茶树花挥发物对叶蝉三棒缨小蜂的引诱增强效应

doi:10.11983/CBB21078

植物学报 ›› 2021, Vol. 56 ›› Issue (5): 559-572.DOI: 10.11983/CBB21078

茶树花挥发物对叶蝉三棒缨小蜂的引诱增强效应

穆丹¹, 岂泽华¹, 李沁¹^,², 梁可欣¹, 华绍贵¹, 朱星雨¹, 焦梦婕¹, 饶玉春²^,^*(), 孙廷哲¹^,^*()

¹安庆师范大学生命科学学院, 皖西南生物多样性研究与利用安徽省重点实验室, 安庆 246133
²浙江师范大学化学与生命科学学院, 金华 321004

收稿日期:2021-05-08 接受日期:2021-08-09 出版日期:2021-09-01 发布日期:2021-08-31
通讯作者: 饶玉春,孙廷哲
作者简介:confucian007@126.com
* E-mail: ryc@zjnu.cn;
基金资助:
国家自然科学基金(31971185);国家自然科学基金(31800316);安徽省高校优秀青年人才支持计划重点项目(gxyqZD2020031);一般项目(gxyq2018034);安徽省教育厅自然基金重点项目(KJ2017A359)

Enhanced Attraction of Mymarids (Stethynium empoascae) by Volatiles from Tea Flowers

Dan Mu¹, Zehua Qi¹, Qin Li¹^,², Kexin Liang¹, Shaogui Hua¹, Xingyu Zhu¹, Mengjie Jiao¹, Yuchun Rao²^,^*(), Tingzhe Sun¹^,^*()

¹The Province Key Laboratory of the Biodiversity Study and Ecology Conservation in Southwest Anhui, School of Life Scien¬ces, Anqing Normal University, Anqing 246133, China
²School of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China

Received:2021-05-08 Accepted:2021-08-09 Online:2021-09-01 Published:2021-08-31
Contact: Yuchun Rao,Tingzhe Sun

摘要/Abstract

摘要： 茶树(Camellia sinensis)是我国重要的经济作物。小贯小绿叶蝉(Empoasca onukii)是为害茶树的主要害虫之一。叶蝉三棒缨小蜂(Stethynium empoascae)是小贯小绿叶蝉的主要卵寄生蜂, 但茶树花对叶蝉三棒缨小蜂的引诱机制尚不明确。基于析因设计的方差分析结果显示, 茶树花可引诱天敌叶蝉三棒缨小蜂, 显著缩短其寄生行为的搜寻时间。茶树花可显著增强叶蝉为害茶梢对缨小蜂的吸引作用, 而对健康茶梢的增强效应不显著。基于气相色谱-质谱(GC-MS)的结果显示, 健康茶梢中的挥发物种类和相对含量较少, 而叶蝉为害茶梢和茶树花中挥发物种类和相对含量显著增多。偏最小二乘法判别分析(PLS-DA)结果显示, 茶树花挥发物具有明显的聚类特征。变量重要性投影结果表明, 17种茶树花挥发物在判别分析中可能起重要作用。行为测定结果显示, α-水芹烯、顺式氧化芳樟醇、反式氧化芳樟醇、苯甲醛和苯乙酮对叶蝉三棒缨小蜂具有显著的引诱效果。研究初步阐明了茶树花通过释放挥发物引诱叶蝉三棒缨小蜂的机制, 为制定叶蝉的生物防治策略提供了理论依据。

关键词: 小贯小绿叶蝉, 叶蝉三棒缨小蜂, 茶树, 花挥发物, 偏最小二乘法判别分析(PLS-DA), 气相色谱-质谱(GC-MS), 生物防治

Abstract: The tea plant (Camellia sinensis) is an important economic crop in China. Tea green leafhopper (Empoasca onukii) is the most damaging pest of tea plants and mymarid (Stethynium empoascae) has been classified as its egg parasitoid. However, the mechanism underlying the olfactory attraction of mymarids by tea flowers is still elusive. By factorial design, we showed that tea flowers could attract mymarids which are natural enemies of leafhopper and accelerated the parasitic behavior of mymarid. Tea flower specifically decreased the search time of parasitic behavior of mymarid with tea shoots infested by leafhoppers. However, the boosting effect of tea flower was lost in healthy tea shoots. Compared with health tea shoots, the types and relative contents of volatiles were dramatically increased in infested tea shoots using gas chromatography-mass spectrometry (GC-MS). We classified the volatile expression patterns of healthy tea shoots, infested tea shoots and tea flowers using partial least squares discrimination analysis (PLS-DA). Based on variable importance for the projection (VIP), and identified 17 tea flower volatiles which could potentially discriminate the patterns of volatiles in the three tissues. Olfactometer bioassay showed that α-phellandrene, cis-linaloloxide, trans-linaloloxide, benzaldehyde and acetophenone significantly attract mymarids. Our work has preliminarily demonstrated the defense mechanism mediated by tea flowers and provides novel clues for biological control of tea green leafhopper management.

Key words: tea green leafhopper, mymarid, tea plant, flower volatile, partial least squares discrimination analysis (PLS-DA), gas chromatography-mass spectrometry (GC-MS), biological control

穆丹, 岂泽华, 李沁, 梁可欣, 华绍贵, 朱星雨, 焦梦婕, 饶玉春, 孙廷哲. 茶树花挥发物对叶蝉三棒缨小蜂的引诱增强效应. 植物学报, 2021, 56(5): 559-572.

Dan Mu, Zehua Qi, Qin Li, Kexin Liang, Shaogui Hua, Xingyu Zhu, Mengjie Jiao, Yuchun Rao, Tingzhe Sun. Enhanced Attraction of Mymarids (Stethynium empoascae) by Volatiles from Tea Flowers. Chinese Bulletin of Botany, 2021, 56(5): 559-572.

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB21078

https://www.chinbullbotany.com/CN/Y2021/V56/I5/559

图/表 11

参考文献 58

[1]	边磊 (2014). 基于远程寄主定位机理的假眼小绿叶蝉化学生态和物理调控. 博士论文. 北京: 中国农业科学院. pp. 28-46.
[2]	陈璇, 胡福良 (2009). 蜜蜂花粉采集行为的调控机制. 昆虫知识 46, 490-494.
[3]	董燕梅, 张文颖, 凌正一, 李靖锐, 白红彤, 李慧, 石雷 (2020). 转录因子调控植物萜类化合物生物合成研究进展. 植物学报 55, 340-350. DOI
[4]	戈林泉, 胡中卫, 吴进才 (2013). 大豆、玉米与水稻配置对稻田寄生蜂的影响. 应用昆虫学报 50, 921-927.
[5]	韩宝瑜, 林金丽, 周孝贵, 章金明 (2009). 假眼小绿叶蝉卵及卵寄生蜂缨小蜂形态观察和寄生率考评. 安徽农业大学学报 36, 13-17.
[6]	韩善捷, 潘铖, 韩宝瑜 (2016). 假眼小绿叶蝉为害致茶梢挥发物变化及其引诱微小裂骨缨小蜂效应. 中国生物防治学报 32, 142-148.
[7]	李飞, 杨丹, 郑姣莉, 姚经武, 朱志刚, 黄大野, 曹春霞 (2020). 中国茶园主要害虫生物防治研究进展. 湖北农业科学 59(10), 5-9, 22.
[8]	李秋玲 (2018). 班氏跳小蜂气味结合蛋白OBPs的结合特性分析. 硕士论文. 武汉: 华中农业大学. pp. 25-28.
[9]	林金丽 (2010). 茶树-假眼小绿叶蝉-缨小蜂间化学和色彩通讯机理研究. 硕士论文. 扬州: 扬州大学. pp. 7-12.
[10]	林郑和, 钟秋生, 陈常颂, 陈志辉, 游小妹 (2015). 不同香型茶树鲜叶挥发性组分与β-葡萄糖苷酶的相关性分析. 植物学报 50, 713-720.
[11]	刘丰静, 冉伟, 李喜旺, 汪素琴, 孙晓玲 (2020). 小贯小绿叶蝉在5个茶树品种(系)上的蜜露排泄量与茶树叶片结构比较. 茶叶科学 40, 625-631.
[12]	孟召娜, 边磊, 罗宗秀, 李兆群, 辛肇军, 蔡晓明 (2018). 全国主产茶区茶树小绿叶蝉种类鉴定及分析. 应用昆虫学报 55, 514-526.
[13]	穆丹 (2011). 茶树挥发性信息素调控假眼小绿叶蝉及叶蝉三棒缨小蜂行为的功效. 博士论文. 北京: 中国农业科学院. pp. 12-62.
[14]	聂晓培 (2017). 班氏跳小蜂寄主定位的嗅觉机制. 硕士论文. 武汉: 华中农业大学. pp. 41-47.
[15]	潘铖, 林金丽, 韩宝瑜 (2016). 茶梢信息物引诱叶蝉三棒缨小蜂效应的检测. 生态学报 36, 3785-3795.
[16]	吴国火, 崔林, 王梦馨, 李红亮, 韩宝瑜 (2020). 茶树花香气及茶叶气味对中华蜜蜂的引诱效应. 生态学报 40, 4024-4031.
[17]	张云宣 (2018). 挥发性物质介导的稻纵卷叶螟姬小蜂搜寻与定位寄主行为机制初步研究. 硕士论文. 南宁: 广西大学. pp. 38-42.
[18]	赵冬香, 陈宗懋, 程家安 (2000). 茶小绿叶蝉优势种的归属. 茶叶科学 20, 101-104.
[19]	左照江, 张汝民, 高岩 (2009). 植物间挥发物信号的研究进展. 植物学报 44, 245-252. DOI
[20]	Aartsma Y, Leroy B, Van Der Werf W, Dicke M, Poelman EH, Bianchi FJJA (2019). Intraspecific variation in herbivore-induced plant volatiles influences the spatial range of plant-parasitoid interactions. Oikos 128, 77-86. DOI URL
[21]	Boachon B, Junker RR, Miesch L, Bassard JE, Hofer R, Caillieaudeaux R, Seidel DE, Lesot A, Heinrich C, Ginglinger JF, Allouche L, Vincent B, Wahyuni DSC, Paetz C, Beran F, Miesch M, Schneider B, Leiss K, Werck-Reichhart D (2015). CYP76C1 (Cytochrome P450)-mediated linalool metabolism and the formation of volatile and soluble linalool oxides in Arabidopsis flowers. Plant Cell 27, 2972-2990.
[22]	Bouwmeester H, Schuurink RC, Bleeker PM, Schiestl F (2019). The role of volatiles in plant communication. Plant J 100, 892-907. DOI
[23]	Cao H (2013). Polysaccharides from Chinese tea: recent advance on bioactivity and function. Int J Biol Macromol 62, 76-79. DOI URL
[24]	Chen D, Chen GJ, Sun Y, Zeng XX, Ye H (2020). Physiological genetics, chemical composition, health benefits and toxicology of tea ( Camellia sinensis L.) flower: a review. Food Res Int 137, 109584. DOI PMID
[25]	Chen GJ, Yuan QX, Saeeduddin M, Ou SY, Zeng XX, Ye H (2016). Recent advances in tea polysaccharides: extraction, purification, physicochemical characterization and bioactivities. Carbohydr Polym 153, 663-678. DOI URL
[26]	D'Alessandro M, Brunner V, Von Mérey G, Turlings TCJ (2009). Strong attraction of the parasitoid Cotesia marginiventris towards minor volatile compounds of maize. J Chem Ecol 35, 999-1008. DOI PMID
[27]	Desurmont GA, Von Arx M, Turlings TCJ, Schiestl FP (2020). Floral odors can interfere with the foraging behavior of parasitoids searching for hosts. Front Ecol Evol 8, 148. DOI URL
[28]	Fu JY, Han BY, Xiao Q (2014). Mitochondrial COI and 16sRNA evidence for a single species hypothesis of E. vitis, J. formosana and E. onukii in East Asia. PLoS One 9, e115259. DOI URL
[29]	Gorden NLS, Adler LS (2018). Consequences of multiple flower-insect interactions for subsequent plant-insect interactions and plant reproduction. Am J Bot 105, 1835-1846. DOI URL
[30]	Han BY, Chen ZM (2002a). Composition of the volatiles from intact and mechanically pierced tea aphid-tea shoot complexes and their attraction to natural enemies of the tea aphid. J Agric Food Chem 50, 2571-2575. DOI URL
[31]	Han BY, Chen ZM (2002b). Composition of the volatiles from intact and tea aphid-damaged tea shoots and their allurement to several natural enemies of the tea aphid. J Appl Entomol 126, 497-500. DOI URL
[32]	Huang MS, Sanchez-Moreiras AM, Abel C, Sohrabi R, Lee S, Gershenzon J, Tholl D (2012). The major volatile organic compound emitted from Arabidopsis thaliana flowers, the sesquiterpene (E)-β-caryophyllene, is a defense against a bacterial pathogen. New Phytol 193, 997-1008. DOI URL
[33]	Ishiwari H, Suzuki T, Maeda T (2007). Essential compounds in herbivore-induced plant volatiles that attract the predatory mite Neoseiulus womersleyi. J Chem Ecol 33, 1670-1681. PMID
[34]	Joshi R, Poonam, Saini R, Guleria S, Babu GDK, Kumari M, Gulati A (2011). Characterization of volatile components of tea flowers ( Camellia sinensis) growing in Kangra by GC/MS. Nat Prod Commun 6, 1155-1158.
[35]	Lee LC, Liong CY, Jemain AA (2018). Partial least squares- discriminant analysis (PLS-DA) for classification of high- dimensional (HD) data: a review of contemporary practice strategies and knowledge gaps. Analyst 143, 3526-3539. DOI URL
[36]	Liu FL, Zhang XW, Chai JP, Yang DR (2006). Pollen phenolics and regulation of pollen foraging in honeybee colony. Behav Ecol Sociobiol 59, 582-588. DOI URL
[37]	Maffei ME, Gertsch J, Appendino G (2011). Plant volatiles: production, function and pharmacology. Nat Prod Rep 28, 1359-1380. DOI URL
[38]	McCormick AC, Unsicker SB, Gershenzon J (2012). The specificity of herbivore-induced plant volatiles in attracting herbivore enemies. Trends Plant Sci 17, 303-310. DOI PMID
[39]	Mu D, Cui L, Ge J, Wang MX, Liu LF, Yu XP, Zhang QH, Han BY (2012). Behavioral responses for evaluating the attractiveness of specific tea shoot volatiles to the tea green leafhopper, Empoaca vitis. Insect Sci 19, 229-238. DOI URL
[40]	Poveda K, Steffan-Dewenter I, Scheu S, Tscharntke T (2003). Effects of below- and above-ground herbivores on plant growth, flower visitation and seed set. Oecologia 135, 601-605. PMID
[41]	Qin DZ, Zhang L, Xiao Q, Dietrich C, Matsumura M (2015). Clarification of the identity of the tea green leafhopper based on morphological comparison between Chinese and Japanese Specimens. PLoS One, 10, e0139202. DOI URL
[42]	Rodriguez-Saona CR, Byers JA, Schiffhauer D (2012). Effect of trap color and height on captures of blunt-nosed and sharp-nosed leafhoppers (Hemiptera: Cicadellidae) and non-target arthropods in cranberry bogs. Crop Prot 40, 132-144. DOI URL
[43]	Rohrig E, Sivinski J, Teal P, Stuhl C, Aluja M (2008). A floral-derived compound attractive to the tephritid fruit fly parasitoid Diachasmimorpha longicaudata (Hymenoptera: Braconidae). J Chem Ecol 34, 549-557. DOI PMID
[44]	Sanou A, Traoré F, Ba MN, Dabiré-Binso CL, Pittendrigh BR, Sanon A (2019). Effects of volatiles from Clavigralla tomentosicollis Stål. (Hemiptera: Coreidae) adults on the host location behavior of the egg parasitoid Gryon fulviventre (Crawford) (Hymenoptera: Scelionidae). Int J Insect Sci 11, 1-7.
[45]	Schiestl FP (2015). Ecology and evolution of floral volatile-mediated information transfer in plants. New Phytol 206, 571-577. DOI PMID
[46]	Schnee C, Kollner TG, Held M, Turlings TCJ, Gershenzon J, Degenhardt J (2006). The products of a single maize sesquiterpene synthase form a volatile defense signal that attracts natural enemies of maize herbivores. Proc Natl Acad Sci USA 103, 1129-1134. DOI URL
[47]	Sharma R, Rana A, Kumar S (2020). Phytochemical investigation and bioactivity studies of flowers obtained from different cultivars of Camellia sinensis plant. Nat Prod Res doi: 10.1080/14786419.2020.1844696. DOI
[48]	Simpson M, Gurr GM, Simmons AT, Wratten SD, James DG, Leeson G, Nicol HI (2011). Insect attraction to synthetic herbivore-induced plant volatile-treated field crops. Agr Forest Entomol 13, 45-57. DOI URL
[49]	Takemoto H, Takabayashi JJ (2015). Parasitic wasps Aphidius ervi are more attracted to a blend of host-induced plant volatiles than to the independent compounds. J Chem Ecol 41, 801-807. DOI URL
[50]	Tan XL, Hu NN, Zhang F, Ramirez-Romero R, Desneux N, Wang S, Ge F (2016). Mixed release of two parasitoids and a polyphagous ladybird as a potential strategy to control the tobacco whitefly Bemisia tabaci. Sci Rep 6, 28245. DOI URL
[51]	Tang GY, Meng X, Gan RY, Zhao CN, Liu Q, Feng YB, Li S, Wei XL, Atanasov AG, Corke H, Li HB (2019). Health functions and related molecular mechanisms of tea components: an update review. Int J Mol Sci 20, 6196. DOI URL
[52]	Turlings TCJ, Erb M (2018). Tritrophic interactions mediated by herbivore-induced plant volatiles: mechanisms, ecological relevance, and application potential. Annu Rev Entomol 63, 433-452. DOI PMID
[53]	Wang P, Lou YG (2013). Screening and field evaluation of synthetic plant volatiles as attractants for Anagrus nilaparvatae Pang et Wang, an egg parasitoid of rice planthoppers. Chin J Appl Entomol 50, 431-440.
[54]	Wang ZZ, Liu YQ, Shi M, Huang JH, Chen XX (2019). Parasitoid wasps as effective biological control agents. J Integ Agric 18, 705-715. DOI URL
[55]	Wei JN, Kang L (2011). Roles of (Z)-3-hexenol in plant- insect interactions. Plant Signal Behav 6, 369-371. DOI URL
[56]	Xiu CL, Zhang W, Xu B, Wyckhuys KAG, Cai XM, Su HS, Lu YH (2019). Volatiles from aphid-infested plants attract adults of the multicolored Asian lady beetle Harmonia axyridis. Biol Control 129, 1-11. DOI URL
[57]	Ye J, Zhang LL, Zhang X, Wu XJ, Fang RX (2021). Plant defense networks against insect-borne pathogens. Trends Plant Sci 26, 272-287. DOI URL
[58]	Zhao MY, Wang L, Wang JM, Jin JY, Zhang N, Lei L, Gao T, Jing TT, Zhang SR, Wu Y, Wu B, Hu YQ, Wan XC, Schwab W, Song CK (2020). Induction of priming by cold stress via inducible volatile cues in neighboring tea plants. J Integr Plant Biol 62, 1461-1468. DOI URL

Factor	Level A	Level B
Infestation	Healthy tea shoots (-)	Infested tea shoots (+)
Tea flower	Absent (-)	Present (+)
Mating status	Virgin mymarid (-)	Mated mymarid (+)

Factor	Level A	Level B
Infestation	Healthy tea shoots (-)	Infested tea shoots (+)
Tea flower	Absent (-)	Present (+)
Mating status	Virgin mymarid (-)	Mated mymarid (+)

Group	Sum of squares	df	Mean square	F	p value
Tea flower	4785.1562	1	4785.1562	42.5105	9.6614 × 10^-10**
Infestation	3001.5563	1	3001.5563	26.6653	7.4251 × 10^-7**
Mating status	1531.4062	1	1531.4062	13.6047	0.0003**
Tea flower × Infestation	693.0563	1	693.0563	6.1570	0.0142*
Tea flower × Mating status	94.5562	1	94.5562	0.8400	0.3608
Infestation × Mating status	45.1562	1	45.1562		0.5274
Sum of squares for error (SSE)	17222.3063	153	112.5641
Total	27373.1938	159

Group	Sum of squares	df	Mean square	F	p value
Tea flower	4785.1562	1	4785.1562	42.5105	9.6614 × 10^-10**
Infestation	3001.5563	1	3001.5563	26.6653	7.4251 × 10^-7**
Mating status	1531.4062	1	1531.4062	13.6047	0.0003**
Tea flower × Infestation	693.0563	1	693.0563	6.1570	0.0142*
Tea flower × Mating status	94.5562	1	94.5562	0.8400	0.3608
Infestation × Mating status	45.1562	1	45.1562		0.5274
Sum of squares for error (SSE)	17222.3063	153	112.5641
Total	27373.1938	159

Group	Mating status	Search time (s)	Spying and spawning time (s)	Total time (s)
Intact tea shoot + tea flower	Virgin	69.75±2.12	10.00±0.30	79.75±2.26
Intact tea shoot + tea flower	Mated	62.50±1.78	9.85±0.28	72.35±1.88
Intact tea shoot	Virgin	74.15±3.02	10.10±0.28	84.25±3.16
Intact tea shoot	Mated	70.90±3.12	10.50±0.34	81.40±3.10
Tea shoot infested by tea green leafhopper + tea flower	Virgin	57.35±1.67	9.90±0.31	67.25±1.70
Tea shoot infested by tea green leafhopper + tea flower	Mated	49.10±2.26	10.10±0.33	59.20±2.15
Tea shoot infested by tea green leafhopper	Virgin	71.50±2.26**	10.05±0.39	81.55±2.36**
Tea shoot infested by tea green leafhopper	Mated	63.95±2.10**	11.15±0.26	75.10±1.99**

茶树花挥发物对叶蝉三棒缨小蜂的引诱增强效应

Enhanced Attraction of Mymarids (Stethynium empoascae) by Volatiles from Tea Flowers

RichHTML

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

图/表 11

参考文献 58

相关文章 15

编辑推荐

Metrics

本文评价

No.	Retention time (min)	Volatile compounds	Relative contents to internal standard (IS)
No.	Retention time (min)	Volatile compounds	Healthy tea shoots	Tea shoots infested by tea green leafhopper	Tea flower
1	3.772	4-penten-2-one	-	4.6679±0.3469	-
2	4.659	2-hexanone	-	-	0.8337±0.1178
3	4.973	3-hexenal	0.6775±0.1432	0.6500±0.0644	-
4	6.791	3-hexen-1-ol	-	2.4906±0.2406	-
5	6.973	Ethylbenzene	-	-	0.4284±0.0374
6	7.045	Z-4-hexen-1-ol	-	3.1843±0.2420	-
7	7.386	m-xylene	0.1821±0.0261	0.9410±0.1252	1.0658±0.1013
8	8.216	p-xylene	0.1388±0.0163	0.1685±0.0114	-
9	8.571	2-heptanone	-	-	15.7670±0.2817
10	9.415	Anisole	-	-	0.4104±0.0497
11	10.061	α-pinene	-	0.0849±0.0098	0.0467±0.0056
12	11.542	Benzaldehyde	-	0.2588±0.0264	0.5192±0.0615
13	12.270	Phenol	-	0.0915±0.0094	-
14	13.383	Decane	-	0.0952±0.0098	0.0424±0.0048
15	13.595	Octanal	-	0.2073±0.0243	0.2724±0.0241
16	13.675	Z-3-hexenyl acetate	-	0.3178±0.0256	-
17	13.694	E-3-hexenyl acetate	-	0.6841±0.0819	-
18	14.841	2-ethyl-1-hexanol	0.1636±0.0303	1.5707±0.1608	1.0125±0.1174
19	15.720	α-phellandrene	-	-	0.1193±0.0104
20	16.061	2-pentylcyclopentanone	-	-	4.9586±0.3190
21	16.373	α-methyl benzyl alcohol	-	-	0.100 0±0.0116
22	16.612	Acetophenone	0.0443±0.0077	1.0436±0.1229	36.3044±3.7347
23	16.900	cis-linaloloxide	-	-	0.0395±0.0043
24	17.713	trans-linaloloxide	-	-	0.1566±0.0081
25	18.460	Undecane	0.0297±0.0048	0.2721±0.0370	0.2192±0.0237
26	18.693	Nonanal	0.0615±0.0105	1.0990±0.1109	0.7224±0.0835
27	20.668	Camphor	0.0989±0.0142	1.2977±0.1150	0.4835±0.0361
28	21.928	E-2-nonen-1-ol	0.0605±0.0074	0.2599+0.0135	0.1866±0.0158
29	22.381	Naphthalene	0.0305±0.0046	0.1241±0.0086	0.2344±0.0224
30	22.662	cis-3-hexenyl butyrate	-	0.0891±0.0081	-
31	23.362	Dodecane	0.0176±0.0029	0.2182±0.0153	0.1281±0.0155
32	23.654	Decanal	0.0995±0.0176	1.1579±0.1294	0.8621±0.0459
33	28.027	Tridecane	-	0.1029±0.0097	0.0625±0.0079
/	32.223	Decanoic acid, ethyl ester (IS)	1.0000	1.0000	1.0000
34	32.423	Tetradecane	0.0261±0.0055	0.3894±0.0406	0.1098±0.0113
35	32.625	Longifolene-(V4)	-	0.1815±0.0268	0.0307±0.0012
36	32.822	Tetradecanal	-	0.0467±0.0030	0.0275±0.0023
37	34.362	E-6,10-dimethyl-5,9-undecadien-2-one	0.0501±0.0065	0.5364±0.0388	0.2798±0.0269
38	36.594	Pentadecane	0.0704±0.0090	0.3610±0.0406	0.0922±0.0051
39	40.532	Hexadecane	0.0552±0.0097	0.3384±0.0304	0.1625±0.0221
40	44.276	Heptadecane	-	0.1006±0.0058	0.0437±0.0053
41	49.699	1,2-benzenedicarboxylic acid, bis(2- methylpropyl) ester	-	-	0.0411±0.0023
42	50.477	Homomenthyl salicylate	-	-	0.0353±0.0041

Classification	Volatile compounds
Green leaf volatiles	3-hexenal, 3-hexen-1-ol, Z-4-hexen-1-ol, Z-3-hexenyl acetate, E-3-hexenyl acetate, 2-ethyl-1-hexanol
Aromatics	Ethylbenzene, m-xylene, p-xylene, anisole, benzaldehyde, phenol, α-methylbenzyl alcohol, acetophenone, naphthalene, 1,2-benzenedicarboxylic acid, bis (2-methylpropyl) ester
Terpenes	4-penten-2-one, α-pinene, α-phellandrene, cis-linaloloxide, trans-linaloloxide, E-2-nonen-1-ol, cis-3- hexenyl butyrate, longifolene-(V4), E-6,10-dimethyl-5,9-undecadien-2-one
Alkanes	Decane, undecane, dodecane, tridecane, tetradecane, pentadecane, hexadecane, heptadecane
Other	2-hexanone, 2-heptanone, octanal, 2-pentylcyclopentanone, nonanal, camphor, decanal, tetradecanal, homomenthyl salicylate

[1]	任露露, 张有泽, 黄克林, 宛晓春, 张照亮, 朱木兰, 韦朝领. 茶树茎段不定芽高效发生体系的建立[J]. 植物学报, 2023, 58(2): 308-315.
[2]	孙廷哲, 岂泽华, 梁可欣, 李沁, 饶玉春, 穆丹. 蚜害茶树挥发物组分变化的聚类分析[J]. 植物学报, 2021, 56(4): 422-432.
[3]	刘建福, 陈育才, 王文建, 王河川, 蔡金福, 王明元, 李丹丹, 张斌, 黄昆. 航天搭载对武夷名丛相关生理及生长特性的影响[J]. 植物学报, 2020, 55(5): 564-572.
[4]	杨小青,黄晓琴,韩晓阳,刘腾飞,岳晓伟,伊冉. 外源物质对茶树耐寒及蔗糖代谢关键基因表达的影响[J]. 植物学报, 2020, 55(1): 21-30.
[5]	张晓玲,李亦超,王芸芸,蔡宏宇,曾辉,王志恒. 未来气候变化对不同国家茶适宜分布区的影响[J]. 生物多样性, 2019, 27(6): 595-606.
[6]	刘小妹,孙丽莉,傅向东,廖红. 茶树嫩枝扦插的高效方法[J]. 植物学报, 2019, 54(4): 531-538.
[7]	李春杰, 姚祥, 南志标. 醉马草内生真菌共生体研究进展[J]. 植物生态学报, 2018, 42(8): 793-805.
[8]	孙燕, 周忠实, 王瑞, HeinzMüller-Schärer. 气候变化预计会减少东亚地区豚草的生物防治效果^**[J]. 生物多样性, 2017, 25(12): 1285-1294.
[9]	林郑和, 钟秋生, 陈常颂, 陈志辉, 游小妹. 不同香型茶树鲜叶挥发性组分与β-葡萄糖苷酶的相关性分析[J]. 植物学报, 2015, 50(6): 713-720.
[10]	李秀璋, 姚祥, 李春杰, 南志标. 禾草内生真菌作为生防因子的潜力分析[J]. 植物生态学报, 2015, 39(6): 621-634.
[11]	黄伟, 王毅, 丁建清. 入侵植物乌桕防御策略的适应性进化研究[J]. 植物生态学报, 2013, 37(9): 889-900.
[12]	强胜, 陈国奇, 李保平, 孟玲. 中国农业生态系统外来种入侵及其管理现状[J]. 生物多样性, 2010, 18(6): 647-659.
[13]	余香琴, 冯玉龙, 李巧明. 外来入侵植物飞机草的研究进展与展望[J]. 植物生态学报, 2010, 34(5): 591-600.
[14]	孙美莲, 王云生, 杨冬青, 韦朝领, 高丽萍, 夏涛, 单育, 骆洋. 茶树实时荧光定量PCR分析中内参基因的选择[J]. 植物学报, 2010, 45(05): 579-587.
[15]	王万能全学军肖崇刚. 烟草内生细菌防治烟草黑胫病及促生作用研究[J]. 植物学报, 2005, 22(04): 426-431.