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  • Hosted by:Chinese Academy of Sciences
    Sponsored by:Institute of Botany, Chinese Academy of Sciences, Botanical Society of China
    Co-hosted by:Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences
    Institute of Biotechnology and Germplasm Resources, Yunnan AgriculturalAcademy
    Fujian Agriculture and Forestry University
    Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University
    State Key Laboratory of Crops Biology, Shandong Agricultural University

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Identification of TCP Gene Family and Functional Analysis of TCP15a of Tree Peony
Chinese Bulletin of Botany    DOI: 10.11983/CBB25005
Accepted: 18 March 2025

Abstract119)      PDF (2824KB)(98)       Save
  
Differences in Adaptive Development of Suberin in Tobacco Root Endothelial Layer under Different Potassium Levels
Yunxiang Xu, Liwen Zhang, Peng Wang, Yingchen Gu, Madan Lal Kolhi, Biao Zhang, Yingying Zhu, Haiwei Liu
Chinese Bulletin of Botany    DOI: 10.11983/CBB24191
Accepted: 18 March 2025

Abstract68)      PDF (2887KB)(33)       Save
  
Cloning and Functional Analysis of 14-3-3 Protein gene TaGRF3-D in wheat (Triticum aestivum)
Yue Sun, Shujuan Guo, Huixian Zhao, Meng Ma, Xiangli Liu
Chinese Bulletin of Botany    DOI: 10.11983/CBB24156
Accepted: 18 March 2025

Abstract76)      PDF (2046KB)(61)       Save
  
Preliminary Analysis of Function of the Gene GhDIR1 Related With Verticillium Wilt in Cotton
Yuxin Huang, Xie Tao, Xingfen Wang, Huiming Guo, Hongmei Cheng, Bojun Ma, Xifeng Chen, Xiaofeng Su
Chinese Bulletin of Botany    DOI: 10.11983/CBB24135
Accepted: 15 November 2024

Abstract123)      PDF (1873KB)(118)       Save
  
Cloning and Functional Analysis of APETALA3/DEFICIENS Homologous Gene from Rhus chinensis
Lei Gu, Qi Zhang, Xia Zhang, Bingbing Yang, Fanglan Wang, Wen Liu, Faju Chen
Chinese Bulletin of Botany    2024, 59 (4): 533-543.   DOI: 10.11983/CBB24015
Accepted: 17 April 2024

Abstract353)   HTML40)    PDF (11681KB)(358)    PDF(mobile) (11681KB)(23)    Save

The APETALA3/DEFICIENS (AP3/DEF) gene is a B-class gene of the MADS-box gene family, which is mainly involved in the regulation of petal and stamen development during flower development. Cloning and analysis of the AP3/DEF homologous genes from Rhus chinensis can help exploring their role in the development of stamen. The CDS of AP3/DEF homologous gene was obtained using RT-PCR; its sequence and structural domains were compared and analyzed by NCBI CD Search; interactions between AP3/DEF homologous proteins and other MADS-box transcription factors in R. chinensis were verified by using yeast two-hybrid system; real-time fluorescence PCR was used to analyze the spatial and temporal expression patterns of the AP3/DEF homologous genes; overexpression in Arabidopsis was conducted to verify the function of the AP3/DEF homologous genes in flower organ development. Two coding sequences of AP3/ DEF homologous genes in R. chinensis were cloned and named RcAP3 (GenBank: OR962160) and RcTM6 (GenBank: OR962159). Based on the alignment of their conserved amino acid domains and phylogenetic analysis, these two protein sequences showed the closest genetic relationship to the AP3/DEF homologous gene-encoded proteins in the Anacardiaceae family, including Mangifera indica and Pistacia vera. The yeast two-hybrid results showed that RcAP3 and RcTM6 interacted with the B-class protein RcPI, the C-class proteins RcAG and Rcag in R. chinensis, but not with the A-class and E-class proteins. Real-time fluorescence quantification results demonstrated that RcAP3 and RcTM6 were highly expressed during the rapid growth stage of flower buds in different sexes of R. chinensis, with lower expression levels during the early bud development and after flowering. RcAP3 exhibited high expression levels during buds differentiation in female, male, and hermaphrodite flowers, while RcTM6 showed significant expression in hermaphrodite flowers and very low expression in male and female flowers. During the rapid growth stage of hermaphrodite flowers, RcAP3 was highly expressed in both petals and stamens with minimal differences, whereas RcTM6 showed significantly higher expression in stamens compared to other floral organs. RcAP3 could restore the petal and stamen phenotypes in the ap3-3 mutant of Arabidopsis, while overexpression of RcTM6 resulted in shortened petals, stamens, and aborted ovaries in Arabidopsis. Functional differentiation exists between the AP3/DEF subfamily homologous genes, RcAP3 and RcTM6. RcAP3 promotes petal and stamen development, while RcTM6 inhibits stamen development. These findings provide a foundation for further research on the molecular mechanisms underlying sex differentiation in R. chinensis.

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