Chin Bull Bot ›› 2019, Vol. 54 ›› Issue (3): 385-395.doi: 10.11983/CBB18151

• SPECIAL TOPICS • Previous Articles     Next Articles

Recent Progress in Evolutionary Technology of CRISPR/Cas9 System for Plant Genome Editing

Su Yuekai,Qiu Jingren,Zhang Han,Song Zhenqiao,Wang Jianhua()   

  1. College of Agronomy, Shandong Agricultural University, Tai’an 271018, China
  • Received:2018-07-02 Accepted:2018-08-09 Online:2019-11-24 Published:2019-05-01
  • Contact: Wang Jianhua E-mail:jhwangjh@163.com

Abstract:

CRISPR/Cas9 genome editing is used for precisely modifying the genome, enabling nucleotide insertion, deletion, or DNA fragment replacement in targeted gene(s). With more understanding of the CRISPR/Cas9 system, this technology has been widely applied in research, agriculture, medical treatment and other fields. This article briefly introduces the discovery and working principle of the CRISPR/Cas9 genome-editing technology, and summarizes the research progress in optimizing and improving the technology in recent years, including improving the gene editing efficiency, the range expansion of gene editing, base editing and multigene editing, the safety enhancement of genome editing, replacing gene fragments and transcriptional regulation of targeted genes, to provide references for thorough research work in this area.

Key words: CRISPR/Cas9, genome editing, genetic improvement, plant genomes

Figure 1

The schematic diagram of CRISPR/Cas9 genome editing (Jiang and Doudna, 2017)The Cas9 protein from Streptococcus pyogenes (SpCas9) and associated guide RNA (sgRNA), containing a 20 nt recognition sequence, will cleave a target sequence located upstream of the protospacer adjacent motif (PAM) region, resulting in double-strand break (DSB). Ultimately use the repair in the organism to achieve genome editing."

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