bHLH17 Negatively Regulates Jasmonate-mediated Plant Defense Responses

doi:10.3724/SP.J.1259.2014.00643

Abstract

Abstract: The plant hormone jasmonate (JA) functions as a defense signal to regulate plant defense against insect attack and pathogen infection. It also acts as a developmental signal to regulate plant development and growth, including root growth, stamen development, trichome initiation, and leaf senescence. The JA receptor CORONATINE INSENSITIVE1 (COI1) perceives JA signals for interaction with JASMONATE-ZIM-DOMAIN (JAZ) proteins and subsequent ubiquitination of JAZs, thereby regulating the JA responses. The single mutants of the bHLH subgroup IIId factors (bHLH3, bHLH13, bHLH14 and bHLH17), which interact with JAZs, show no obvious phenotype in resistance to the fungus Botrytis cinerea and defense against the herbivore Spodoptera exigua. However, the quadruple mutant shows enhanced resistance to B. cinerea and defense against S. exigua. We investigated whether the overexpression of bHLH17 in Arabidopsis affected the resistance to B. cinerea and defense against S. exigua. Similar to the JA receptor mutant coi1-1, Arabidopsis bHLH17-overexpressing plants were more susceptible to B. cinerea and S. exigua than the wild type. As compared with the wild type, bHLH17 overexpression attenuated the JA-inducible expression of defense- or wounding-related genes, such as Thi2.1, VSP2, AOS, JAZ1, JAZ9 and JAZ10. Further transient expression analysis revealed that the N-terminus of bHLH17 was essential for its transcriptional repression function. Overexpression of bHLH17 negatively regulates plant defense against B. cinerea and S. exigua.

Key words: bHLH17, COI1, defense, jasmonate

Cuili Wang, Haixia Tian, Jiaojiao Wang, Tiancong Qi, Huang Huang, Chunmei Ren, Daoxin Xie, Susheng Song. bHLH17 Negatively Regulates Jasmonate-mediated Plant Defense Responses[J]. Chinese Bulletin of Botany, 2014, 49(6): 643-652.

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URL: https://www.chinbullbotany.com/EN/10.3724/SP.J.1259.2014.00643

https://www.chinbullbotany.com/EN/Y2014/V49/I6/643

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