Chinese Bulletin of Botany ›› 2025, Vol. 60 ›› Issue (3): 435-448.DOI: 10.11983/CBB24189 cstr: 32102.14.CBB24189
• SPECIAL TOPICS • Previous Articles Next Articles
Xiong Lianglin1,2, Liang Guolu1,2, Guo Qigao1,2, Jing Danlong1,2,*(
)
HomeReceived:2024-12-05
Accepted:2025-02-22
Online:2025-05-10
Published:2025-02-26
Contact:
*E-mail: jingdanlong@swu.edu.cn
Xiong Lianglin, Liang Guolu, Guo Qigao, Jing Danlong. Advances in the Regulation of Alternative Splicing of Genes in Plants in Response to Abiotic Stress[J]. Chinese Bulletin of Botany, 2025, 60(3): 435-448.
Figure 1 Main types of alternative splicing under abiotic stress (refer to Laloum et al., 2018)
Figure 2 The mechanism of precursor mRNA regulation mediated by serine/arginine-rich (SR) proteins under normal conditions and different degrees of heat stress (refer to Ling et al., 2021) (A) Schematic diagram of constitutive splicing in plants under normal development (under normal conditions, heat-responsive genes (HRG) are not activated or are barely expressed, while growth-related genes (GRGs) are actively transcribed. SR proteins are efficiently phosphorylated (e.g., phosphorylated by SR protein kinase (SRPK) in the splicing factor complex (SFC)), thus participating in constitutive splicing and generating many development-related proteins); (B) Schematic diagram of alternative splicing in plants under mild heat stress (under mild heat stress (e.g., 32°C for 6 hours), HRGs including heat-inducible SR genes are transcriptionally activated. Phosphorylated SR proteins participate in constitutive or alternative splicing of HRGs. GRGs are suppressed at the transcriptional level, but their pre-mRNAs may still be alternative spliced by phosphorylated SR proteins to produce functionally distinct isoforms. Through this mechanism, plant cells produce a large number of heat-tolerant proteins to cope with heat stress); (C) Schematic diagram of alternative splicing in plants under severe heat stress (under severe heat stress (e.g., 45°C for 90 minutes), HRGs including heat-inducible SR genes are activated at the transcriptional level. However, under severe heat stress, the dephosphorylation of SR proteins (e.g., caused by the deficiency of SRPK) leads to a deficiency of active splicing factors, resulting in the accumulation of intron-retained transcripts of HRGs and GRGs in the nucleus, and leads to an extremely low number of functional transcripts. Therefore, there is a shortage of heat-tolerant proteins and development-related proteins in plant cells). ASF: Alternative spliced fragment; ×: The process is inhibited.
| 物种 | 基因 | 功能 | 胁迫类型 | 参考文献 |
|---|---|---|---|---|
| 拟南芥(Arabidopsis thaliana) | AtHsfA2 | 维持热休克蛋白相关基因的表达水平 | 热胁迫 | Charng et al., |
| AtSIZ1 | 促进热休克蛋白相关基因表达 | 热胁迫 | Ishida et al., | |
| AtIRE1 | 通过调节转录因子bZIP60前体mRNA剪接上调BIP3蛋白的表达 | 热胁迫 | Ling et al., | |
| AtZIFL1 | 产生剪接变体, 靶向叶片气孔保卫细胞的质膜并介导耐旱性 | 干旱胁迫 | Remy et al., | |
| AtSPCH | 降低气孔密度, 减少水分蒸发 | 干旱胁迫 | Wang et al., | |
| AtSR | 使剪接因子丰度和活性发生动态变化, 影响靶基因表达 | 冷胁迫 | Syed et al., | |
| AtLUC7 | 参与冷胁迫条件下内含子的剪接调节 | 冷胁迫 | de Francisco Amorim et al., | |
| AtSR45 | SR45.1亚型通过调节SOS基因表达以控制离子稳态和赋予耐盐性 | 盐胁迫 | Albaqami et al., | |
| AtSR34b | 通过IRT1的剪接和稳定性促进IRT1蛋白积累 | 盐胁迫 | Zhang et al., | |
| AtSAD1 | 提高盐胁迫响应基因的剪接效率 | 盐胁迫 | Cui et al., | |
| AtSKIP | 参与剪接位点的识别或切割 | 盐胁迫 | Feng et al., | |
| AtRCD1 | RCD1的剪接变体可减少盐诱导的细胞死亡 | 盐胁迫 | Hong et al., | |
| 水稻(Oryza sativa) | OsIRE1 | 调节热胁迫响应基因OsbZIP74的前体mRNA剪接 | 热胁迫 | Ling et al., |
| 小麦(Triticum aestivum) | TaHSFA6e | TaHSFA6e的剪接变体可增强下游热休克蛋白基因的转录活性 | 热胁迫 | Wen et al., |
| 毛白杨(Populus tomentosa) | PtoRSZ21 | 通过调节PtoATG2b的剪接影响植物的水分利用效率和抗逆能力 | 干旱胁迫 | Huang et al., |
| 玉米(Zea mays) | ZmPP2C26 | ZmPP2C26的2个剪接变体负向调节干旱耐受性 | 干旱胁迫 | Lu et al., |
| 茶树(Camellia sinensis) | CsWRKY21 | CsWRKY21的剪接变体抑制ABA分解相关基因的表达 | 冷胁迫 | Mi et al., |
| 谷子(Setaria italica) | SiCYP19 | 剪接变体可以提高脯氨酸含量和促进活性氧清除 | 盐胁迫 | Zhang et al., |
| 胡杨(Populus euphratica) | PeuHKT1;3 | 产生PeuHKT1;3a变体, 改变离子选择性 | 盐胁迫 | Lv et al., |
| 大豆(Glycine max) | GmPeNTL9 | 剪接变体激活抗氧化清除系统 | 盐胁迫 | Liu et al., |
| GmSnRK1.1 | 调节转录因子的活性和稳定性 | 盐胁迫 | Liu et al., |
Table 1 Alternative splicing-related genes involved in regulating plant responses to abiotic stress in different species
| 物种 | 基因 | 功能 | 胁迫类型 | 参考文献 |
|---|---|---|---|---|
| 拟南芥(Arabidopsis thaliana) | AtHsfA2 | 维持热休克蛋白相关基因的表达水平 | 热胁迫 | Charng et al., |
| AtSIZ1 | 促进热休克蛋白相关基因表达 | 热胁迫 | Ishida et al., | |
| AtIRE1 | 通过调节转录因子bZIP60前体mRNA剪接上调BIP3蛋白的表达 | 热胁迫 | Ling et al., | |
| AtZIFL1 | 产生剪接变体, 靶向叶片气孔保卫细胞的质膜并介导耐旱性 | 干旱胁迫 | Remy et al., | |
| AtSPCH | 降低气孔密度, 减少水分蒸发 | 干旱胁迫 | Wang et al., | |
| AtSR | 使剪接因子丰度和活性发生动态变化, 影响靶基因表达 | 冷胁迫 | Syed et al., | |
| AtLUC7 | 参与冷胁迫条件下内含子的剪接调节 | 冷胁迫 | de Francisco Amorim et al., | |
| AtSR45 | SR45.1亚型通过调节SOS基因表达以控制离子稳态和赋予耐盐性 | 盐胁迫 | Albaqami et al., | |
| AtSR34b | 通过IRT1的剪接和稳定性促进IRT1蛋白积累 | 盐胁迫 | Zhang et al., | |
| AtSAD1 | 提高盐胁迫响应基因的剪接效率 | 盐胁迫 | Cui et al., | |
| AtSKIP | 参与剪接位点的识别或切割 | 盐胁迫 | Feng et al., | |
| AtRCD1 | RCD1的剪接变体可减少盐诱导的细胞死亡 | 盐胁迫 | Hong et al., | |
| 水稻(Oryza sativa) | OsIRE1 | 调节热胁迫响应基因OsbZIP74的前体mRNA剪接 | 热胁迫 | Ling et al., |
| 小麦(Triticum aestivum) | TaHSFA6e | TaHSFA6e的剪接变体可增强下游热休克蛋白基因的转录活性 | 热胁迫 | Wen et al., |
| 毛白杨(Populus tomentosa) | PtoRSZ21 | 通过调节PtoATG2b的剪接影响植物的水分利用效率和抗逆能力 | 干旱胁迫 | Huang et al., |
| 玉米(Zea mays) | ZmPP2C26 | ZmPP2C26的2个剪接变体负向调节干旱耐受性 | 干旱胁迫 | Lu et al., |
| 茶树(Camellia sinensis) | CsWRKY21 | CsWRKY21的剪接变体抑制ABA分解相关基因的表达 | 冷胁迫 | Mi et al., |
| 谷子(Setaria italica) | SiCYP19 | 剪接变体可以提高脯氨酸含量和促进活性氧清除 | 盐胁迫 | Zhang et al., |
| 胡杨(Populus euphratica) | PeuHKT1;3 | 产生PeuHKT1;3a变体, 改变离子选择性 | 盐胁迫 | Lv et al., |
| 大豆(Glycine max) | GmPeNTL9 | 剪接变体激活抗氧化清除系统 | 盐胁迫 | Liu et al., |
| GmSnRK1.1 | 调节转录因子的活性和稳定性 | 盐胁迫 | Liu et al., |
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