植物学报 ›› 2013, Vol. 48 ›› Issue (2): 192-198.DOI: 10.3724/SP.J.1259.2013.00192

• 技术方法 • 上一篇    下一篇

芒不同基因型愈伤组织诱导及分化的差异

刘琳1,2,3, 俞斌1,2,4, 黄鹏燕1,2,3, 贾军1,2,3, 赵华5, 彭俊华5, 陈鹏1,2,3, 彭良才1,2,3,4*   

  1. 1华中农业大学作物遗传改良国家重点实验室/武汉国家基因研究中心, 武汉 430070
    2华中农业大学生物质与生物能源研究中心, 武汉 430070;
    3华中农业大学植物科学技术学院, 武汉 430070
    4华中农业大学生命科学技术学院, 武汉 430070;
    5中国科学院武汉植物园植物种质创新与特色农业重点实验室, 武汉 430074
  • 收稿日期:2012-04-11 修回日期:2012-11-26 出版日期:2013-03-01 发布日期:2013-04-07
  • 通讯作者: 彭良才
  • 基金资助:

    973前期 (2010CB134401)

Frequency of Callus Induction and Plant Regeneration Among Eight Genotypes in Miscanthus sinensis Species

Lin Liu1,2,3, Bin Yu1,2,4, Pengyan Huang1,2,3, Jun Jia1,2,3, Hua Zhao5, Junhua Peng5, Peng Chen1,2,3, Liangcai Peng1,2,3,4*   

  1. 1National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China;

    2Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China;

    3College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;

    4College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;

    5Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China
  • Received:2012-04-11 Revised:2012-11-26 Online:2013-03-01 Published:2013-04-07
  • Contact: Liangcai Peng

摘要: 芒(Miscanthus sinensis)具较高的生物学产量, 是一种极具发展前景的纤维素类能源植物。以芒的8种不同基因型幼穗为外植体, 进行了组织培养研究。结果表明, 不同基因型芒在愈伤组织诱导率、胚性愈伤组织诱导率和胚性愈伤组织分化率等方面均存在显著差异。W89和W70均具较高的愈伤组织诱导率, 分别为91.7%和89.1%; W69的外植体几乎全部褐化, 且未能诱导出愈伤组织。W89、W70和W17的胚性愈伤组织分化率较高, 达50%以上。另外, 发现愈伤组织诱导率与细胞壁木质素含量间呈显著的负相关。该研究建立了稳定且有效的再生体系, 并初步确定W89和W70可作为芒组织培养的理想材料, 为芒的遗传转化、定向改良和良种快繁提供了技术支持。

关键词: 芒, 基因型, 组织培养, 胚性愈伤组织, 木质素

Abstract: Miscanthus sinensis has high biomass yield and is currently considered a leading energy crop candidate around the world. We performed conventional tissue culture with immature inflorescence tissue from 8 genotypes of M. sinensis and found distinct frequencies in callus induction, embryogenic-like callus induction and embryogenic-like callus differentiation. In particular, W89 and W70 showed significantly high callus induction, at 91.7% and 89.1%, respectively, whereas explants of W69 had a brownish appearance, with barely any callus. Furthermore, W89, W70 and W17 showed relatively high percentages of embryogenic-like callus differentiation (50%) with easy plantlet regeneration. Callus induction rates were affected by lignin level. The established system for efficient micropropagation could be used for genetic engineering, improvement and plant propagation of M. sinensis, and 2 genotypes, W89 and W70, may be model materials for plant tissue culture.

Key words: Miscanthus sinensis, genotype, tissue culture, embryogenic callus, lignin