Chinese Bulletin of Botany ›› 2019, Vol. 54 ›› Issue (4): 509-514.DOI: 10.11983/CBB19101

Special Issue: 逆境生物学专辑

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

Identification of Chilling Tolerance of Rice Seedlings by Cold Water Bath

Dongfeng Liu,Yongyan Tang,Shengtao Luo,Wei Luo,Zhitao Li,Kang Chong,Yunyuan Xu()   

  1. Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
  • Received:2019-05-29 Accepted:2019-06-11 Online:2019-07-10 Published:2020-01-08
  • Contact: Yunyuan Xu


The premise of studies on rice chilling tolerance is to find an efficient and accurate way to evaluate chilling tolerance of rice seedling. In this study, we developed an efficient technology to evaluate the cold tolerance at seedling stage by using a constant temperature water bath, based on the characteristics of excellent temperature uniformity for circulation of water. In this method, the setting temperature of environment temperature and water bath was 20°C and 4°C, respectively. From the results of two subspecies (indica/xian and japonica/geng), we summarized the reference treatment time for different survival rate of cultivars. Some attention to the cold treatment procedure was also discussed.

Key words: rice, seedling, cold tolerance, low temperature water bath