白檀离体快繁技术

doi:10.11983/CBB18034

植物学报 ›› 2018, Vol. 53 ›› Issue (5): 693-699.DOI: 10.11983/CBB18034

白檀离体快繁技术

安佰义¹, 郭才南¹, 包文慧¹, 李凤飞¹, 赵赫¹, 陈丽¹, 安丰云^2,^*()

¹吉林农业大学园艺学院, 长春 130118
²延边林业科学研究院, 延吉 133000

收稿日期:2018-02-28 接受日期:2018-06-22 出版日期:2018-09-01 发布日期:2018-07-04
通讯作者: 安丰云
作者简介:
† 共同第一作者。
基金资助:
长春市科技局项目(No.17DY014)、吉林省教育厅项目(No.JJKH20180665KJ)和吉林省科技厅重点科技攻关项目(No.20150- 204045NY)

Rapid Propagation of Symplocos paniculata In Vitro

An Baiyi¹, Guo Cainan¹, Bao Wenhui¹, Li Fengfei¹, Zhao He¹, Chen Li¹, An Fengyun^2,^*()

¹College of Horticulture, Jilin Agricultural University, Changchun 130118, China
²Yanbian Academy of Forestry, Yanji 133000, China

Received:2018-02-28 Accepted:2018-06-22 Online:2018-09-01 Published:2018-07-04
Contact: An Fengyun
About author:
† These authors contributed equally to this paper

摘要/Abstract

摘要： 以白檀(Symplocos paniculata)幼嫩茎段为实验材料, 通过对启动培养、增殖、生根培养及移栽的影响因子进行研究, 初步建立了白檀的组织培养体系。结果表明: 白檀外植体最适灭菌方案为0.1%升汞3分钟, 无菌苗获得率达81%; 最适初代启动培养基为1/2MS+30 g∙L^-1蔗糖+8 g∙L^-1琼脂, 出芽率达86.83%; 增殖最适培养基为1/2MS+1.0 mg∙L^-1 6-BA+0.02 mg∙L^-1 IBA+30 g∙L^-1蔗糖+8 g∙L^-1琼脂, 增殖系数达3.57; 最适生根培养基为WPM+0.5 mg∙L^-1 IBA+0.5 mg∙L^-1 NAA+20 g∙L^-1蔗糖+2 g∙L^-1 AC+8 g∙L^-1琼脂, 生根率达93%; 炼苗后, 移入园土:草炭土=1:1 (v/v)的基质中, 成活率达83%。

关键词: 白檀, 外植体, 组织培养

Abstract: With tender stem segments of Symplocos paniculata as the experiment material, the tissue culture system of S. paniculata was preliminarily established to research the key factors influencing initiation culture, proliferation, rooting culture and transplantation. Treatment with 0.1% HgCl₂ for 3 min was the optimum scheme for explant sterilization of S. paniculata; survival rate of sterile seedlings was 81%. The rate of germination of initiation culture was 86.83% in the medium with 1/2MS+30 g∙L^-1 sucrose+8 g∙L^-1 agar. The proliferation coefficient was 3.57 in optimum culture medium with 1/2MS+1.0 mg∙L^-1 6-BA+0.02 mg∙L^-1 IBA+30 g∙L^-1 sucrose+8 g∙L^-1 agar. The suitable rooting medium was WPM+0.5 mg∙L^-1 IBA+0.5 mg∙L^-1 NAA+20 g∙L^-1 sucrose+2 g∙L^-1 AC+8 g∙L^-1agar, and rooting rate reached 93%. After being acclimatized, the regenerated plants were transplanted into substrate of garden soil and peat soil (v:v=1:1), with survival rate of regenerated plants of 83%.

Key words: Symplocos paniculata, explant, tissue culture

安佰义, 郭才南, 包文慧, 李凤飞, 赵赫, 陈丽, 安丰云. 白檀离体快繁技术. 植物学报, 2018, 53(5): 693-699.

An Baiyi, Guo Cainan, Bao Wenhui, Li Fengfei, Zhao He, Chen Li, An Fengyun. Rapid Propagation of Symplocos paniculata In Vitro. Chinese Bulletin of Botany, 2018, 53(5): 693-699.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB18034

https://www.chinbullbotany.com/CN/Y2018/V53/I5/693

图/表 6

参考文献 17

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Sterilization time (min)	1	2	3	4	5
Acquisition rate (%)	46.0	70.0	81.0	45.0	10.8
Pollution rate (%)	50.0	25.0	18.2	16.7	14.0

Sterilization time (min)	1	2	3	4	5
Acquisition rate (%)	46.0	70.0	81.0	45.0	10.8
Pollution rate (%)	50.0	25.0	18.2	16.7	14.0

Culture medium	Average germination rate (%)	State of growth
WPM	43.30±5.03 eE	Sprouts grew generally, leaves were normal shape and color
Read	56.37±4.05 dD	Sprouts grew generally, leaves were normal shape but yellow
MS	14.13±5.00 fF	Sprouts grew poorly, leaves were narrow, curly, green
mMS	16.00±5.00 fF	Sprouts grew poorly, leaves were curly, green
1/2MS	86.83±5.53 aA	Sprouts grew well, leaves were flat and dark green
1/2mMS	54.33±4.51 dD	Sprouts grew well, leaves were curly, dark green
1/4MS	74.13±3.61 bB	Sprouts grew generally, leaves were curly, green
1/8MS	66.33±3.21 cC	Sprouts grew generally, leaves were curly and yellow

Culture medium	Average germination rate (%)	State of growth
WPM	43.30±5.03 eE	Sprouts grew generally, leaves were normal shape and color
Read	56.37±4.05 dD	Sprouts grew generally, leaves were normal shape but yellow
MS	14.13±5.00 fF	Sprouts grew poorly, leaves were narrow, curly, green
mMS	16.00±5.00 fF	Sprouts grew poorly, leaves were curly, green
1/2MS	86.83±5.53 aA	Sprouts grew well, leaves were flat and dark green
1/2mMS	54.33±4.51 dD	Sprouts grew well, leaves were curly, dark green
1/4MS	74.13±3.61 bB	Sprouts grew generally, leaves were curly, green
1/8MS	66.33±3.21 cC	Sprouts grew generally, leaves were curly and yellow

6-BA (mg∙L^-1)	IBA (mg∙L^-1)	Average proliferation coefficient	State of growth
0.06	0	1.33±0.21 kJ	Sprouted within 15 days in average, base of seedlings had few calli, lateral buds were weak
0.06	0.02	1.40±0.00 kIJ	Sprouted within 15 days in average, base of seedlings had very few calli, lateral buds were weak
0.06	0.05	1.47±0.15 kIJ	Sprouted within 15 days in average, base of seedlings had very few calli, lateral buds were weak
0.06	0.08	1.67±0.21 ijkHIJ	Sprouted within 16 days in average, base of seedlings had many calli, lateral buds were weak
0.3	0	1.63±0.23 jkHIJ	Sprouted within 15 days in average, base of seedlings had few calli, lateral buds were slender
0.3	0.02	1.57±0.12 jkHIJ	Sprouted within 22 days in average, base of seedlings had few calli, lateral buds were thick
0.3	0.05	1.57±0.29 jkHIJ	Sprouted within 15 days in average, base of seedlings had few calli, lateral buds were thick
0.3	0.08	1.90±0.20 hijGHI	Sprouted within 15 days in average, base of seedlings had very few calli, lateral buds were thick
0.3	0.2	2.43±0.12 fgDEF	Sprouted within 9 days in average, base of seedlings had few calli, lateral buds were brawny
0.3	0.5	1.70±0.17 ijkHIJ	Sprouted within 15 days in average, base of seedlings had many calli, lateral buds were thick
0.6	0	2.77±0.25 defCD	Sprouted within 13 days in average, base of seedlings had few calli, lateral buds were slender
0.6	0.02	2.03±0.06 hiFGH	Sprouted within 45 days in average, base of seedlings had many calli, lateral buds were thick
0.6	0.05	3.50±0.26 abAB	Sprouted within 13 days in average, base of seedlings had many calli, lateral buds were thick
0.6	0.08	2.93±0.15 cdCD	Sprouted within 16 days in average, base of seedlings had many calli, lateral buds were thick
0.6	0.2	2.60±0.20 defDE	Sprouted within 15 days in average, base of seedlings had very few calli, lateral buds were thick
0.6	0.5	3.17±0.29 bcBC	Sprouted within 15 days in average, base of seedlings had few calli, lateral buds were brawny
0.6	0.8	2.20±0.27 ghEFG	Sprouted within 13 days in average, base of seedlings had many calli, lateral buds were brawny
1.0	0	2.70±0.20 defCD	Sprouted within 12 days in average, base of seedlings had many calli, lateral buds were brawny
1.0	0.02	3.57±0.32 aAB	Sprouted within 5 days in average, base of seedlings had few calli, lateral buds were brawny
1.0	0.05	1.67±0.12 ijkHIJ	Sprouted within 5 days in average, base of seedlings had few calli, lateral buds were brawny
1.0	0.08	3.50±0.20 abAB	Sprouted within 14 days in average, base of seedlings had few calli, lateral buds were brawny
1.0	0.2	2.53±0.25 defgDE	Sprouted within 8 days in average, base of seedlings had very few calli, lateral buds were brawny
1.0	0.5	2.90±0.17 cdeCD	Sprouted within 9 days in average, base of seedlings had few calli, lateral buds were brawny
1.0	0.8	3.87±0.06 aA	Sprouted within 5 days in average, base of seedlings had few calli, lateral buds were brawny
1.0	1.1	2.93±0.32 cdCD	Sprouted within 4 days in average, base of seedlings had a great many calli, lateral buds were brawny

白檀离体快繁技术

Rapid Propagation of Symplocos paniculata In Vitro

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IBA (mg∙L^-1)	NAA (mg∙L^-1)	Average rooting rate (%)	State of roots growth
0	0	2.86±0.60 hH	Rooted within 50 days in average, the number of taproots were 1-2, root length reached 1-2 cm
0.02	0.02	10.01±0.10 gG	Rooted within 41 days in average, the number of taproots were 1-2, root length reached 1-4 cm
0.05	0.05	25.13±0.23 fF	Rooted within 35 days in average, the number of taproots were 1-2, root length reached 1-3 cm
0.08	0.08	33.17±0.15 eE	Rooted within 25 days in average, the number of taproots were 3-4, root length reached 1.5-3 cm
0.2	0.2	88.00±1.73 dD	Rooted within 21 days in average, the number of taproots were 3-4, root length reached 1.5-3 cm
0.5	0.5	93.00±0.00 aA	Rooted within 21 days in average, the number of taproots were 6-7, root length reached 2.5-3 cm
0.8	0.8	92.03±0.06 bB	Rooted within 38 days in average, the number of taproots were 5-6, root length reached 1-2 cm
1.5	1.5	90.17±0.15 cC	Rooted within 60 days in average, the number of taproots were 3-4, root length reached 1-2 cm

Cultivation medium	Transplant number (individual plants)	Surviving number (individual plants)	Survival rate (%)
Garden soil:sand=1:1 (v/v)	30	23	77
Garden soil:peat soil=1:1 (v/v)	30	25	83
Peat soil:sand=1:1 (v/v)	30	21	70

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