Advances in Extraction and Analysis Technologies of Plant Carotenoids

doi:10.11983/CBB18091

Abstract

Abstract: Carotenoids are lipophilic natural pigments, which synthesized by all photosynthetic organisms and some non-photosynthetic prokaryotes and fungi, and have a wide range of biological effects on plants, animals and humans. With the rapid development of modern analytical techniques, more and more carotenoids will be discovered and utilized. This article systematically elaborated the latest research progresses in the extraction, isolation and purification, content determination and structure identification of plant carotenoids in order to provide technical support for effective development and utilization of plant carotenoids.

Key words: carotenoid, extraction, isolation and purification, content determination, structure identification

He Qing, Sun Guofeng, Lin Qinwen, Li Xiaodong, Zhang Jinzheng. Advances in Extraction and Analysis Technologies of Plant Carotenoids[J]. Chinese Bulletin of Botany, 2018, 53(5): 700-709.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB18091

https://www.chinbullbotany.com/EN/Y2018/V53/I5/700

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References 76

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提取方法	优点	缺点
溶剂提取	高提取率, 设备简单	需要大量有机溶剂且成本较高
绿色溶剂提取	环境友好, 可直接用于食品配方	成本较高且不适合分析鉴定
酶辅助提取	快速, 高效且溶剂用量少	酶的成本高
微波辅助提取	简便, 快速且经济	会导致类胡萝卜素的热降解和顺反异构化
超声波辅助提取	快速, 非热且高效	超声波探头表面老化会改变萃取效率
超临界流体萃取	溶剂非易燃、无毒且可循环利用(CO₂和乙醇); 连续萃取代替间歇处理; 可用于萃取热分子化合物; 可提供高纯度的类胡萝卜素	不适合含有大量水分的样品, 极性类胡萝卜素产率低且仪器成本高

提取方法	优点	缺点
溶剂提取	高提取率, 设备简单	需要大量有机溶剂且成本较高
绿色溶剂提取	环境友好, 可直接用于食品配方	成本较高且不适合分析鉴定
酶辅助提取	快速, 高效且溶剂用量少	酶的成本高
微波辅助提取	简便, 快速且经济	会导致类胡萝卜素的热降解和顺反异构化
超声波辅助提取	快速, 非热且高效	超声波探头表面老化会改变萃取效率
超临界流体萃取	溶剂非易燃、无毒且可循环利用(CO₂和乙醇); 连续萃取代替间歇处理; 可用于萃取热分子化合物; 可提供高纯度的类胡萝卜素	不适合含有大量水分的样品, 极性类胡萝卜素产率低且仪器成本高

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