病原菌诱导型启动子顺式作用元件及其互作的转录因子

doi:10.3724/SP.J.1259.2013.00219

摘要/Abstract

摘要： 启动子是位于基因5′端上游的一段DNA序列, 负责调控基因的转录。与植物抗病相关基因的启动子区含有能针对病原菌胁迫做出应答的顺式作用元件, 这些顺式作用元件通过与转录因子特异性结合, 进而增强抗病基因的转录表达, 提高植物的抗病性。该文主要综述了病原菌诱导型启动子相关顺式作用元件及与这些元件互作的转录因子, 特别对一类特殊的转录因子——病原菌TAL效应子与植物靶基因启动子之间的相互作用机制进行了阐述, 并对其应用前景进行了展望。

关键词: 顺式作用元件, 诱导型启动子, 病原菌, 抗病性, 转录因子

Abstract: A gene’s promoter is the 5' upstream region of the DNA sequence and it regulates the transcription of a gene. Pathogen-inducible promoters contain cis-acting elements responsive to pathogenic stresses. The cis-acting elements can enhance the transcription of a resistant gene to improve disease resistance in a plant by specifically interacting with transcription factors. This article reviews the pathogen-related cis-acting elements in pathogen-inducible promoters and the transcription factors that interact with these components. It elaborates the mechanism between the transcription activator-like effectors and the target gene promoters in plants. Possible applications and future prospects for these pathogen-responsive cis-acting elements are discussed.

Key words: cis-acting elements, inducible promoter, pathogens, disease resistance, transcription factors

中图分类号:

昝新丽, 高英, 陈玉玲, 赵开军. 病原菌诱导型启动子顺式作用元件及其互作的转录因子. 植物学报, 2013, 48(2): 219-229.

Xinli Zan, Ying Gao, Yuling Chen, Kaijun Zhao. Pathogen-responsive Cis-acting Elements and Their Interactive Transcription Factors. Chinese Bulletin of Botany, 2013, 48(2): 219-229.

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链接本文: https://www.chinbullbotany.com/CN/10.3724/SP.J.1259.2013.00219

https://www.chinbullbotany.com/CN/Y2013/V48/I2/219

参考文献

陈俊, 王宗阳 (2002). 植物MYB类转录因子研究进展. 植物生理与分子生物学学报2, 81–88.
李岩强, 王春连, 赵开军 (2011). 病原菌TAL 效应子与寄主靶基因相互识别的分子密码. 生物工程学报 8, 1132?1141.
刘蕾, 杜海, 唐晓凤, 吴燕民, 黄玉碧, 唐益雄 (2008). MYB 转录因子在植物抗逆胁迫中的作用及其分子机理. 遗传HEREDITAS(Beijing) 30(10), 1265–1271.
万小荣, 李玲 (2002). 植物的MYB蛋白. 植物生理学通讯38(2), 165–170.
吴坤陆 (2005)．WRKY基因家族的进化及水稻WRKY基因表达谱的研究. 博士论文. 杭州：浙江大学. pp. 16–21.
吴雪峰 (2009). 诱导型启动子BjC-P的功能分析. 博士论文. 北京: 中国农业科学院研究生院. pp. 22?33.
张海文，谢丙炎，卢向阳，杨宇红，陈琪，黄荣峰 (2004). 拟南芥防卫基因PDF1.2 启动子中GCC 盒是应答茉莉素反应必要的顺式作用元件. 科学通报 23, 2444–2448.
赵开军, 李岩强, 王春连, 高英 (2011). 植物天然免疫性研究进展及其对作物抗病育种的可能影响. 作物学报6: 935–942.
朱玉贤，李毅 (2002). 现代分子生物学(第2版). 北京：高等教育出版社. pp. 71–77.
Boch J, Bonas U (2010). Xanthomonas AvrBs3 family-type III effectors: discovery and function. Annu Reveal Phytopathol 48, 419?436.
Boch J, Scholze H, Schornack S, Landgraf A, Hahn S, Kay S, Lahaye T, Nickstadt A, Bonas U (2009). Breaking the code of DNA binding specificity of TAL-type III effectors. Science 326, 1509–1512.
Bonas U, Stall R E, Staskawicz B (1989). Genetic and Structural characterization of the avirulence gene avrBs3 from Xanthomonas campestris pv. vesicatoria. Mol Gen Genet 218, 127–136.
Brown RL, Kazan K, McGrath KC, Maclean DJ, Manners JM ( 2003). A Role for the GCC-Box in Jasmonate-Mediated Activation of the PDF1.2 Gene of Arabidopsis. Plant Physiology 132, 1020–1032.
Buttner M, Singh KB (1997). Arabidopsis thaliana ethylene-responsive element binding protein (AtEBP), an ethylene-inducible GCC box DNA-binding protein interacts with an ocs element binding protein. Proc Natl Acad Sci USA 94, 5961–5966.
Cai M, Qiu D, Yuan T, Ding X, Li H, Duan L, Xu C, Li X, Wang S (2008). Identification of novel pathogen-responsive cis-elements and their binding proteins in the promoter of OsWRKY13,a gene regulating rice disease resistance. Plant Cell Environ 31, 86–96.
Chujo T, Sugioka N, Masuda Y, Shibuya N, Takemura T, Okada K, Nojiri H, Yamane H (2009). Promoter analysis of the elicitor-induced WRKY gene OsWRKY53,which is involved in defense responses in rice. Biosci Biotechnol Biochem 73, 1901–1904.
Chye, M.L., Zhao, K.J., He, Z.M., Ramalingam, S., Fung, K.L (2005). An agglutinating chitinase with two chitin-binding domains confers fungal protection in transgenic potato. Planta 220, 717–730.
Conrads-Strauch JHK, Bonas U (1992). Race-specificity of plant resistance to bacterial spot disease determined by repetitive motifs in a bacterial avirulence protein. Nature 356, 172–174.
da Costae Silva O, Klein L, Schmelzer E, Trezzini GF, Hahlbrock K (1993). BPF-1, a pathogen-induced DNA-binding protein involved in the plant defense response. Plant J 4, 125–135.
Deng D, Yan CY, Pan XJ, Mahfouz M, Wang JW, Zhu JK, Shi YG, Yan N (2012). Structural Basis for Sequence-Specific Recognition of DNA by TAL Effectors. Science 1, 1–9.
Dong J, Chen C, Chen Z (2003)．Expression profiles of the Arabidopsis WRKY gene superfamily during plant defense response C. Plant Mol Biol 5I, 21–37.
Dong JX, Chen CH, Chen ZX (2003). Expression profiles of the Arabidopsis WRKY gene suerfamily during plant defense response. Plant Mol Biol 51(1), 21–37.
Dr?ge-Laser W, Kaiser A, Lindsay WP, Halkier BA, Loake GJ, Doerner P, Dixon RA, Lamb C (1997). Rapid stimulation of a soybean protein-serine kinase that phosphorylates a novel bZIP DNA-binding protein, G/HBF-1, during the induction of early transcription-dependent defenses. EMBO J 16, 726–738.
Ellis JG, Tokuhisa JG, Llewellyn DJ, Bouchez D, Singh K, Dennis ES, Peacock WS (1993). Does the ocs-element occur as a functional component of the promoters of plant genes?. Plant J 4:433–443.
Eulgem T, Rushton PJ, Schmelzer E, Hahlbrock K, Somssich IE (1999). Early nuclear events in plant defense signalling: rapid gene activation by WRKY transcription factors. EMBO J 18(17), 4689–4699.
Faktor O, Kooter JM, Loake GJ, Dixon RA, Lamb CJ (1997a). Differential utilization of regulatory cis-elements for stress-induced and tissue-specific activity of a French bean chalcone synthase promoter. Plant Sci 124, 175–182.
Faktor O, Loake G, Dixon RA, Lamb CJ (1997b). The G-box and H-box in a 39 bp region of a French bean chalcone synthase promoter constitutes a tissue-specific regulatory element. Plant J 11, 1105–1113.
Feldbrugge M, Sprenger M, Hahlbrock K, Weisshaar B (1997). PcMYB1 a novel plant protein containing a DNA-binding domain with one MYB repeat, interacts in vivo with a light-regulatory promoter unit. Plant J 11, 1079–1093.
Foster R, Izawa T, Chua NH (1994). Plant bZIP proteins gather at ACGT elements. FASEB J 8 :192–200.
Fukuda Y (1997). Interaction of tobacco nuclear protein with an elicitor-responsive element in the promoter of a basic class I chitinase gene. Plant Mol Biol 34(1), 81–87.
Fukuda Y, Shinshi H (1994). Characterization of a novel cis—acting element that is responsive to a fungal elicitor in the promoter of a tobacco class I chitinase gene．Plant Mol Biol 24(3), 485– 493.
Fung KL, Zhao KJ, He ZM, Chye ML (2002). Tobacco-expressed Brassica juncea chitinase BjCHI1 shows antifungal activity in vitro. Plant Mol Biol 50, 283–294.
Grimmig B and Matern U (1997). Structure of the parsley caffeoyl-CoA0-methyltransferase gene, harbouring a novel elicitor responsive cis-acting element. Plant Molecular Biology 33: 323–341.
Herbers K, Conrads-Strauch J, Bonas U (1992). Race-specificity of plant resistance to bacterial spot disease determined by repetitive motifs in a bacterial avirulence protein. Nature 6365, 172?174.
Johnson CS, Kolevski B, Smyth DR (2002). TRANSPARENTTESTA GLABRA2, a trichome and seed coat development gene of Arabidopsis, encodes a WRKY transcription factor. Plant Cell 14(6), 1359–1375.
Joshi CP (1987). An inspection of the domain between Putative TATA box and translation start site in 79 plant genes. Nucleic Acids Res 15, 6643–6653.
Kasuga M, Liu Q, Miura S, Yamaguchi-Shinozaki K, Shinozaki K (1999). Improving plant drought, salt and freezing tolerance by gene transfer of a single stress-inducible transcription factor. Nat Biotechnol 17, 87–291.
Kawagoe Y, Murai N (1996). A novel basic region/helix-loop-helix protein binds to a G-box motif CACGTG of the bean storage protein beta-phaseolin gene. Plant Sci 116, 47–57.
Kay S, Hahn S, Marois E, Hause G, Bonas U (2007). A bacterial effector acts as a plant transcription factor and induces a cell size regulator. Science 318, 648–651.
Kim SR, Choi JL, Costa MA, An G (1992). Identification of G-box sequence as an essential element for methyl jasmonate response of potato proteinase inhibitor II promoter. Plant Physiol 99, 627–631.
Kirsch C, Takamiya-Wik M, Schmelzer E, Hahlbrock K, Somssich IE (2000). A novel regulatory element involved in rapid activation of parsley ELI7 gene family members by fungal elicitor or pathogen infection. Mol Plant Pathol 4, 243–251.
Lam E, Benfey PN, Gilmartin PM, Fang RX, Chua NH (1989). Site specific mutations alter in vitro factor binding and change promoter expression pattern in transgenic plants. Proc Natl Acad Sci USA 86: 7890–7894.
Li J, Brader G, Palva ET (2004). The WRKY70 transcription factor: a node of convergence for jasmonate-mediated and salicylate-mediated signals in plant defense. Plant Cell 16(2), 319–331.
Li T, Huang S, Jiang WZ, Wright D, Spalding MH, Weeks DP, Yang B (2011). TAL nucleases (TALNs): hybrid proteins composed of TAL effectors and FokI DNA-cleavage domain. Nucleic Acids Research 1, 359–372.
Lippok B, Birkenbihl RP, Rivory G, Brummer J, Schmelzer E, Logemann E, Somssich IE (2007). Expression of AtWRKY33 encoding a pathogen- or PAMP-responsive WRKY transcription factor is regulated by a composite DNA motif containing W box elements. Mol Plant Microbe Interact 20, 420–429.
Lois R, Dietrich A, Hahlbrock K, Schulz W (1989). A phenylalanine ammonia-lyase gene from parsley: structure, regulation and identification of elicitor and light responsive cis-acting elements. EMBO J 8, 1641–1648.
Maleck K, Levine A, Eulgem T, Morgan A, Schmid J, Lawton KA, Dangl JL, Dietrich RA (2000). The transcriptome of Arabidopsis thaliana during systemic acquired resistance. Nat Genet 26(4), 403–410.
Meier I, Hahlbrock K, Somssich IE (1991). Elicitor-inducible and constitutive in vivo DNA footprints indicate novel cis-acting elements in the promoter of a parsley gene encoding pathogenesis-related protein 1. Plant Cell 3, 309–315.
Menke FLH, Champion A, Kijne JW, Memelink J (1999). A novel jasmonate-and elicitor-responsive element in the periwinkle secondary metabolite biosynthetic gene Str interacts with a jasmonate and elicitor-inducible AP2-domain transcription factor, 0RCA2. EMBO J 18:4455–4463.
Menkens AE, Schindler U, Cashmore AR (1995). The G-box: a ubiquitous regulatory DNA element in plants bound by the GBF family of bZIP proteins. Trends Biochem Sci 20, 506–510.
Moreno AB, Penas G, Rufat M, Bravo JM, Estopa M, Messeguer J, San Segundo B (2005). Pathogen-induced production of the antifungal AFP protein from Aspergillus giganteus confers resistance to the blast fungus Magnaporthe grisea in transgenic rice. Mol Plant Microbe Interact 18, 960–972.
Moscou MJ, Bogdanove AJ (2009). A simple cipher governs DNA recognition by TAL effectors. Science 5959, 1501.
Odell JT , Nagy F, Chua NH (1985). Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter. Nature 313, 810–812.
Ohme-Takagi M, Shinshi H (1995). Ethylene-inducible DNA binding proteins that interact with an ethylene-responsive element. Plant Cell 7, 173–182.
Figueroa P, Browse J (2011). The Arabidopsis JAZ2 Promoter Contains a G-Box and Thymidine-Rich Module that are Necessary and Sufficient for Jasmonate-Dependent Activation by MYC Transcription Factors and Repression by JAZ Proteins. Plant Cell Physiol 2, 330–343.
Pandey SP, Somssich IE (2009). The role of WRKY transcription factors in plant immunity. Plant Physiology 150, 1648-1655.
Raffaele S, Rivas S, Roby D (2006). An essential role for salicylic acid in AtMYB30 mediated control of the hypersensitive cell death program in Arabidopsis. FEBS Lett 580(14), 3498–3504.
Raventors D, Jensen AB, Rask MB, Casacuberta JM, Mundy J, Segundo BS (1995). A 20bp cis-acting element is both necessary and sufficient to mediate elicitor response of a maizes PRms gene. The Plant Journal 7(1), 147–155.
Robatzek S, Somssich IE (2001). A new member of the ArabidopsisWRKY transcription factor family, AtWRKY6, is associated with both senescence-and defence-related processes. Plant J 28(2), 123–133.
R?mer P, Hahn S, Jordan T, Strauss T, Bonas U, Lahaye T (2007). Plant pathogen recognition mediated by promoter activation of the pepper Bs3 resistance gene. Science 318: 645–648.
R?mer P, Recht S, Lahaye T (2009b). A single plant resistance gene promoter engineered to recognize multiple TAL effectors from disparate pathogens. Proc Natl Acad Sci USA 106(48), 20526?20531.
R?mer P, Strauss T, Hahn S, Scholze H, Morbitzer R, Grau J, Bonas U, Lahaye T (2009a). Recognition of AvrBs3-like proteins is mediated by specific binding to promoters of matching pepper Bs3 alleles. Plant Physiol 4, 1697?1712.
Rushton PJ, Macdonald H, Huttly AK, Lazarus CM, Hooley R (1995). Members of a new family of DNA-binding proteins bind to a conservative cis-element in the promoters of -Amy2 genes. Plant Mol Biol 29, 691–702.
Rushton PJ, Somssich IE (1998). Transcriptional control of plant genes responsive to pathogens. Plant Biology 1, 311–315.
Rushton PJ, Torres JT, Parniske M, Wernert P, Hahibrock K, Somssich IE (1996). Interaction of elicitor induced DNA—binding proteins with elicitor response elements in the promoters of parsley PR1 genes．EMBO J 5(20), 5690–5700.
Rushton PJ, Reinst?dler A, Lipka V, Lippok B, Somssich IE (2002). Synthetic plant Promoters containing defined regulatory elements provide novel insights into pathogen and wound-induced signaling. Plant Cell 14(4), 749–762.
Stockinger EJ, Gilmour SJ, Thomashow MF (1997). Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit. Proc Natl Acad Sci USA 94, 1035–1040.
Sugio A, Yang B, Zhu T, White FF (2007). Two type III effector genes of Xanthomonas oryzae pv. oryzae control the induction of the host genes OsTFIIAγ1 and OsTFX1 during bacterial blight of rice. Proc Natl Acad Sci USA 104, 10720–10725.
Sun C, Palmqvist S, Olsson H, Borén M, Ahlandsberg S, Jansson C (2003). A novel WRKY transcription factor, SUSIBA2, participates in sugar signaling in barley by binding to the sugar-reponsive elements of the iso1 promoter. Plant Cell 15(9), 2076–2092.
Thompson MA, Ramsay RG (1995). Myb: an old oncoprotein with new roles. Bioessays 4, 341–350.
Turck F, Zhou A, Somssich IE (2004). Stimulus-dependent, promoter-specific binding of transcription factor WRKY1 to its native promoter and the defense-related gene PcPR1-1 in parsley. Plant Cell 16(10), 2573–2585.
Wang Z, Yang P, Fan B, Chen Z (1998). An oligo selection procedure for identification of sequence-specific DNA-binding activities associated with plant defense. Plant J 16(4), 515–522.
Yang B, Sugio A, White FF (2005). Avoidance of host recognition by alterations in the repetitive and C-terminal regions of AvrXa7, a type III effector of Xanthomonas oryzae pv. oryzae. Mol Plant-Microbe Interact 18, 142–149.
Yang B, Sugio A, White FF (2006). Os8N3 is a host disease-susceptibility gene for bacterial blight of rice. Proc Natl Acad Sci USA 103, 10503–10508.
Yang Y, Klessig DF (1996). Isolation and characterization of a tobacco mosaic virus-inducible myb oncogene homolog from tobacco. PNAS 93(25), 14972–14977.
Yang Y, Shah J, Klessig DF (1997). Signal perception and transduction in plant defense responses. Genes Dev 11, 1621–1639.
Yu DQ, Chen CH, Chen ZX (2001). Evidence for an important role of WRKY DNA binding proteins in the regulation of NPR1 gene expression. Plant Cell 13(7), 1527–1540.
Yu D, Chen C, Chen Z (2001). Evidence of important role of WRKY DNA binding proteins in the regulation of NPRI gene expression. Plant Cell 13, 1527–1539.
Zhao KJ, Chye ML (1999). Methyl jasmonate induces expression of a novel Brassica juncea chitinase with two chitin-binding domains. Plant Mol Biol 40, 1009–1018.
Zhou J, Tang X, Martin GB (1997). The Pto kinase conferring resistance to tomato bacterial speck disease interacts with proteins that bind a cis-element of pathogenesis-related genes. EMBO J 16, 3207–3218.

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