Figure 1 The phenotype of the petal coloration (A), the difference of flavonoids (B), and the main different components (C) in petals of Rosa hybrida cv. ‘Double delight’ S1: The development stage when sepals are initially unfolded; S2: The development stage when petals are being colored; S3: The development stage when petals are just opened; S4: The development stage when petals are in full bloom

Figure 2 Analysis of differential LncRNA target genes (A) and siRNA target genes (B) clustering in different colored petals of Rosa hybrida cv. ‘Double delight’

Figure 3 Characteristics of gene expression (A) and KEGG clustering of DEGs (B) in different colored petals of Rosa hybrida cv. ‘Double delight’ FDR: False discovery rate

Figure 4 Expression pattern analysis of anthocyanin and carotenoid synthesis-related genes in the petals of Rosa hybrida cv. ‘Double delight’ The blue to red color represents gene expression from weak to strong (the color version is shown in online). *represent genes with significant difference.

Figure 5 Phylogenic analysis of differentially expressed R2R3-MYB and flavonoid regulation related genes (A), and heat map of the corresponding R2R3-MYB members in different colored petals of Rosa hybrida cv. ‘Double delight’ (B) Y and R see Table 2. * represent R2R3-MYB genes related to the regulation of anthocyanin biosynthesis.

Figure 6 qRT-PCR analysis of the expression pattern of the corresponding genes in the Rosa hybrida cv. ‘Double delight’ petals at different developmental stages S1-S4 see Figure 1. * mean significant differences compared with S1 stage.