Chinese Bulletin of Botany ›› 2019, Vol. 54 ›› Issue (2): 227-236.doi: 10.11983/CBB18142

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The Effect of Molybdenum on Drought Stress Response in Peach

Gao Huaifeng,Zhang Yafei,Wang Guodong,Sun Xiwu,He Yue,Peng Futian(),Xiao Yuansong()   

  1. State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, China
  • Received:2018-06-19 Accepted:2018-11-16 Online:2019-09-01 Published:2019-03-01
  • Contact: Peng Futian,Xiao Yuansong E-mail:pft@sdau.edu.cn;ysxiao@sdau.edu.cn

Abstract:

Peach (Amygdalus persica) seedlings were used to analyze the effect of ammonium molybdate on the expression of molybdenum cofactor sulfurase gene (LOS5/ABA3), ABA content and physiological indexes related to drought resistance under drought stress. Water, chlorophyll and proline contents of peach seedling leaves were significantly higher with ammonium molybdate, preferably 0.04%, than control treatment, and electrolyte leakage was lower. Under drought stress, the expression of LOS5/ABA3 in leaves treated with 0.04% ammonium molybdate was significantly increased and ABA content, water use efficiency, and net photosynthetic rate was higher than with control treatment. Transpiration rate was lower, antioxidant enzyme activity was higher, and malondialdehyde content was lower in leaves; the quality loss of leaves in vitro was reduced. Thus, ammonium molybdate treatment of peach seedlings can increase the content of ABA and proline, increase the activity of antioxidant enzymes, relieve cell membrane oxidation damage, decrease the rate of water loss of leaves, and alleviate drought stress damage by regulating the expression of drought- resistant genes, then increase the drought resistance of plants.

Key words: peach seedlings, ammonium molybdate, drought stress, LOS5/ABA3, drought resistance

Table 1

Primers used in this study"

Primer name Primer (5′→3′)
PpABA3-F CAATGGTACTGCAGCATCTATGA
PpABA3-R TAAGTTATATGCCTCTCCTGTTGG
PpACTIN-F TGCATTGTGTATGTGTTCATCTACA
PpACTIN-R CTTCACCATTCCAGTTCCATTGTC

Figure 1

Water content (A) and electrolyte leakage (B) of peach seedlings treated with different concentrations of ammonium molybdate under drought stress Different lowercase letters above the bars indicate significant differences at 0.05 level."

Figure 2

Chlorophyll (A) and proline (B) content of peach seedlings treated with different concentrations of ammonium molybdate under drought stressDifferent lowercase letters above the bars indicate significant differences at 0.05 level."

Figure 3

The SPAD (A), net photosynthetic rate (B), transportation rate (C) and water use efficiency (D) of peach seedlings treated with ammonium molybdate under drought stress Different lowercase letters above the bars indicate significant differences at 0.05 level."

Figure 4

The changes of the quality percentage of detached leaves of peach CK: Normal conditions+water; T1: Normal conditions+0.04% ammonium molybdate; T2: Medium drought stress+water; T3: Medium drought stress+0.04% ammonium molybdate; T4: Severe drought stress+water; T5: Severe drought stress+ 0.04% ammonium molybdate. Different lowercase letters above the bars indicate significant differences at 0.05 level."

Figure 5

The effect of ammonium molybdate on the MDA content (A) and the activity of CAT (B), POD (C) and SOD (D) of peach seedlingsMDA: Malondialdehyde; CAT: Catalase; POD: Peroxidase; SOD: Superoxide dismutase. Different lowercase letters above the bars indicate significant differences at 0.05 level."

Figure 6

ABA content of peach seedlings treated with ammonium molybdate under drought stress Different lowercase letters above the bars indicate significant differences at 0.05 level."

Figure 7

The expression of LOS5/ABA3 in leaves of peach seedlings under different drought stresses(A) Normal conditions; (B) Medium drought stress; (C) Severe drought stress. Different lowercase letters above the bars indicate significant differences at 0.05 level."

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