Chinese Bulletin of Botany ›› 2019, Vol. 54 ›› Issue (2): 237-244.DOI: 10.11983/CBB18093

Special Issue: 逆境生物学专辑

• TECHNIQUE AND METHOD • Previous Articles     Next Articles

Analysis of Temperature and Light Factors during Frost Events and Establishing Conditions for Simulated Frost

Lulong Sun1,2,Qiuyan Duan3,Heng Zhai1,*(),Yuanpeng Du1,*()   

  1. 1 State Key Laboratory of Crop Biology, College of Horticultural Science and Engineering, Shandong Agricultural University, Tai’an 271018, China
    2 College of Agriculture, Guizhou University, Guiyang 550025, China
    3 Dezhou Municipal Gardening Administration Bureau, Dezhou 253000, China
  • Received:2018-04-10 Accepted:2018-06-20 Online:2019-03-10 Published:2019-09-01
  • Contact: Heng Zhai,Yuanpeng Du


Frost has had a prominent influence on the fruit industry in China in recent years. We need to establish a system to simulate frost treatment for fruit trees. Based on observations of frost events in field, we analyzed the characteristics of frost in terms of cooling rate, the low temperature limit, warming rate, and light conditions after frost treatment and established a system to simulate frost treatment in the laboratory. Temperature during the frost treatment in field could be divided into three stages: cooling, extreme temperature maintenance and warming. The temperature during cooling and warming stages changed in an approximately linear manner. Frost is generally followed by high intensity light. The simulated frost process was determined as followed: the temperature drops from room temperature (20°C) to 5°C in 30 min, and is maintained at 5°C for 30 min, then decreases to -2°C at a rate of 0.8°C·h -1, is maintained at -2°C for 2 h, then increases to 5°C at a rate of 4.7°C·h -1 in the dark. The recovery condition after frost was 16°C and 800 μmol·m -2·s -1.

Key words: frost, temperature, light, simulation