Preliminary Analysis of Function of the Gene GhDIR1 Related With Verticillium Wilt in Cotton

doi:10.11983/CBB24135

Abstract

Abstract: Verticillium wilt, caused by Verticillium dahliae, is one of the most major threats in cotton production, which can lead to a significant reduction of cotton yield and fiber quality. Our research team previously conducted a transcriptomic analysis on Arabidopsis thaliana inoculated with V. dahliae and discovered that the gene AT3G53980.2, which encodes the DIR1-like protein, was strongly induced by the pathogen. In this study, we discovered that the cotton lipid transfer protein gene GhDIR1 (Gh_A09G180700.1) displays high homology with AT3G53980.2. Bioinformatic analysis indicated that the open reading frame (ORF) of GhDIR1 is 351 bp, encoding 116 amino acids. The subcellular localization analysis using transient expression displayed that GhDIR1 was shown to be localized to the cell membrane. Quantitative real-time PCR (qRT-PCR) analysis determined that GhDIR1 expression was rapidly induced by V. dahliae. Virus-induced gene silencing revealed that downregulation of GhDIR1 significantly increased cotton susceptibility to V. dahliae. Further analysis through transcriptomic sequencing of wild-type and GhDIR1-silenced plants revealed that differentially expressed genes were primarily enriched in pathways related to "flavonoid biosynthesis","sesquiterpene and triterpene biosynthesis" and "α-linolenic acid metabolism". The expression levels of marker genes (GhCHS, GhDFR, GhCAD, GhSEQ, GhLOX, and GhAOC) involved in three pathways were downregulated. This data suggests that GhDIR1 contributes to the synthesis of protective compounds. We propose that GhDIR1 may mediate the synthesis of flavonoids and terpenoids, regulate the secondary metabolism of plant hormones such as jasmonic acid (JA), and activate related signaling pathways. These results suggested that GhDIR1 might act as a positive regulatory factor and modulate immune defense responses through its regulation in multiple hormone and resistance signaling networks against Verticillium wilt in cotton.

Key words: Verticillium wilt, Gossypium hirsutum, GhDIR1, gene expression pattern analysis, virus-induced gene silencing (VIGS)

Yuxin Huang, Xie Tao, Xingfen Wang, Huiming Guo, Hongmei Cheng, Bojun Ma, Xifeng Chen, Xiaofeng Su. Preliminary Analysis of Function of the Gene GhDIR1 Related With Verticillium Wilt in Cotton[J]. Chinese Bulletin of Botany, DOI: 10.11983/CBB24135.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB24135

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