Advances in Haploid Breeding Technology and Its Application in Alfalfa and Other Legume Forages

doi:10.11983/CBB22190

Abstract

Abstract: Doubled haploid breeding can achieve rapid homogenization of genetic material through the combination of haploid induction and doubling technology, which significantly accelerates the speed of pure line production and the selection process in hybrid breeding, is a common key technology in agricultural breeding. In this paper, we briefly review the methods and progress of plant haploid induction and chromosome doubling, introduce the latest gene editing-mediated in vivo haploid induction technology, and discuss and prospect the application of haploid breeding technology in Medicago sativa and other legumes forage.

Key words: haploid breeding technology, genome editing technology, legume forage, alfalfa

Na Wang, Teng Jiang, Binxi Wang, Lifang Niu, Hao Lin. Advances in Haploid Breeding Technology and Its Application in Alfalfa and Other Legume Forages[J]. Chinese Bulletin of Botany, 2022, 57(6): 756-763.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB22190

https://www.chinbullbotany.com/EN/Y2022/V57/I6/756

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References 55

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目的基因	植物物种	基因编辑类型	单倍体比例(%)	参考文献
AtCENH3	Arabidopsis thaliana	Gene replacement and insertion	25-45	2010
ZmCENH3	Zea mays	Gene replacement and insertion/RNAi	0.16-3.6	2016
TaCENH3	Triticum aestivum	Gene replacement and insertion	7	2020
ZmCENH3	Z. mays	Site-specified knockout	0.5-5	2021
ZmMTL	Z. mays	TALENs	6.7	2017
ZmPLA1	Z. mays	Site-specified knockout	1.55-6.67	2017
ZmNLD	Z. mays	Gene replacement and insertion	0.5-3.59	2017a
OsMATL	Oryza sativa	Site-specified knockout	2-6	2018
TaPLA	T. aestivum	Site-specified knockout	5.88-15.66	2020a
TaMTL	T. aestivum	Site-specified knockout	11.8-31.6	2020b
SiMTL	Setaria italica	Site-specified knockout	1.75-3.49	2021
ZmDMP	Z. mays	Site-specified knockout	0.1-0.3	2019
AtDMP8/9	A. thaliana	Site-specified knockout	0.92-3.23	2020
MtDMP8/9	Medicago truncatula	Site-specified knockout	0.29-0.82	2022
SlDMP	Solanum lycopersicum	Site-specified knockout	0.49-3.68	2022a
BnaDMP	Brassica napus	Site-specified knockout	2.4	2022b
ZmPLD3	Z. mays	Site-specified knockout	0.85-0.96	2021
ZmPOD65	Z. mays	Site-specified knockout	0.9-7.7	2022

目的基因	植物物种	基因编辑类型	单倍体比例(%)	参考文献
AtCENH3	Arabidopsis thaliana	Gene replacement and insertion	25-45	2010
ZmCENH3	Zea mays	Gene replacement and insertion/RNAi	0.16-3.6	2016
TaCENH3	Triticum aestivum	Gene replacement and insertion	7	2020
ZmCENH3	Z. mays	Site-specified knockout	0.5-5	2021
ZmMTL	Z. mays	TALENs	6.7	2017
ZmPLA1	Z. mays	Site-specified knockout	1.55-6.67	2017
ZmNLD	Z. mays	Gene replacement and insertion	0.5-3.59	2017a
OsMATL	Oryza sativa	Site-specified knockout	2-6	2018
TaPLA	T. aestivum	Site-specified knockout	5.88-15.66	2020a
TaMTL	T. aestivum	Site-specified knockout	11.8-31.6	2020b
SiMTL	Setaria italica	Site-specified knockout	1.75-3.49	2021
ZmDMP	Z. mays	Site-specified knockout	0.1-0.3	2019
AtDMP8/9	A. thaliana	Site-specified knockout	0.92-3.23	2020
MtDMP8/9	Medicago truncatula	Site-specified knockout	0.29-0.82	2022
SlDMP	Solanum lycopersicum	Site-specified knockout	0.49-3.68	2022a
BnaDMP	Brassica napus	Site-specified knockout	2.4	2022b
ZmPLD3	Z. mays	Site-specified knockout	0.85-0.96	2021
ZmPOD65	Z. mays	Site-specified knockout	0.9-7.7	2022