天山雪莲SiSAD基因与拟南芥AtFAB2基因转化 烟草的抗寒性分析

doi:10.11983/CBB17129

植物学报 ›› 2018, Vol. 53 ›› Issue (5): 603-611.DOI: 10.11983/CBB17129

天山雪莲SiSAD基因与拟南芥AtFAB2基因转化烟草的抗寒性分析

陈建权, 程晨, 张梦恬, 张向前, 张尧, 王爱英, 祝建波^*()

石河子大学生命科学学院, 农业生物技术重点实验室, 石河子 832003

收稿日期:2017-07-08 接受日期:2017-10-25 出版日期:2018-09-01 发布日期:2018-11-29
通讯作者: 祝建波
作者简介:

作者简介：路安民(图中左), 植物系统分类学家。20世纪60-70年代编著《中国植物志》等, 后从事植物系统发育和进化研究。“七五”以来主持了4项中科院、国家自然科学基金委重大和重点项目。1991年获国务院颁发的有突出贡献科学家荣誉证书。1987年8月-1990年12月担任中科院植物所所长。
基金资助:
转基因重大专项(No.2016ZX08005-004)

Cold-tolerance Analysis of Tobacco Plants Transformed with Saussurea involucrata SiSAD and Arabidopsis thaliana AtFAB2 Gene

Chen Jianquan, Cheng Chen, Zhang Mengtian, Zhang Xiangqian, Zhang Yao, Wang Aiying, Zhu Jianbo^*()

Key Laboratory of Agricultural Biotechnology, College of Life Science, Shihezi University, Shihezi 832003, China

Received:2017-07-08 Accepted:2017-10-25 Online:2018-09-01 Published:2018-11-29
Contact: Zhu Jianbo
About author:
† These authors contributed equally to this paper

摘要/Abstract

摘要： 通过转基因烟草(Nicotiana tabacum)验证天山雪莲(Saussurea involucrata) Δ9硬脂酰-ACP脱饱和酶基因SiSAD与拟南芥(Arabidopsis thaliana)中同源基因AtFAB2的抗寒性功能。利用农杆菌介导法将植物表达载体P_SiSAD:AtFAB2和P_SiSAD:SiSAD导入烟草, 然后将2种转基因和野生型烟草分别置于20°C、10°C、5°C、0°C及-2°C下处理2小时, 检测其相对电导率、丙二醛(MDA)含量、叶绿素荧光参数(F_v/F_m)及脂肪酸含量。将-2°C处理2小时后的植株置于25°C培养1周进行生长恢复实验。结果表明, 生长恢复实验中转SiSAD基因烟草的恢复效果显著优于转AtFAB2基因和野生型烟草。在0°C和-2°C处理2小时后, 转SiSAD、AtFAB2基因型和野生型烟草的相对电导率和丙二醛含量呈现显著递增趋势; 转SiSAD、AtFAB2基因型烟草的F_v/F_m显著高于野生型烟草, 其中, 转SiSAD基因烟草的F_v/F_m显著高于转AtFAB2基因烟草。转AtFAB2基因型和野生型烟草的油酸(C18:1)含量随着温度的降低逐渐升高后降低并在0°C时达到最高值; 而转SiSAD基因型烟草C18:1含量持续升高, 并在-2°C时达到最高值, 其含量分别是转AtFAB2基因型和野生型烟草的1.58倍和1.7倍。以上结果表明, 天山雪莲Δ9硬脂酰-ACP脱饱和酶基因SiSAD与拟南芥中同源基因AtFAB2均可以显著增强非低温驯化烟草的抗寒性, 但是SiSAD基因效果显著优于AtFAB2。

关键词: 低温, 新疆雪莲, SiSAD, AtFAB2, 抗寒性

Abstract: The SiSAD gene in Saussurea involucrata and its homologous gene AtFAB2 in Arabidopsis thaliana have been reported to encode homeologous Δ9 stearoyl-acp desaturases. To investigate the function of these genes in plants’ response to cold stress, we constructed two expression vectors PSiSAD:AtFAB2 and PSiSAD:SiSAD, and were Agrobacterium-infiltrated in tobacco. These two kinds of transgenic plants and wild-type tobacco were treated at 20°C, 10°C, 5°C, 0°C, and -2°C for 2 h and then to determine the relative conductivity, malondialdehyde (MDA) and fatty acid content and chlorophyll fluorescence parameters (F_v/F_m). Furthermore, after -2°C treatment for 2 h, and recovery at 25°C for 1 week, we examined recovery rate of these tobacco plants. The recovery rate of SiSAD transgenic tobacco was much better than AtFAB2 transgenic tobacco and wild type. After treatment at 0°C and -2°C for 2 h, the relative conductivity and MDA content of the SiSAD and AtFAB2 transgenic tobacco and wild-type tobacco showed a significant increasing trend. The F_v/F_m of SiSAD and AtFAB2 transgenic tobacco were significantly higher than wild-type tobacco and the F_v/F_m of SiSAD transgenic tobacco was significantly higher than AtFAB2 transgenic tobacco. The content of oleic acid (C18:1) in AtFAB2 transgenic tobacco and wild type were decreased gradually with decreasing temperature and reached the lowest level at 0°C, whereas the content of C18:1 in SiSAD transgenic tobacco increased and peaked at -2°C; The C18:1 contents in SiSAD transgenic tobacco were at least 1.58 and 1.7 folds when compared to AtFAB2 transgenic tobacco and wild type. These results indicate that SiSAD and AtFAB2 genes can significantly enhance the cold tolerance of a non-cold acclimated tobacco. Moreover, the SiSAD gene plays more important role than AtFAB2 gene in cold tolerance.

Key words: low temperature, Saussurea involucrata, SiSAD, AtFAB2, cold tolerance

陈建权, 程晨, 张梦恬, 张向前, 张尧, 王爱英, 祝建波. 天山雪莲SiSAD基因与拟南芥AtFAB2基因转化烟草的抗寒性分析. 植物学报, 2018, 53(5): 603-611.

Chen Jianquan, Cheng Chen, Zhang Mengtian, Zhang Xiangqian, Zhang Yao, Wang Aiying, Zhu Jianbo. Cold-tolerance Analysis of Tobacco Plants Transformed with Saussurea involucrata SiSAD and Arabidopsis thaliana AtFAB2 Gene. Chinese Bulletin of Botany, 2018, 53(5): 603-611.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB17129

https://www.chinbullbotany.com/CN/Y2018/V53/I5/603

图/表 7

图1 2种植物表达载体结构

Figure 1 The structure of two plant expression vectors

表1 RT-PCR引物

Table 2 Primers of RT-PCR

Primer name	Primer sequence (5'-3')
SAD F	GTTGGAGATATGATCCACGAGGAAGC
SikSAD R	TTCCAGTATATCGGCATAGTCCTT
AtFAB2 F	GCACATGCGTGACATGCTTC
AtFAB2 R	CTGATCGACGGTCAATTGGC
GAPDH F	GTTGCTAGAGTTGCACTTCAGAGAG
GAPDH R	TTCCTGAAGCCGAAAACAGC

图2 转PSiSAD:SiSAD和PSiSAD:AtFAB2重组质粒烟草的PCR鉴定(A) 转PSiSAD:SiSAD重组质粒烟草基因PCR鉴定(M: Marker III DNA分子标量; 1-7: 不同转基因株系; 8: 野生型(阴性对照); 9: PSiSAD:SiSAD质粒(阳性对照); (B) 转PSiSAD:AtFAB2重组质粒烟草基因PCR鉴定(M: Marker III DNA分子标量; 1-10: 不同转基因株系; 11: 野生型(阴性对照); 12: PSiSAD:AtFAB2质粒(阳性对照))

Figure 2 PCR identification of tobacco transferred with PSiSAD:SiSAD and PSiSAD:AtFAB2 recombinant plasmid, respectively (A) PCR identification of tobacco transferred with PSiSAD: SiSAD recombinant plasmid (M: Marker III DNA marker; 1-7: Different transgenic lines; 8: Wild type (negative control); 9: PSiSAD:SiSAD plasmid (positive control)); (B) PCR identification of tobacco transferred with PSiSAD:AtFAB2 recombinant plasmid (M: Marker III DNA marker; 1-10: Different transgenic lines; 11: Wild type (negative control); 12: PSiSAD:SiSAD plasmid (positive control))

图3 转PSiSAD:SiSAD和PSiSAD:AtFAB2重组质粒烟草的RT- PCR鉴定

Figure 3 RT-PCR identification of tobacco transferred with PSiSAD:SiSAD and PSiSAD:AtFAB2 recombinant plasmid, respectively

图4 不同温度处理下野生型和转基因烟草表型(A)-(E) 野生型和转基因烟草在20°C、10°C、5°C、0°C及-2°C各处理2小时; (F) -2°C处理后, 25°C恢复培养1周。s-f: PSiSAD: AtFAB2; s-s: PSiSAD:SiSAD; WT: 野生型

Figure 4 Phenotype of wild-type and transgenic tobacco under different temperatures(A)-(E) Wild-type and transgenic tobacco plants grown at 20°C, 10°C, 5°C, 0°C, and -2°C for 2 hours, respectively; (F) After -2°C treatment recovering in 25°C for one week.s-f: PSiSAD:AtFAB2; s-s: PSiSAD:SiSAD; WT: Wild-type

表2 不同温度下转基因烟草的脂肪酸含量测定

Table 2 The analysis of the content of desaturation products in transgenic tobacco under different temperatures

Temperature (°C)	Plant	Fatty acid (%)
Temperature (°C)	Plant	C16:0	C18:0	C18:1	C18:2	C18:3	Total desaturation products
20	WT	43.70±1.10 a	24.77±0.66 a	14.33±0.94 a	3.27±0.43 a	12.97±1.56 a	16.57
	s-f	42.27±1.11 a	20.89±1.09 b	15.20±0.38 b	2.77±0.92 a	7.37±1.24 b	17.34
	s-s	46.53±0.90 a	20.03±0.32 c	17.23±1.60 c	4.61±1.15 a	7.07±0.93 b	21.91
10	WT	38.99±0.58 a	19.83±0.65 a	15.87±0.92 a	2.53±0.30 a	8.17±1.22 a	17.57
	s-f	42.97±0.62 a	15.87±0.86 a	17.43±0.48 a	2.07±0.12 a	8.19±1.12 a	19.69
	s-s	40.76±0.79 a	15.70±2.80 a	19.40±1.23 c	1.77±0.38 a	6.00±1.11 a	23.17
5	WT	30.30±0.80 a	16.47±0.66 a	17.50±0.44 a	3.70±0.12 a	17.20±2.02 a	19.4
	s-f	25.27±0.66 b	13.83±0.71 b	18.70±1.29 b	1.90±0.17 a	10.20±0.86 b	20.8
	s-s	46.43±0.41 c	12.30±0.67 a	20.70±0.57 b	2.67±0.09 a	6.60±0.68 c	31.97
0	WT	40.53±0.44 a	16.23±0.32 a	17.90±0.76 a	2.20±0.17 a	8.47±0.41 a	22.67
	s-f	47.63±0.47 b	13.67±0.73 b	19.17±1.45 b	2.33±0.27 a	11.97±1.44 a	28.47
	s-s	44.33±0.07 b	12.47±0.45 c	23.50±1.33 a	2.30±0.46 a	11.87±2.07 a	37.67
-2	WT	38.20±1.07 a	15.57±0.76 a	15.63±2.21 a	2.87±0.20 a	10.23±0.72 a	26.73
	s-f	40.20±0.23 b	11.20±1.56 b	16.54±0.49 a	3.13±0.59 a	16.30±1.22 b	31.97
	s-s	53.60±0.47 c	10.30±0.64 b	30.47±0.89 b	2.33±0.48 a	9.83±2.24 a	49.63
Recover treatment	WT	36.30±1.04 a	14.46±0.68 a	14.77±2.11 a	2.46±0.22 a	10.02±0.68 a	25.94
	s-f	41.40±1.03 a	20.05±1.03 b	14.91±0.34 b	2.58±0.85 a	7.19±1.18 b	16.87
	s-s	46.33±0.87 a	20.01±0.30 c	17.14±1.53 c	4.57±1.12 a	6.98±0.90 b	21.23

图5 不同温度处理下野生型和转基因烟草的生理指标(A) 相对电导率; (B) 叶绿素荧光参数(Fv/Fm); (C) 丙二醛(MDA)含量。处理条件: 野生型和转基因烟草在20°C、10°C、5°C、0°C和-2°C各处理2小时, 恢复处理是在-2°C处理2小时后25°C恢复培养1周。不同小写字母代表差异显著(P<0.05)。s-f: PSiSAD:AtFAB2; s-s: PSiSAD:SiSAD; WT: 野生型

Figure 5 The physiological analysis of wild-type and transgenic tobacco plant after different processing temperature (A) Relative conductivity; (B) Maximum efficiency of photosystem II photochemistry (Fv/Fm); (C) Malondialdehyde (MDA) content; Wild-type and transgenic tobacco plants grown at 20°C, 10°C, 5°C, 0°C, and -2°C; recovery treatment: after -2°C treatment for 2 hours recovery in 25°C for 1 week. Different lowercase letters indicate significant differences at P<0.05. s-f: PSiSAD:AtFAB2; s-s: PSiSAD:SiSAD; WT: Wild-type

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天山雪莲SiSAD基因与拟南芥AtFAB2基因转化烟草的抗寒性分析

Cold-tolerance Analysis of Tobacco Plants Transformed with Saussurea involucrata SiSAD and Arabidopsis thaliana AtFAB2 Gene

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天山雪莲SiSAD基因与拟南芥AtFAB2基因转化 烟草的抗寒性分析

Cold-tolerance Analysis of Tobacco Plants Transformed with Saussurea involucrata SiSAD and Arabidopsis thaliana AtFAB2 Gene

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天山雪莲SiSAD基因与拟南芥AtFAB2基因转化烟草的抗寒性分析