Chinese Bulletin of Botany ›› 2022, Vol. 57 ›› Issue (4): 508-531.DOI: 10.11983/CBB22020

• SPECIAL TOPICS • Previous Articles     Next Articles

Advance in Gene-editing Technology Based on CRISPR/Cas9 and Its Application in Plants

He Xiaoling, Liu Pengcheng, Ma Bojun, Chen Xifeng()   

  1. College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China
  • Received:2022-01-21 Revised:2022-04-24 Online:2022-07-01 Published:2022-07-14
  • Contact: Chen Xifeng
  • About author:* E-mail:
    First author contact: These authors contributed equally to this paper

Abstract: CRISPR/Cas9 technology is a gene editing strategy using Cas9 nuclease guided by RNA to target an interest gene in genome. Recently, a large number of new-type gene editors based on the CRISPR/Cas9 have been updated rapidly and become more precisely and efficiently in gene editing, which has a great application prospect in crop molecular-designing breeding. This paper summarized the technical principles, editing effects and applications of the CRISPR/cas9 and its related gene editors, and also discussed the aspects of dilemmas, countermeasures and prospects, intending to provide reference for scientific researchers in the related fields.

Key words: CRISPR/Cas9, gene editing, technical progress, application in plants