Helenium aromaticum has a fragrant odor and its capitulum contains only disk flowers. H. aromaticum is a good model for studying development of floral patterns in Compositae. However, methods for genetic transformation of H. aromaticum is currently not available. In this study, we report the establishment of an efficient in vitro regeneration system of the H. aromaticum. The induction of adventitious buds was tested with various combinations and concentrations of 25 different phytohormones by using leaves, stem segments, and hypocotyls as explants. We found that when leaf explants were cultured on MS medium supplemented with 0.2 mg·L-1 NAA, 1 mg·L-1 6-BA, and 0.2 mg·L-1 TDZ for 20 days, the induction of callus was 100% and the induction rate of adventitious buds was 62.10%. The newly-formed adventitious buds were then cultured on 1/2MS medium for 16 days and the rooting rate reached 63.33%. After culturing for an additional 14 days, floral buds initiated and eventually flowering with a rate of 93.33%. In addition, we also found that the regeneration of H. aromaticum was affected by the nature of explants, the types and concentrations of phytohormones. Whereas 2,4-D is incapable of inducing adventitious buds, the combination of appropriate concentrations of 6-BA and TDZ effectively promotes the formation of adventitious buds of H. aromaticum. This study has established an in vitro regeneration system of H. aromaticum, which is a key perquisite for the subsequent establishment of its genetic transformation system. Moreover, this method will also be an important reference for studies on the floral development patterns in Compositae.