Chin Bull Bot ›› 2019, Vol. 54 ›› Issue (4): 503-508.doi: 10.11983/CBB19087

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

Protocols for Analyzing Rice Meiotic Chromosomes

Cheng Xinjie1,Yu Hengxiu1,Cheng Zhukuan2,*()   

  1. 1 Agriculture College, Yangzhou University, Yangzhou 225009, China
    2 Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
  • Received:2019-05-12 Accepted:2019-06-14 Online:2019-07-01 Published:2019-07-01
  • Contact: Cheng Zhukuan E-mail:zkcheng@genetics.ac.cn

Abstract:

The development of techniques to analyze pachytene chromosomes has greatly overcome most of the difficulties in cytological studies of rice chromosomes caused by their small size. Visualization of meiotic chromosomes has now become routine in cytogenetic studies of this species. This chapter provides protocols on basic meiotic chromosome preparation, FISH analysis and immunocytology in rice.

Key words: rice, meiosis, chromosome, FISH, immunostaining

Figure 1

Characterization of rice meiotic pachytene chromosomes (A) Fluorescent in situ hybridization using rice pachytene chromosomes probed with the telomeric sequence pAtT4 (red); Chromosomes stained with DAPI (blue) (Images taken with a regular fluorescence microscope); (B) Immunodetection of PAIR3 (green) and ZEP1 (red) in rice pachytene chromosomes (Images taken with a super resolution microscope). Bars=5 µm"

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