Chin Bull Bot ›› 2019, Vol. 54 ›› Issue (5): 547-549.doi: 10.11983/CBB19166

• COMMENTARIES •     Next Articles

Discovery of ZmFBL41 Chang7-2 as A Key Weapon against Banded Leaf and Sheath Blight Resistance in Maize

Li Weitao,He Min,Chen Xuewei()   

  1. Rice Research Institute, State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China
  • Received:2019-08-27 Accepted:2019-09-17 Online:2019-01-01 Published:2019-09-01
  • Contact: Chen Xuewei E-mail:xwchen88@163.com

Abstract:

The fungal pathogen Rhizoctonia solani causes banded leaf and sheath blight (BLSB) in maize (Zea mays) and sheath blight (ShB) in rice (Oryza sativa). R. solani has a wide range of host and severely threatens crop production. The lack of resistant resources against BLSB and the poor understanding of disease resistance mechanism hamper the development of effective approaches to control this fungal disease. Recently, Chinese scientists have made a breakthrough discovery that an F-box protein ZmFBL41 mediates the proteasomal degradation of cinnamyl-alcohol dehydrogenase ZmCAD to regulate BLSB and ShB disease resistance. By genome-wide association analysis, GRMZM2G 109140 (ZmFBL41) was identified as a major QTL candidate gene associated with BLSB disease resistance. ZmFBL41 protein is a member of SKP1-Cullin-F-box (SCF) E3 ubiquitin ligase complex which mediates the degradation of ZmCAD, thus reducing the accumulation of lignin and rendering maize more susceptible to R. solani. Interestingly, in the maize inbred line Chang7-2, the natural variation on two amino acids in ZmFBL41 Chang7-2 results in resistance against BLSB. Mechanistically, ZmFBL41 Chang7-2 fails to interact with and degrade its substrate ZmCAD, leading to the accumulation of lignin, which consequently enhances maize resistance. This study not only discovers a novel molecular mechanism underlying disease resistance of maize against R. solani, but also provides important theoretical basis and genetic resources for breeding maize and other crops with improved disease resistance.

Key words: plant immunity, SKP1-Cullin-F-box, lignin, banded leaf and sheath blight, maize, rice

Figure 1

A model for ZmFBL41-mediated banded leaf and sheath blight (BLSB) resistance ZmFBL41 interacts with ZmSKP1-1 to form the SCF complex, and recruits ZmCAD for 26S proteasome-mediated degradation, resulting in reduced lignin synthesis and increased susceptibility of maize to R. solani. However, in the natural maize resource Chang7-2, the protein ZmFBL41Chang7-2 with two amino acid variations (E214G and S217R) is not able to interact with ZmCAD, leading to failure in degradation of ZmCAD and resulting in accumulation of lignin, which consequently enhances resistance to R. solani."

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