Chinese Bulletin of Botany ›› 2019, Vol. 54 ›› Issue (4): 503-508.DOI: 10.11983/CBB19087 cstr: 32102.14.CBB19087
• TECHNIQUES AND METHODS • Previous Articles Next Articles
Xinjie Cheng1,Hengxiu Yu1,Zhukuan Cheng2,*(
)
Received:2019-05-12
Accepted:2019-06-14
Online:2019-07-01
Published:2019-07-01
Contact:
Zhukuan Cheng
Xinjie Cheng, Hengxiu Yu, Zhukuan Cheng. Protocols for Analyzing Rice Meiotic Chromosomes[J]. Chinese Bulletin of Botany, 2019, 54(4): 503-508.
Figure 1 Characterization of rice meiotic pachytene chromosomes (A) Fluorescent in situ hybridization using rice pachytene chromosomes probed with the telomeric sequence pAtT4 (red); Chromosomes stained with DAPI (blue) (Images taken with a regular fluorescence microscope); (B) Immunodetection of PAIR3 (green) and ZEP1 (red) in rice pachytene chromosomes (Images taken with a super resolution microscope). Bars=5 µm
| [1] | Arumuganathan K, Earle ED ( 1991). Estimation of nuclear DNA content of plants by flow cytometry. Plant Mol Biol Rep 9, 229-241. |
| [2] | Che L, Tang D, Wang K, Wang M, Zhu K, Yu H, Gu M, Cheng Z ( 2011). OsAM1 is required for leptotene transition in rice. Cell Res 21, 654-665. |
| [3] | Cheng Z, Buell CR, Wing RA, Gu M, Jiang J ( 2001 a). Toward a cytological characterization of the rice genome. Genome Res 11, 2133-2141. |
| [4] | Cheng Z, Dong F, Langdon T, Ouyang S, Buell CR, Gu M, Blattner FR, Jiang J ( 2002). Functional rice centromeres are marked by a satellite repeat and a centromere-specific retrotransposon. Plant Cell 14, 1691-1704. |
| [5] | Cheng Z, Stupar RM, Gu M, Jiang J ( 2001 b). A tandemly repeated DNA sequence is associated with both knob-like heterochromatin and a highly decondensed structure in the meiotic pachytene chromosomes of rice. Chromosoma 110, 24-31. |
| [6] | Goff SA ( 1999). Rice as a model for cereal genomics. Curr Opin Plant Biol 2, 86-89. |
| [7] | Ji JH, Tang D, Shen Y, Xue ZH, Wang HJ, Shi WQ, Zhang C, Du GJ, Li YF, Cheng ZK ( 2016). P31 comet, a member of the synaptonemal complex, participates in meiotic DSB formation in rice . Proc Natl Acad Sci USA 113, 10577-10582. |
| [8] | Kurata N, Omura T, Iwata N ( 1981). Studies on centromere, chromomere and nucleolus in pachytene nuclei of rice, Oryza sativa, microsporocytes. Cytologia 46, 791-800. |
| [9] | Li X, Chao D, Wu Y, Huang X, Chen K, Cui L, Su L, Ye W, Chen H, Chen H, Dong N, Guo T, Shi M, Feng Q, Zhang P, Han B, Shan J, Gao J, Lin H ( 2015). Natural alleles of a proteasome α2 subunit gene contribute to thermotolerance and adaptation of African rice. Nat Genet 47, 827-833. |
| [10] | Li YF, Qin BX, Shen Y, Zhang FF, Liu CZ, You HL, Du GJ, Tang D, Cheng ZK ( 2018). HEIP1 regulates crossover formation during meiosis in rice. Proc Natl Acad Sci USA 115, 10810-10815. |
| [11] | Luo Q, Li YF, Shen Y, Cheng ZK ( 2014). Ten years of gene discovery for meiotic event control in rice. J Genet Genomics 41, 125-137. |
| [12] | Ma Y, Dai X, Xu Y, Luo W, Zheng X, Zeng D, Pan Y, Lin X, Liu H, Zhang D, Xiao J, Guo X, Xu S, Niu Y, Jin J, Zhang H, Xu X, Li L, Wang W, Qian Q, Ge S, Chong K ( 2015). COLD1 confers chilling tolerance in rice. Cell 160, 1209-1221. |
| [13] | Miao CB, Tang D, Zhang HG, Wang M, Li YF, Tang SZ, Yu HX, Gu MH, Cheng ZK ( 2013). CENTRAL REGION COMPONENT 1, a novel synaptonemal complex component, is essential for meiotic recombination initiation in rice. Plant Cell 25, 2998-3009. |
| [14] | Nonomura K, Morohoshi A, Nakano M, Eiguchi M, Miyao A, Hirochika H, Kurata N ( 2007). A germ cell specific gene of the ARGONAUTE family is essential for the progression of premeiotic mitosis and meiosis during sporogenesis in rice. Plant Cell 19, 2583-2594. |
| [15] | Pawlowski WP, Golubovskaya IN, Timofejeva L, Meeley RB, Sheridan WF, Cande WZ ( 2004). Coordination of meiotic recombination, pairing, and synapsis by PHS1. Science 303, 89-92. |
| [16] | Ren LJ, Tang D, Zhao TT, Zhang FF, Liu CZ, Xue ZH, Shi WQ, Du GJ, Shen Y, Li YF, Cheng ZK ( 2018). OsSPL regulates meiotic fate acquisition in rice. New Phytol 218, 789-803. |
| [17] | Tang X, Bao W, Zhang W, Cheng Z ( 2007). Identification of chromosomes from multiple rice genomes using a universal molecular cytogenetic marker system. J Integr Plant Biol 49, 953-960. |
| [18] | Wang K, Tang D, Wang M, Lu J, Yu H, Liu J, Qian B, Gong Z, Wang X, Chen J, Gu M, Cheng Z ( 2009). MER3 is required for normal meiotic crossover formation, but not for presynaptic alignment in rice. J Cell Sci 122, 2055-2063. |
| [19] | Wang M, Wang K, Tang D, Wei C, Li M, Shen Y, Chi Z, Gu M, Cheng Z ( 2010). The central element protein ZEP1 of the synaptonemal complex regulates the number of crossovers during meiosis in rice. Plant Cell 22, 417-430. |
| [20] | Wang Y, Xiong G, Hu J, Jiang L, Yu H, Xu J, Fang Y, Zeng L, Xu E, Xu J, Ye W, Meng X, Liu R, Chen H, Jing Y, Wang Y, Zhu X, Li J, Qian Q ( 2015). Copy number variation at the GL7 locus contributes to grain size diversity in rice. Nat Genet 47, 944-948. |
| [21] | Wu HK ( 1967). Note on preparing of pachytene chromosomes by double mordant. Sci Agric 15, 40-44. |
| [22] | Yu H, Wang M, Tang D, Wang K, Chen F, Gong Z, Gu M, Cheng Z ( 2010). OsSPO11-1 is essential for both homologous chromosome pairing and crossover formation in rice. Chromosoma 119, 625-636. |
| [23] | Zhang F, Tang D, Shen Y, Xue ZH, Shi WQ, Ren LJ, Du GJ, Li Y, Cheng ZK ( 2017). The F-box protein ZYGO1 mediates bouquet formation to promote homologous pairing, synapsis, and recombination in rice meiosis. Plant Cell 29, 2597-2609. |
| [24] | Zhao TT, Ren LJ, Chen XJ, Yu HX, Liu CJ, Shen Y, Shi WQ, Tang D, Du GJ, Li YF, Ma BJ, Cheng ZK ( 2018). The OsRR24/LEPTO1 type-B response regulator is essential for the organization of leptotene chromosomes in rice meiosis. Plant Cell 30, 3024-3037. |
| [1] | Tianqi He, Dongdong Zhai, Fangtao Cai, Dong Wang, Yuanyuan Chen, Hongyan Liu, Fei Xiong, Mingdian Liu, Xinbin Duan. Characteristics of fish community structure in the lower mainstream of the Lancang River and risk assessment of alien fish invasion [J]. Biodiv Sci, 2026, 34(5): 25394-. |
| [2] | Jinhua Yang, Bing Wang. Precision Engineering of Strigolactone Receptor Aids Rice in Antiviral Defense [J]. Chinese Bulletin of Botany, 2026, 61(3): 357-368. |
| [3] | Xuri Zhang, Biao Luo, Tong Zhao, Dan Huang, Weiming Ai. Fish diversity of Zhejiang Province: Inventory, distribution and conservation [J]. Biodiv Sci, 2026, 34(2): 25225-. |
| [4] | FAN Yue-Ling, JIANG Zheng-De, YE Jia-Shu, ZHENG Li-Chen, CHEN Xin. Dataset on harvest traits and yield of major crops in long-term farmland observation plots in the Lower Liaohe Plain from 2005 to 2015 [J]. Chin J Plant Ecol, 2025, 49(8): 1271-1282. |
| [5] | Zhongwen Ding, Yiting Chen, Wen Yu, Jingjing Zhang, Yibin Huang, Dingke Li, Zhaojie Peng, Han Lai, Shichao Wei, Mingpan Huang. Application of baited remote underwater video technology in assessing nearshore reef fish diversity [J]. Biodiv Sci, 2025, 33(6): 24572-. |
| [6] | Zhengdong Pan, Xirong Lin, Hua Xue, Zhiying Hu, Hongyi Guo, Ya Zhang, Enuo Wu, Wenqiao Tang. Fish species diversity background and community structure in the main inland water bodies of Shanghai [J]. Biodiv Sci, 2025, 33(6): 24290-. |
| [7] | Jiang Yanan, Xu Yuqing, Wei Yiting, Chen Jun, Zhang Rongwan, Zhao Beibei, Lin Yuxiang, Rao Yuchun. Research Progress on the Regulatory Mechanism of Rice Disease Resistance [J]. Chinese Bulletin of Botany, 2025, 60(5): 734-748. |
| [8] | Juan Cui, Xiaoyu Yu, Yuejiao Yu, Chengwei Liang, Jian Sun, Wenfu Chen. Analysis of the Texture Factors and Genetic Basis Influencing the Differences in Eating Quality between Northeast China and Japanese Japonica Rice [J]. Chinese Bulletin of Botany, 2025, 60(4): 533-550. |
| [9] | Can Ye, Linbo Yao, Ying Jin, Rong Gao, Qi Tan, Xuying Li, Yanjun Zhang, Xifeng Chen, Bojun Ma, Wei Zhang, Kewei Zhang. Establishment and Application of a High-throughput Screening Method for Salicylic Acid Metabolic Mutants in Rice [J]. Chinese Bulletin of Botany, 2025, 60(4): 586-596. |
| [10] | Wang Tai, Song Fujun, Zhang Yongsheng, Lou Zhongyu, Zhang Yanping, Du Yanyan. Fish diversity and resource status in interior drainage systems of Hexi Corridor [J]. Biodiv Sci, 2025, 33(4): 24387-. |
| [11] | Zhao Ling, Guan Ju, Liang Wenhua, Zhang Yong, Lu Kai, Zhao Chunfang, Li Yusheng, Zhang Yadong. Mapping of QTLs for Heat Tolerance at the Seedling Stage in Rice Based on a High-density Bin Map [J]. Chinese Bulletin of Botany, 2025, 60(3): 342-353. |
| [12] | Xinyu Li, Yue Gu, Feifei Xu, Jinsong Bao. Research Progress on Post-translational Modifications of Starch Biosynthesis-related Proteins in Rice Endosperm [J]. Chinese Bulletin of Botany, 2025, 60(2): 256-270. |
| [13] | Ma Wenjun, Liu Sijia, Li Kemao, Jian Shenglong, Xue Chang’an, Han Qingxiango, Wei Jinliang, Chen Shengxue, Niu Yimeng, Cui Zhouping, Sui Ruichen, Tian Fei, Zhao Kai. Fish diversity and distribution in the source region of the Yangtze River in Qinghai Province [J]. Biodiv Sci, 2025, 33(2): 24494-. |
| [14] | Xiao Chen, Xiaohui Ma, Jiacheng Chen, Ding Yao, Xinyi Yu, Wenfei Song, Zhongguan Jiang, Shiping Su. Updated species checklist of freshwater fishes from major tributaries of Yangtze River in Chizhou of Anhui Province, China [J]. Biodiv Sci, 2025, 33(12): 25182-. |
| [15] | Jianguo Li, Yi Zhang, Wenjun Zhang. Iron Plaque Formation and Its Effects on Phosphorus Absorption in Rice Roots [J]. Chinese Bulletin of Botany, 2025, 60(1): 132-143. |
| Viewed | ||||||
|
Full text |
|
|||||
|
Abstract |
|
|||||