Chin Bull Bot ›› 2019, Vol. 54 ›› Issue (3): 296-299.doi: 10.11983/CBB19033

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Chinese Scientists Reveal Genome-wide Off-targeted Editing of Cytosine Base Editor

Xie Kabin()   

  1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China
  • Received:2019-01-21 Accepted:2019-01-22 Online:2019-05-20 Published:2019-05-01
  • Contact: Xie Kabin E-mail:kabinxie@mail.hzau.edu.cn

Abstract:

Targeted base editing with CRISPR-Cas systems is a breakthrough in genome editing technologies and is widely used in studies of humans, animals and plants. Recently, Chinese scientists discovered that the cytosine base editor (CBE) including BE3 and HF1-BE3 but not the adenine base editor (ABE) has substantial promise for genome-wide off-targeted editing in rice. This discovery is of great significance to the application and further optimization of targeted base editing.

Key words: base editing, CRISPR, off-targeting

Figure 1

CRISRP-Cas9 base editing(A) Deaminase-mediated base editing reactions; (B) The schematic structure of cytosine (CBE, take BE3 as an example) and adenine (ABE) base editors. L: Peptide linker"

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