Chinese Bulletin of Botany ›› 2018, Vol. 53 ›› Issue (4): 528-541.DOI: 10.11983/CBB18004

• SPECIAL TOPICS • Previous Articles     Next Articles

Research Progress in Off-target in CRISPR/Cas9 Genome Editing

Wang Ying1,2,*(), Li Xianggan2, Qiu Lijuan1,*()   

  1. 1Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    2Syngenta Biotechnology China Co, LTD, Beijing 102206, China
  • Received:2018-01-03 Accepted:2018-03-29 Online:2018-07-01 Published:2018-09-11
  • Contact: Wang Ying,Qiu Lijuan
  • About author:† These authors contributed equally to this paper

Abstract: CRISPR/Cas9 technology has rapidly developed in recent years. It is widely used for study of animals, plants and microorganisms. Among all developments, the use of the technology for the off-target issue has progressed, with important breakthroughs for this hot topic. This paper elucidates the principle of off-target generation and the method for detecting off target both in vitro and in vivo. Also, how to reduce off-targets by improving single-guide RNA design and optimizing the CRISPR system is evaluated. Further more, detecting off-targets at the right time and improving the precision and accuracy of off-target detection are proposed for plant site-specific genome editing.

Key words: CRISPR/Cas9, site specific editing, off-target