Identification of TCP Gene Family and Functional Analysis of TCP15a of Tree Peony

doi:10.11983/CBB25005

Abstract

Abstract: INTRODUCTION: Flower color is one of the important traits to evaluate the ornamental value of tree peony (Paeonia suffruticosa). Anthocyanin plays a major role in the color of tree peony petals. The biosynthesis of anthocyanin is mainly controlled by two types of genes which are directly involved in pigment metabolism and transcription factors which play a regulatory role. TCP transcription factors are involved in the regulation of secondary metabolism, flavonoid and chlorophyll synthesis in plants. Therefore, it is of great value for further analysis of anthocyanin biosynthesis and regulation mechanism to explore the gene function of TCP family in tree peony and clarify the regulation mechanism of TCP in tree peony petal coloration.

RATIONALE: In order to study the role of TCP transcription factor family in the process of tree peony petal coloration and provide candidate genes for tree peony flower color improvement and molecular breeding, the TCP family of tree peony was identified and analyzed by bioinformatics, and the function of genes TCP13 and TCP15a that may be involved in flower color regulation was analyzed.

RESULTS: Twenty-six TCP gene family members were identified from three tree peony genomes and divided into two subtribes and three subclasses, which were irregularly distributed on 5 chromosomes. The physicochemical properties and gene structure of different members were different, but all members contained TCP conserved domains. The transient expression of TCP13 and TCP15a, which may be involved in petal coloration, showed that TCP13 had no obvious effect on the purple phenotype induced by MYB57, while TCP15a could significantly inhibit it.Fluorescence quantitative PCR analysis showed that TCP15a could significantly inhibit the expression of anthocyanin synthase genes CHSa, DFR and ANS in the tissue of disseminated petals. The interaction between TCP15a and MBW protein complex members WD40-1 and WD40-2 was identified by protein interaction identification.

CONCLUSION: In this study, twenty-six TCP gene family members were screened and identified by mining the tree peony genome database, and the characteristics of TCP encoded amino acids, evolutionary relationship and gene structure were analyzed by bioinformatics.It was found that TCP15a may inhibit the biosynthesis of anthocyanins by competing with MYB57 and interacting with WD40, regulating the expression of enzyme genes CHSa, DFR and ANS, etc. The results provided a theoretical basis for exploring the molecular mechanism of tree peony petal coloration.

Identification of TCP gene family and functional analysis of TCP13 and TCP15a of tree peony. In this study, twenty-six TCP gene family members were screened and identified, and the characteristics of TCP encoded amino acids, evolutionary relationship and gene structure were analyzed by bioinformatics. And.TCP15a was found to inhibit anthocyanin biosynthesis by competing with MYB57 and interacting with WD40.

Key words: Tree peony, flower color, TCP transcription factor family, functional analysis

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB25005

References

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