Chinese Bulletin of Botany ›› 2022, Vol. 57 ›› Issue (5): 649-660.DOI: 10.11983/CBB22049

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

The Chemical Composition and Transcriptome Analysis Reveal the Mechanism of Color Formation in Rosa hybrida cv. ‘Double delight’

Zhang Hechen, Wang Huijuan, Li Yanmin, Gao Jie, Yuan Xin, Wang Limin, Wang Xiaochen, Zhao Yinge, Fu Zhenzhu*()   

  1. Institute of Horticulture Research, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
  • Received:2022-03-12 Accepted:2022-06-23 Online:2022-09-01 Published:2022-09-09
  • Contact: Fu Zhenzhu
  • About author:*E-mail:


Rosa species is one of world-famous ornamental flower with very rich colors. Rosa hybrida cv. ‘Double delight’ is very popular in the market due to the color-changing properties of petals. With analysis of the composition of flavonoids and carotenoids, combined with transcriptome analysis, we showed that the red petals were caused by the increasing of glycosylated anthocyanins, and the up-regulation of anthocyanin synthesis genes CHI, ANS and UFGT by R2R3-MYB transcription factor AN2-likes. However, the yellow petals were mainly due to the accumulation of the pigments of chlorophyll, carotenoids and flavonoids. The structural differences of carotenoids and the expression changes of related genes may play an important role in the petals’ discoloration, and miRNA156 might be involved in the regulation. This study revealed a molecular and chemical basis of the petal discoloration in rose Double delight and provided an important basis for the molecule design and breeding of flowers.

Key words: rose, flower color, anthocyanins, carotenoids, molecular regulation