Heterologous Overexpression of Desiccation-tolerance Moss ScABI3 Gene Changes Stomatal Phenotype and Improves Drought Resistance in Transgenic Arabidopsis

doi:10.11983/CBB20212

Abstract

Abstract: ABI3 is a key transcriptional factor of the ABA signaling pathway, which is involved in seed dormancy, plastid development, and desiccation tolerance of bryophytes, and plays an important role in the stress tolerance of plant. This study obtained three independent pCAMBIA1301-ScABI3 transgenic Arabidopsis homozygous lines. The results showed that transgenic plants increased leaf stomata diameter, reduced the number of stomata in unit area, and improved plant water use efficiency; transgenic plants survival rate was significantly higher than wild type (WT) after 14 days of drought treatment; the water loss rate of transgenic leaves was significantly lower than that of WT. Further research has found that ScABI3 transgenic plants had higher drought resistance characteristics by improving reactive oxygen species (ROS) scavenging ability. These results may be contributed to the development and utilization of genetic resources of desert plants, also provide the theoretical and practical base for molecular breeding.

Key words: abscisic acid, ABI3, drought resistance, stomata

Yigong Zhang, Yi Zhang, Ayibaiheremu Mutailifu, Daoyuan Zhang. Heterologous Overexpression of Desiccation-tolerance Moss ScABI3 Gene Changes Stomatal Phenotype and Improves Drought Resistance in Transgenic Arabidopsis[J]. Chinese Bulletin of Botany, 2021, 56(4): 414-421.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB20212

https://www.chinbullbotany.com/EN/Y2021/V56/I4/414

Figures/Tables 8

Figure 1 PCR test of ScABI3 overexpression transgenic Arabidopsis M: DL2000 marker; 1-6: Transgenic lines; 7: Positive control; 8: Wild type

Figure 2 Expression level of ScABI3 in leave of Arabidopsis transgenic lines ** indicate extremely significant differences at P<0.01.

Figure 3 Stomata phenotype of the transgenic Arabidopsis and wild type (WT) under mannitol treatment Bars=2 μm

Figure 4 Stomata length (A) and width (B) of the transgenic Arabidopsis and wild type (WT) under mannitol treatment * indicate significant differences at P<0.05, ** indicate extremely significant differences at P<0.01.

Figure 5 The stomatal density and water use efficiency (WUE) in the transgenic Arabidopsis and wild type (WT) (A) Stomatal number of transgenic (left) Arabidopsis and WT (right); (B) Stomatal number statistics of the transgenic Arabidopsis and WT; (C) WUE of transgenic Arabidopsis and WT under mannitol treatment. The red arrows indicate stomatas; * indicate significant differences at P<0.05, ** indicate extremely significant differences at P<0.01. (A) Bars=10 μm

Figure 6 The content of ABA in the transgenic Arabidopsis and wild type (WT) under mannitol treatment ** indicate extremely significant differences at P<0.01.

Figure 7 Comparisons of the transgenic Arabidopsis and wild type (WT) under drought stress Phenotype (A), survival rate (B), water loss rate (C) and the phenotype of water loss to detached leaves (D) of the transgenic plant and WT under drought stress. * indicate significant differences at P<0.05. (A) Bars=2 cm; (D) Bars=10 mm

Figure 8 Physiological indicators of the transgenic Arabidopsis and wild type (WT) under mannitol stress (A) Superoxide dismutase (SOD) activity; (B) Peroxidase (POD) activity; (C) H2O2 contents; (D) Malondialdehyde (MDA) contents. * indicate significant differences at P<0.05, ** indicate extremely significant differences at P<0.01.

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