植物类胡萝卜素提取与分析技术研究进展

doi:10.11983/CBB18091

植物学报 ›› 2018, Vol. 53 ›› Issue (5): 700-709.DOI: 10.11983/CBB18091

植物类胡萝卜素提取与分析技术研究进展

何卿^1,², 孙国峰², 林秦文², 李晓东^2,^*(), 张金政^2,^*()

¹国家知识产权局专利局专利审查协作北京中心, 北京 100160
²中国科学院植物研究所, 北京 100093

收稿日期:2018-04-09 接受日期:2018-07-16 出版日期:2018-09-01 发布日期:2018-11-29
通讯作者: 李晓东,张金政
作者简介:
† 共同第一作者。
基金资助:
科技基础性工作专项子课题(No.2015FY210100-10)

Advances in Extraction and Analysis Technologies of Plant Carotenoids

He Qing^1,², Sun Guofeng², Lin Qinwen², Li Xiaodong^2,^*(), Zhang Jinzheng^2,^*()

¹Patent Examination Collaborating Beijing Center of the Patent Office, China National Intellectual Property Administration, Beijing 100160, China
²Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China

Received:2018-04-09 Accepted:2018-07-16 Online:2018-09-01 Published:2018-11-29
Contact: Li Xiaodong,Zhang Jinzheng
About author:
† These authors contributed equally to this paper

摘要/Abstract

摘要： 类胡萝卜素是所有光合生物及一些非光合原核生物和真菌合成的亲脂性天然色素, 对植物、动物和人都具有广泛的生物学作用。随着现代分析技术的快速发展, 越来越多的类胡萝卜素得以发现和利用。该文通过对植物类胡萝卜素的提取、分离纯化、含量测定和结构鉴定等方面的最新研究进展进行系统阐述, 以期为植物类胡萝卜素的有效开发利用提供技术支撑。

关键词: 类胡萝卜素, 提取, 分离纯化, 含量测定, 结构鉴定

Abstract: Carotenoids are lipophilic natural pigments, which synthesized by all photosynthetic organisms and some non-photosynthetic prokaryotes and fungi, and have a wide range of biological effects on plants, animals and humans. With the rapid development of modern analytical techniques, more and more carotenoids will be discovered and utilized. This article systematically elaborated the latest research progresses in the extraction, isolation and purification, content determination and structure identification of plant carotenoids in order to provide technical support for effective development and utilization of plant carotenoids.

Key words: carotenoid, extraction, isolation and purification, content determination, structure identification

何卿, 孙国峰, 林秦文, 李晓东, 张金政. 植物类胡萝卜素提取与分析技术研究进展. 植物学报, 2018, 53(5): 700-709.

He Qing, Sun Guofeng, Lin Qinwen, Li Xiaodong, Zhang Jinzheng. Advances in Extraction and Analysis Technologies of Plant Carotenoids. Chinese Bulletin of Botany, 2018, 53(5): 700-709.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB18091

https://www.chinbullbotany.com/CN/Y2018/V53/I5/700

图/表 1

参考文献 76

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提取方法	优点	缺点
溶剂提取	高提取率, 设备简单	需要大量有机溶剂且成本较高
绿色溶剂提取	环境友好, 可直接用于食品配方	成本较高且不适合分析鉴定
酶辅助提取	快速, 高效且溶剂用量少	酶的成本高
微波辅助提取	简便, 快速且经济	会导致类胡萝卜素的热降解和顺反异构化
超声波辅助提取	快速, 非热且高效	超声波探头表面老化会改变萃取效率
超临界流体萃取	溶剂非易燃、无毒且可循环利用(CO₂和乙醇); 连续萃取代替间歇处理; 可用于萃取热分子化合物; 可提供高纯度的类胡萝卜素	不适合含有大量水分的样品, 极性类胡萝卜素产率低且仪器成本高

提取方法	优点	缺点
溶剂提取	高提取率, 设备简单	需要大量有机溶剂且成本较高
绿色溶剂提取	环境友好, 可直接用于食品配方	成本较高且不适合分析鉴定
酶辅助提取	快速, 高效且溶剂用量少	酶的成本高
微波辅助提取	简便, 快速且经济	会导致类胡萝卜素的热降解和顺反异构化
超声波辅助提取	快速, 非热且高效	超声波探头表面老化会改变萃取效率
超临界流体萃取	溶剂非易燃、无毒且可循环利用(CO₂和乙醇); 连续萃取代替间歇处理; 可用于萃取热分子化合物; 可提供高纯度的类胡萝卜素	不适合含有大量水分的样品, 极性类胡萝卜素产率低且仪器成本高

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植物类胡萝卜素提取与分析技术研究进展

Advances in Extraction and Analysis Technologies of Plant Carotenoids

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