Advances in Virus-mediated Genome Editing Technology in Plants

doi:10.11983/CBB23046

Abstract

Abstract: As a new technology for targeted genome editing, clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated protein (Cas) have the advantages of easy operation, high editing efficiency, and support for multi-target editing, thus showing wide application prospects in plant genetic breeding. However, the process in plants relies mainly on Agrobacterium- or particle bombardment-mediated genetic transformation, which is time-consuming as well as species- and varieties-dependent. Virus-mediated plant genome editing has attracted extensive atten- tion because of its no requirement of genetic transformation and plant regeneration. In this review, we introduce the working principle and advantages of virus-mediated CRISPR/Cas plant genome editing technology, systematically sum- marize the current application status of this technology in the field of plant genome editing, and focus on discussing the problems and challenges of this technology system, aiming to provide reference for further research and development in this field.

Key words: virus, plant, genome editing, CRISPR/Cas

Danling Hu, Yongwei Sun. Advances in Virus-mediated Genome Editing Technology in Plants[J]. Chinese Bulletin of Botany, 2024, 59(3): 452-462.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB23046

https://www.chinbullbotany.com/EN/Y2024/V59/I3/452

Figures/Tables 2

References 65

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病毒类型	病毒名称	受体植物	递送成分	非组织培养稳定遗传效率	参考文献
正链RNA病毒	烟草脆裂病毒	本氏烟草(Nicotiana benthamiana) (Cas9过表达)	sgRNAs	0.2%	Ali et al., 2015a
	烟草脆裂病毒和豌豆早枯病毒	本氏烟草和拟南芥(Arabidopsis thaliana) (Cas9过表达)	sgRNAs	N.A	Ali et al., 2015b, 2018
	烟草脆裂病毒	本氏烟草(Cas9过表达)	sgRNAs和可移动sgRNAs	65%-100%	Ellison et al., 2020
	烟草脆裂病毒	拟南芥(过表达SunTag系统)	sgRNAs和可移动 sgRNAs	5%-8%	Ghoshal et al., 2020
	烟草脆裂病毒	拟南芥(Cas9过表达)	sgRNAs和可移动sgRNAs	39%-60%	Nagalakshmi et al., 2022
	烟草脆裂病毒	烟草(N. tabacum) (Cas9过表达)	sgRNAs	N.A	Oh et al., 2022
	烟草脆裂病毒和豌豆早枯病毒	烟草(Cas9过表达)	sgRNAs	N.A	Kim et al., 2023
	烟草花叶病毒	本氏烟草(Cas9过表达)	Cas9和sgRNAs	N.A	Cody et al., 2017
	烟草花叶病毒	本氏烟草	Cas9和sgRNAs	N.A	Chiong et al., 2021
	番茄花叶病毒	本氏烟草(分开的SaCas9s部分过表达)	部分Split-SaCas9s和sgRNAs	N.A	Kaya et al., 2017
	苹果潜隐球形病毒	本氏烟草和大豆(Glycine max) (Cas9, Csy4过表达)	sgRNAs	N.A	Luo et al., 2021
	甜菜坏死黄脉病毒	本氏烟草(Cas9过表达)	sgRNAs	N.A	Jiang et al., 2019
	大麦条纹花叶病毒	本氏烟草、小麦(Triticum aestivum)和玉米(Zea mays) (Cas9过表达)	sgRNAs	N.A	Hu et al., 2019
	大麦条纹花叶病毒	小麦(Cas9过表达)	sgRNAs和可移动sgRNAs	0-17%	Wang et al., 2022
	大麦条纹花叶病毒	本氏烟草和小麦(Cas9过表达)	sgRNAs和可移动sgRNAs	12.9%-100%	Li et al., 2021
	大麦条纹花叶病毒	本氏烟草和小麦(Cas9过表达)	sgRNAs和可移动sgRNAs	0.5%-0.8%	Chen et al., 2022
	大麦条纹花叶病毒	本氏烟草和大麦(Hordeum vulgare) (Cas9过表达)	sgRNAs	100%	Tamilselvan-Nattar- Amutha et al., 2023
	马铃薯X病毒	本氏烟草	Cas9和sgRNAs	N.A	Ariga et al., 2020
	马铃薯X病毒	本氏烟草(Cas9过表达)	sgRNAs和可移动sgRNAs	22%	Uranga et al., 2021
	狗尾草花叶病毒	本氏烟草	Cas9和sgRNAs	N.A	Zhang et al., 2020
	狗尾草花叶病毒	本氏烟草、玉米和狗尾草(Setaria viridis) (Cas9过表达)	sgRNAs	N.A	Mei et al., 2019
负链RNA 病毒	苦苣菜黄网弹状病毒	本氏烟草	Cas9和sgRNAs	N.A	Ma et al., 2020
	大麦黄条纹花叶病毒	本氏烟草	Cas9和sgRNAs	N.A	Gao et al., 2019
	番茄斑萎病毒	本氏烟草、番茄(Solanum lycopersicum)、辣椒(Capsicum annuum)、灯笼果(Physalis peruviana)和花生(Arachis hypogaea)	Cas9和sgRNAs, Cas12a和crRNA	N.A	Liu et al., 2023
DNA 病毒	甘蓝曲叶病毒	本氏烟草(Cas9过表达)	sgRNAs	N.A	Yin et al., 2015
	棉皱叶病毒	拟南芥(Cas9过表达)	sgRNAs和可移动sgRNAs	4.35%-8.79%	Lei et al., 2021
	棉皱叶病毒	棉花(Gossypium spp.) (Cas9过表达)	sgRNAs和可移动sgRNAs	N.A	Lei et al., 2022

病毒类型	病毒名称	受体植物	递送成分	非组织培养稳定遗传效率	参考文献
正链RNA病毒	烟草脆裂病毒	本氏烟草(Nicotiana benthamiana) (Cas9过表达)	sgRNAs	0.2%	Ali et al., 2015a
	烟草脆裂病毒和豌豆早枯病毒	本氏烟草和拟南芥(Arabidopsis thaliana) (Cas9过表达)	sgRNAs	N.A	Ali et al., 2015b, 2018
	烟草脆裂病毒	本氏烟草(Cas9过表达)	sgRNAs和可移动sgRNAs	65%-100%	Ellison et al., 2020
	烟草脆裂病毒	拟南芥(过表达SunTag系统)	sgRNAs和可移动 sgRNAs	5%-8%	Ghoshal et al., 2020
	烟草脆裂病毒	拟南芥(Cas9过表达)	sgRNAs和可移动sgRNAs	39%-60%	Nagalakshmi et al., 2022
	烟草脆裂病毒	烟草(N. tabacum) (Cas9过表达)	sgRNAs	N.A	Oh et al., 2022
	烟草脆裂病毒和豌豆早枯病毒	烟草(Cas9过表达)	sgRNAs	N.A	Kim et al., 2023
	烟草花叶病毒	本氏烟草(Cas9过表达)	Cas9和sgRNAs	N.A	Cody et al., 2017
	烟草花叶病毒	本氏烟草	Cas9和sgRNAs	N.A	Chiong et al., 2021
	番茄花叶病毒	本氏烟草(分开的SaCas9s部分过表达)	部分Split-SaCas9s和sgRNAs	N.A	Kaya et al., 2017
	苹果潜隐球形病毒	本氏烟草和大豆(Glycine max) (Cas9, Csy4过表达)	sgRNAs	N.A	Luo et al., 2021
	甜菜坏死黄脉病毒	本氏烟草(Cas9过表达)	sgRNAs	N.A	Jiang et al., 2019
	大麦条纹花叶病毒	本氏烟草、小麦(Triticum aestivum)和玉米(Zea mays) (Cas9过表达)	sgRNAs	N.A	Hu et al., 2019
	大麦条纹花叶病毒	小麦(Cas9过表达)	sgRNAs和可移动sgRNAs	0-17%	Wang et al., 2022
	大麦条纹花叶病毒	本氏烟草和小麦(Cas9过表达)	sgRNAs和可移动sgRNAs	12.9%-100%	Li et al., 2021
	大麦条纹花叶病毒	本氏烟草和小麦(Cas9过表达)	sgRNAs和可移动sgRNAs	0.5%-0.8%	Chen et al., 2022
	大麦条纹花叶病毒	本氏烟草和大麦(Hordeum vulgare) (Cas9过表达)	sgRNAs	100%	Tamilselvan-Nattar- Amutha et al., 2023
	马铃薯X病毒	本氏烟草	Cas9和sgRNAs	N.A	Ariga et al., 2020
	马铃薯X病毒	本氏烟草(Cas9过表达)	sgRNAs和可移动sgRNAs	22%	Uranga et al., 2021
	狗尾草花叶病毒	本氏烟草	Cas9和sgRNAs	N.A	Zhang et al., 2020
	狗尾草花叶病毒	本氏烟草、玉米和狗尾草(Setaria viridis) (Cas9过表达)	sgRNAs	N.A	Mei et al., 2019
负链RNA 病毒	苦苣菜黄网弹状病毒	本氏烟草	Cas9和sgRNAs	N.A	Ma et al., 2020
	大麦黄条纹花叶病毒	本氏烟草	Cas9和sgRNAs	N.A	Gao et al., 2019
	番茄斑萎病毒	本氏烟草、番茄(Solanum lycopersicum)、辣椒(Capsicum annuum)、灯笼果(Physalis peruviana)和花生(Arachis hypogaea)	Cas9和sgRNAs, Cas12a和crRNA	N.A	Liu et al., 2023
DNA 病毒	甘蓝曲叶病毒	本氏烟草(Cas9过表达)	sgRNAs	N.A	Yin et al., 2015
	棉皱叶病毒	拟南芥(Cas9过表达)	sgRNAs和可移动sgRNAs	4.35%-8.79%	Lei et al., 2021
	棉皱叶病毒	棉花(Gossypium spp.) (Cas9过表达)	sgRNAs和可移动sgRNAs	N.A	Lei et al., 2022