Differences in Adaptive
Development of Suberin in Tobacco Root Endothelial Layer under Different Potassium
Levels

doi:10.11983/CBB24191

Abstract

Abstract: INTRODUCTION: The apoplastic barriers differentiated from the root endodermis play an important role in plant stress resistance and nutrient uptake, and the development of suberin lamella has become a hot research topic in recent years.

RATIONALE: Hydroponic experiments with potassium concentration gradients ranging from 0.1 to 4.0 mmol∙L^–1 were conducted, using the cultivated tobacco variety Zhongyan 100 as the experimental material, to explore the effect of different potassium supplies on the endodermal suberization and its physiological and molecular mechanisms.
RESULTS: It was found that low potassium stress (0.1 mmol∙L^–1) significantly enhanced the endodermal suberization: the absolute length of the fully suberized region extended from 0–2 cm in the control to 4–6 cm, and the relative proportion increased from 0–15.0% to 33.2–44.3%. This indicates that suberization is one of the key morphological adaptation mechanisms in tobacco under low potassium stress. Phenotypic analysis showed that under low potassium stress, root elongation increased while plant biomass decreased, and potassium ion content and accumulation in both the aboveground and root parts were reduced. Additionally, the flow rate and potassium ion concentration in the xylem sap decreased, indicating reduced transport efficiency. Endogenous hormone analysis revealed that low potassium stress increased abscisic acid (ABA) content in roots while suppressing ethylene and methyl jasmonate levels, forming a specific hormonal regulatory network. Transcriptome data further supported the molecular basis of suberization development, showing significant upregulation of genes related to suberin synthesis and transport (e.g., CYP86, GPAT, ABCG) and their upstream positive regulatory factors (MYB36, MYB41, MYB92, MYB93).
CONCLUSION: This study is the first to elucidate that low potassium stress regulates the suberization developmental program of tobacco root through ABA-mediated hormonal signaling reprogramming, providing a novel perspective for understanding the adaptation mechanisms of plants to potassium stress.

Fluorescence imaging of transverse sections of endodermis development in tobacco root under three potassium concentrations

Key words: suberization,, potassium,, root endodermis,, hormone

Yunxiang Xu, Liwen Zhang, Peng Wang, Yingchen Gu, Madan Lal Kolhi, Biao Zhang, Yingying Zhu, Haiwei Liu.

Differences in Adaptive Development of Suberin in Tobacco Root Endothelial Layer under Different Potassium Levels

[J]. Chinese Bulletin of Botany, DOI: 10.11983/CBB24191.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB24191

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