BA, and the proliferation coefficient reached 7.85. The optimal medium for rooting was 1/2MS without any hormone, and the regenerated plants with 100% rooting rate were obtained. The regenerated plants from different explants grew very differently, and those induced from first generation of proliferative leaflets had the best growth.

Key words: Cerasus serrulata var. lannesiana cv. ‘Grandiflora’, callus, explants, plant regeneration, tissue culture