植物学报 ›› 2014, Vol. 49 ›› Issue (3): 246-253.doi: 10.3724/SP.J.1259.2014.00246

• 研究报告 • 上一篇    下一篇

应用基因芯片分析甘蓝型油菜柱头特异表达基因

李春宏, 付三雄, 戚存扣*   

  1. 江苏省农业科学院经济作物研究所, 国家油菜改良中心南京分中心, 南京 210014
  • 收稿日期:2013-08-05 修回日期:2013-10-20 出版日期:2014-05-01 发布日期:2014-06-03
  • 通讯作者: 戚存扣 E-mail:qckjaas@gmail.com
  • 基金资助:

    CRABS CLAW(CRC)在油菜花发育过程中的调控机理研究;甘蓝型油菜突变体FS-M1雌性不育机理及其相关基因克隆

Microarray Analysis of Genes Expressed in Brassica napus Stigma

Chunhong Li, Sanxiong Fu, Cunkou Qi*   

  1. Nanjing Sub-center of National Rapeseed Development Center, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
  • Received:2013-08-05 Revised:2013-10-20 Online:2014-05-01 Published:2014-06-03
  • Contact: Cunkou Qi E-mail:qckjaas@gmail.com

摘要: 以甘蓝型油菜(Brassica napus)野生型(宁油10号)及其柱头授粉功能缺失突变体FS-M1为材料, 使用油菜基因表达谱芯片筛选甘蓝型油菜柱头特异表达基因。在含有16 540个基因的油菜基因表达谱芯片中(43 803探针), 获得了4 410条差异表达探针, 选择部分差异表达基因进行实时定量PCR, 所得结果与芯片检测结果相吻合。其中, 野生型较FS-M1显著上调且获得209个功能注释的探针, 对应198个基因, 这些特异表达的基因主要富集在水解酶、转移酶、氧化还原酶和转录因子中; 涉及较大的基因家族包括: 细胞色素P450基因、GDSL脂肪酶/水解酶基因、ABC转运蛋白基因、myb转录因子基因、bHLH转录因子基因、过氧化物酶家族和受体激酶基因等。推测这些基因与甘蓝型油菜柱头发育及授粉功能有关。

Abstract: Genes expressed in stigma were screened for a pollination-deficient mutant FS-M1 in Brassica napus and its wild type (NY10) by using B. napus gene microarray. Among the 16 540 genes (43 803 probes) represented on the array, 4 410 showed significant differential expression; the differential expression of selected genes was validated by qRT-PCR. We identified 198 genes (209 probes) that were significantly upregulated in the wild-type stigma and were markedly enriched in function of hydrolase activity, transferase activity, oxidoreductase activity and transcription factors, and some considerable gene families were involved, including cytochrome P450, GDSL-motif lipase, ABC transporter, myb transcription factor, bHLH transcription factor, peroxidase and receptor kinase, which may be related to development and pollination of B. napus stigma.

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