柳暗花明：胞外生长素信号感受的新突破

doi:10.11983/CBB23149

摘要/Abstract

摘要： 生长素在植物生长发育过程中发挥重要作用, 其信号转导机制一直是植物学领域关注的热点。前期研究表明, ABP1-TMK分子模块参与胞外生长素信号感受, 但ABP1作为生长素受体备受争议。近期, 福建农林大学徐通达团队和杨贞标团队鉴定到ABL蛋白作为生长素结合蛋白参与胞外生长素信号感受。与传统的功能冗余不同, ABL和ABP1通过蛋白结构的相似性实现功能补偿效应, 进而与TMK在细胞膜上形成复合体, 作为胞外生长素的共受体介导生长素信号驱动的快速反应。该研究深入解析了胞外生长素信号感受的重要机制, 是生长素研究领域的重大突破。

关键词: 生长素, 生长素受体, ABL1, ABL2, ABP1, TMK

Abstract: Auxin plays an important role in plant growth and development and its signal transduction has always been the focus of attention in the field of plant biology. AUXIN BINDING PROTEIN 1 (ABP1)-TRANSMEMBRANE KINASE (TMK) molecular module is involved in the extracellular auxin perception. In recent years, ABP1 has been controversial as an auxin receptor. Recently, Tongda Xu’s team and Zhenbiao Yang’s team from Fujian Agriculture and Forestry University identified ABP1-LIKE PROTEIN (ABL) as the auxin binding proteins involved in the extracellular auxin perception. Different from traditional functional redundancy, ABL and ABP1 achieve functional compensation effect through protein structure similarity, and then form complex with TMK at the plasma membrane, acting as co-receptors of apoplastic auxin to mediate auxin driven rapid response. This study deeply dissects the mechanism of extracellular auxin sensing, which is a breakthrough in the field of auxin signaling transduction.

Key words: auxin, auxin receptor, ABL1, ABL2, ABP1, TMK

孔祥培, 张蒙悦, 丁兆军. 柳暗花明：胞外生长素信号感受的新突破. 植物学报, 2023, 58(6): 861-865.

Xiangpei Kong, Mengyue Zhang, Zhaojun Ding. There Is a Way Out-new Breakthroughs in Extracellular Auxin Sensing. Chinese Bulletin of Botany, 2023, 58(6): 861-865.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB23149

https://www.chinbullbotany.com/CN/Y2023/V58/I6/861

图/表 1

图1 ABLs/ABP1和TMKs作为胞外生长素共受体介导生长素信号驱动的快速反应模型定位于质外体的ABLs和ABP1蛋白结合生长素之后, 与TMK形成共受体复合体, 进而促进TMK磷酸化, 激活的TMK直接磷酸化一系列下游蛋白, 调控植物生长发育。ABL/ABP1-TMK介导的胞外生长素信号转导途径通过磷酸化非典型的IAA32/34与TIR1/AFBs介导的胞内生长素信号转导途径相互作用, 调控植物特定的发育过程。

Figure 1 A model of ABLs/ABP1 and TMKs acting as co-receptors of apoplastic auxin to mediate auxin driven rapid response After binding to auxin, the apoplast-localized ABLs/ABP1 form a co-receptor complex with TMK, which further promotes the phosphorylation of TMK, and the activated TMK kinase domain directly phosphorylates a series of effectors that regulate many developmental processes. The ABL/ABP1-TMK-mediated extracellular auxin signal interacts with TIR1/AFBs mediated intracellular auxin signal transduction pathway to regulate specific developmental processes by phosphorylating non-canonical IAA32/34.

参考文献 23

[1]	Braun N, Wyrzykowska J, Muller P, David K, Couch D, Perrot-Rechenmann C, Fleming AJ (2008). Conditional repression of AUXIN BINDING PROTEIN 1 reveals that it coordinates cell division and cell expansion during postembryonic shoot development in Arabidopsis and tobacco. Plant Cell 20, 2746-2762. DOI PMID
[2]	Cao M, Chen R, Li P, Yu YQ, Zheng R, Ge DF, Zheng W, Wang XH, Gu YT, Gelová Z, Friml J, Zhang H, Liu RY, He J, Xu TD (2019). TMK1-mediated auxin signaling regulates differential growth of the apical hook. Nature 568, 240-243. DOI
[3]	Chen HH, Li LX, Zou MX, Qi LL, Friml J (2023). Distinct functions of TIR1 and AFB1 receptors in auxin signaling. Mol Plant 16, 1117-1119. DOI PMID
[4]	Chen JG, Ullah H, Young JC, Sussman MR, Jones AM (2001). ABP1 is required for organized cell elongation and division in Arabidopsis embryogenesis. Genes Dev 15, 902-911. DOI URL
[5]	Cui XK, Wang JX, Li K, Lv BS, Hou BK, Ding ZJ (2023). Protein post-translational modifications in auxin signaling. J Genet Genomics doi: 10.1016/j.jgg.2023.07.002.
[6]	Dai XH, Zhang Y, Zhang D, Chen JL, Gao XH, Estelle M, Zhao YD (2015). Embryonic lethality of Arabidopsis abp1-1 is caused by deletion of the adjacent BSM gene. Nat Plants 1, 15183. DOI
[7]	Dharmasiri N, Dharmasiri S, Estelle M (2005). The F-box protein TIR1 is an auxin receptor. Nature 435, 441-445. DOI
[8]	Dubey SM, Han S, Stutzman N, Prigge MJ, Medvecká E, Platre MP, Busch W, Fendrych M, Estelle M (2023). The AFB1 auxin receptor controls the cytoplasmic auxin response pathway in Arabidopsis thaliana. Mol Plant 16, 1120-1130. DOI PMID
[9]	Fendrych M, Leung J, Friml J (2016). TIR1/AFB-Aux/IAA auxin perception mediates rapid cell wall acidification and growth of Arabidopsis hypocotyls. eLife 5, e19048. DOI URL
[10]	Friml J, Gallei M, Gelová Z, Johnson A, Mazur E, Monzer A, Rodriguez L, Roosjen M, Verstraeten I, Živanović BD, Zou MX, Fiedler L, Giannini C, Grones P, Hrtyan M, Kaufmann WA, Kuhn A, Narasimhan M, Randuch M, Rýdza N, Takahashi K, Tan ST, Teplova A, Kinoshita T, Weijers D, Rakusová H (2022). ABP1-TMK auxin perception for global phosphorylation and auxin canalization. Nature 609, 575-581. DOI
[11]	Gao YB, Zhang Y, Zhang D, Dai XH, Estelle M, Zhao YD (2015). Auxin binding protein 1 (ABP1) is not required for either auxin signaling or Arabidopsis development. Proc Natl Acad Sci USA 112, 2275-2280. DOI URL
[12]	Grones P, Chen X, Simon S, Kaufmann WA, De Rycke R, Nodzyński T, Zažímalová E, Friml J (2015). Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles. J Exp Bot 66, 5055-5065. DOI PMID
[13]	Kepinski S, Leyser O (2005). The Arabidopsis F-box protein TIR1 is an auxin receptor. Nature 435, 446-451. DOI
[14]	Li LX, Verstraeten I, Roosjen M, Takahashi K, Rodriguez L, Merrin J, Chen J, Shabala L, Smet W, Ren H, Vanneste S, Shabala S, De Rybel B, Weijers D, Kinoshita T, Gray WM, Friml J (2021). Cell surface and intracellular auxin signaling for H⁺ fluxes in root growth. Nature 599, 273-277. DOI
[15]	Qi LL, Kwiatkowski M, Chen HH, Hoermayer L, Sinclair S, Zou MX, del Genio CI, Kubeš MF, Napier R, Jaworski K, Friml J (2022). Adenylate cyclase activity of TIR1/AFB auxin receptors in plants. Nature 611, 133-138. DOI
[16]	Robert S, Kleine-Vehn J, Barbez E, Sauer M, Paciorek T, Baster P, Vanneste S, Zhang J, Simon S, Čovanová M, Hayashi K, Dhonukshe P, Yang ZB, Bednarek SY, Jones AM, Luschnig C, Aniento F, Zažímalová E, Friml J (2010). ABP1 mediates auxin inhibition of clathrin-dependent endocytosis in Arabidopsis. Cell 143, 111-121. DOI URL
[17]	Serre NBC, Kralík D, Yun P, Slouka Z, Shabala S, Fendrych M (2022). AFB1 controls rapid auxin signaling through membrane depolarization in Arabidopsis thaliana root. Nat Plants 8, 1317. DOI
[18]	Tromas A, Braun N, Muller P, Khodus T, Paponov IA, Palme K, Ljung K, Lee JY, Benfey P, Murray JAH, Scheres B, Perrot-Rechenmann C (2009). The AUXIN BINDING PROTEIN 1 is required for differential auxin responses mediating root growth. PLoS One 4, e6648. DOI URL
[19]	Woo EJ, Marshall J, Bauly J, Chen JG, Venis M, Napier RM, Pickersgill RW (2002). Crystal structure of auxin- binding protein 1 in complex with auxin. EMBO J 21, 2877-2885. DOI URL
[20]	Xu TD, Dai N, Chen JS, Nagawa S, Cao M, Li HJ, Zhou ZM, Chen X, De Rycke R, Rakusová H, Wang WY, Jones AM, Friml J, Patterson SE, Bleecker AB, Yang ZB (2014). Cell surface ABP1-TMK auxin-sensing complex activates ROP GTPase signaling. Science 343, 1025-1028. DOI PMID
[21]	Xu TD, Wen MZ, Nagawa S, Fu Y, Chen JG, Wu MJ, Perrot-Rechenmann C, Friml J, Jones AM, Yang ZB (2010). Cell surface- and Rho GTPase-based auxin signaling controls cellular interdigitation in Arabidopsis. Cell 143, 99-110. DOI URL
[22]	Yu YQ, Tang WX, Lin WW, Zhou X, Li Y, Chen R, Zheng R, Qin GC, Cao WH, Pérez-Henríquez P, Huang RF, Ma J, Qiu QQ, Xu ZW, Zou AL, Lin JC, Jiang LW, Xu TD, Yang ZB (2023). ABLs and TMKs are co-receptors for extracellular auxin. Cell doi: 10.1016/j.cell.2023.10.017.
[23]	Yu ZP, Zhang F, Friml J, Ding ZJ (2022). Auxin signaling: research advances over the past 30 years. J Integr Plant Biol 64, 371-392. DOI