西印度醋栗组培快繁技术初探

doi:10.11983/CBB24113

植物学报 ›› 2025, Vol. 60 ›› Issue (4): 611-620.DOI: 10.11983/CBB24113 cstr: 32102.14.CBB24113

西印度醋栗组培快繁技术初探

李彤¹, 李楚然¹, 张芷瑜¹, 付晓熳¹, 刘云¹, 张颖君²^,^*(), 杨力颖³, 赵平¹^,^*()

¹西南林业大学西南地区林业生物质资源高效利用国家林业和草原局重点实验室, 昆明 650224
²中国科学院昆明植物研究所植物化学与天然药物重点实验室, 昆明 650201
³云南新兴绿化工程有限公司, 昆明 650228

收稿日期:2024-07-23 接受日期:2024-12-26 出版日期:2025-07-10 发布日期:2024-12-27
通讯作者: 张颖君,赵平
基金资助:
国家自然科学基金(32270426);国家自然科学基金(82074124)

A Preliminary Study on Tissue Culture and Rapid Propagation Technology of Phyllanthus acidus

Tong Li¹, Churan Li¹, Zhiyu Zhang¹, Xiaoman Fu¹, Yun Liu¹, Yingjun Zhang²^,^*(), Liying Yang³, Ping Zhao¹^,^*()

¹Key Laboratory of State Forestry and Grassland Administration on Highly-Efficient Utilization of Forestry Biomass Resources in Southwest China, Southwest Forestry University, Kunming 650224, China
²Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
³Yunnan Xinxing Greening Engineering Co., Ltd., Kunming 650228, China

Received:2024-07-23 Accepted:2024-12-26 Online:2025-07-10 Published:2024-12-27
Contact: Yingjun Zhang, Ping Zhao

摘要/Abstract

摘要： 西印度醋栗(Phyllanthus acidus)是一种生长于热带地区的乔木, 兼具食用和药用价值, 开展其组培快繁技术研究, 可促进其资源的持续开发与利用。以西印度醋栗茎尖为外植体, 对初代培养、继代增殖培养及生根培养方案进行初步筛选。结果表明, 初代培养最适培养基为MS+2.0 mg·L^-1 6-BA+0.2 mg·L^-1 NAA, 外植体诱导萌发率达81.11%; 继代增殖最适培养基为MS+1.0 mg·L^-1 6-BA+0.2 mg·L^-1 IBA, 增殖系数达1.86; 最适生根培养基为MS+1.5 mg·L^-1 IBA, 生根率达83.00%; 炼苗7天后移栽, 移栽基质为珍珠岩:泥炭土:腐殖质=1:1:1 (v/v/v), 成活率为90%。从接种外植体到获得再生植株约90天。研究结果为西印度醋栗的资源保护与产业化应用提供了技术支持。

关键词: 西印度醋栗, 组织培养, 快速繁殖

Abstract: INTRODUCTION A preliminary tissue culture system for Phyllanthus acidus was established. RATIONALE In this study, the stem tips of P. acidus were used as explants, and the schemes of primary culture, secondary proliferation culture and rooting culture were screened.RESULTS The results showed that the optimal medium for primary culture was MS+2.0 mg·L^-1 6-BA+0.2 mg·L^-1 NAA, and the induced germination rate of explants reached 81.11%. The optimal medium for subculture was MS+1.0 mg·L^-1 6-BA+0.2 mg·L^-1 IBA, and the proliferation coefficient was 1.86. The optimal medium for rooting was MS+1.5 mg·L^-1 IBA, and the rooting rate reached 83.00%. After 7 days of cultivation, the plantlets were transplanted with perlite, peat soil and humus=1:1:1 (v/v/v), and the survival rate was 90%.CONCLUSION The most suitable medium for primary culture using the stem tip of P. acidus as explant was MS medium and the phytohormones were 2.0 mg·L^-1 6-BA and 0.2 mg·L^-1 NAA; the optimal phytohormones for subculture were 1.0 mg·L^-1 6-BA and 0.2 mg·L^-1 IBA; the optimal phytohormone for rooting culture was 1.5 mg·L^-1 IBA.

Key words: Phyllanthus acidus, tissue culture, rapid propagation

李彤, 李楚然, 张芷瑜, 付晓熳, 刘云, 张颖君, 杨力颖, 赵平. 西印度醋栗组培快繁技术初探. 植物学报, 2025, 60(4): 611-620.

Tong Li, Churan Li, Zhiyu Zhang, Xiaoman Fu, Yun Liu, Yingjun Zhang, Liying Yang, Ping Zhao. A Preliminary Study on Tissue Culture and Rapid Propagation Technology of Phyllanthus acidus. Chinese Bulletin of Botany, 2025, 60(4): 611-620.

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https://www.chinbullbotany.com/CN/Y2025/V60/I4/611

图/表 8

表1 不同植物生长调节剂配比对西印度醋栗腋芽诱导的影响

Table 1 Effects of different proportions of plant growth regulators on axillary bud induction of Phyllanthus acidus

Treatments	6-BA (mg·L^-1)	NAA (mg·L^-1)	Number of explants	Adventitious bud induction rate (%)	Growth condition
1	0.5	0.1	90	27.78±1.11 b	++
2	0.5	0.2	90	22.22±4.01 c	++
3	0.5	0.3	90	20.00±1.92 c	+
4	1.0	0.1	90	53.33±7.70 ab	++
5	1.0	0.2	90	62.22±4.00 a	++
6	1.0	0.3	90	70.00±15.28 a	+
7	2.0	0.1	90	66.67±6.67 a	++
8	2.0	0.2	90	76.67±14.53 a	+++
9	2.0	0.3	90	75.56±11.60 a	+

图1 不同植物生长调节剂配比对西印度醋栗腋芽诱导的影响 (A) 西印度醋栗植株; (B)-(J) 在不同质量浓度6-BA与NAA配比的培养基中初代培养25天后组培苗的生长状态(B-J中6-BA与NAA浓度配比同表1)。Bars=1 cm

Figure 1 Effects of different plant growth regulator ratios on axillary bud induction of Phyllanthus acidus (A) P. acidus plant; (B)-(D) The growth status of tissue cultured plantlets after 25 days of initial cultivation in different mass concentration ratios of 6-BA and NAA (concentration ratios of 6-BA and NAA in B-J are the same as shown in Table 1). Bars=1 cm

表2 不同基本培养基对西印度醋栗腋芽诱导的影响

Table 2 Effects of different basic media on axillary bud induction of Phyllanthus acidus

Treatments	Culture medium	6-BA (mg·L^-1)	NAA (mg·L^-1)	Number of explants	Adventitious bud induction rate (%)	Growth condition
1	MS	2.0	0.2	90	81.11±2.22 a	+++
2	1/2 MS	2.0	0.2	90	58.89±2.94 b	+
3	1/3 MS	2.0	0.2	90	52.00±1.17 b	+

图2 不同基本培养基对西印度醋栗腋芽诱导的影响 (A) 外植体在MS培养基中初代培养25天后组培苗的生长状态; (B) 外植体在1/2 MS培养基中初代培养25天后组培苗的生长状态; (C) 外植体在1/3 MS培养基中初代培养25天后组培苗的生长状态。Bars=1 cm

Figure 2 Effects of different basic media on axillary bud induction of Phyllanthus acidus (A) The growth status of tissue cultured plantlets after 25 days of initial cultivation of explants in MS medium; (B) The growth status of tissue cultured plantlets after 25 days of initial cultivation of explants in 1/2 MS medium; (C) The growth status of tissue cultured plantlets after 25 days of initial cultivation of explants in 1/3 MS medium. Bars=1 cm

表3 不同植物生长调节剂配比对西印度醋栗继代与增殖的影响

Table 3 Effects of different proportions of plant growth regulators on subgeneration and proliferation of Phyllanthus acidus

Treatments	6-BA (mg·L^-1)	IBA (mg·L^-1)	Number of explants	Number of buds after proliferation	Multiplication factor	Plant height (cm)	Growth condition
1	1.0	0.1	90	159±0.58 a	1.77±0.13 a	2.71±0.06 b	++
2	1.0	0.2	90	168±1.53 a	1.86±0.20 a	3.34±0.07 a	+++
3	1.0	0.3	90	104±0.33 b	1.16±0.01 b	2.00±0.06 c	+

图3 不同植物生长调节剂配比对西印度醋栗继代与增殖的影响 (A) 外植体在1.0 mg·L-1 6-BA+0.1 mg·L-1 IBA培养基中继代增殖25天后组培苗的生长状态; (B) 外植体在1.0 mg·L-1 6-BA+0.2 mg·L-1 IBA培养基中继代增殖25天后组培苗的生长状态; (C) 外植体在1.0 mg·L-1 6-BA+0.3 mg·L-1 IBA培养基中继代增殖25天后组培苗的生长状态。Bars=1 cm

Figure 3 Effects of different proportions of plant growth regulators on subgeneration and proliferation of Phyllanthus acidus (A) The growth status of tissue cultured plantlets after 25 days of subculture proliferation in 1.0 mg·L-1 6-BA+0.1 mg·L-1 IBA medium; (B) The growth status of tissue cultured plantlets after 25 days of subculture proliferation in 1.0 mg·L-1 6-BA+0.2 mg·L-1 IBA medium; (C) The growth status of tissue cultured plantlets after 25 days of subculture proliferation in 1.0 mg·L-1 6-BA+0.3 mg·L-1 IBA medium. Bars=1 cm

表4 不同植物生长调节剂配比对西印度醋栗生根的影响

Table 4 Effects of different proportions of plant growth regulators on rooting of Phyllanthus acidus

Treatments	NAA (mg·L^-1)	IBA (mg·L^-1)	Number of explants	Rooting rate (%)	Rooting number (n)	Root length (cm)	Growth condition
1	0.5	0	30	60.00±11.55 ab	8.40±0.70 a	0.99±0.06 d	++
2	1.0	0	30	66.67±6.67 a	9.00±0.84 a	1.02±0.05 d	++
3	1.5	0	30	33.33±8.82 b	1.50±0.22 d	0.91±0.06 d	+
4	0	0.5	30	76.67±8.82 a	2.70±0.37 d	1.87±0.08 c	++
5	0	1.0	30	70.00±15.28 a	4.80±4.80 c	2.70±0.15 b	+++
6	0	1.5	30	83.33±6.67 a	6.70±0.70 b	3.70±0.15 a	+++

图4 西印度醋栗组培苗的生根与移栽 (A)-(F) 外植体在不同浓度NAA和IBA配比的培养基中生根培养30天后组培苗地下部的生长状态(A-F中NAA与IBA浓度配比同表4); (G)-(L) 外植体在不同浓度NAA和IBA配比的培养基中生根培养30天后组培苗地上部的生长状态(G-L中NAA与IBA浓度配比同表4); (M) 移栽至珍珠岩:泥炭土:腐殖质=1:1:1 (v/v/v)的基质土, 生长30天后的情况。Bars=1 cm

Figure 4 Rooting and transplanting of Phyllanthus acidus plantlet (A)-(F) The growth status of the explants in the lower part of the tissue culture plot after rooting and culture in the medium with different concentrations of NAA and IBA (concentration ratios of NAA and IBA in A-F are the same as shown in Table 4); (G)-(L) Growth status of the explants in the upper part of the tissue culture site after rooting and culture in the medium with different concentrations of NAA and IBA (concentration ratios of NAA and IBA in G-L are the same as shown in Table 4); (M) Transplanting matrix soil with a volume ratio of perlite, peat soil and humus of 1:1:1 (v/v/v), growth after 30 days. Bars=1 cm

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西印度醋栗组培快繁技术初探

A Preliminary Study on Tissue Culture and Rapid Propagation Technology of Phyllanthus acidus

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