Chinese Bulletin of Botany ›› 2019, Vol. 54 ›› Issue (3): 371-377.DOI: 10.11983/CBB18134
• TECHNIQUES AND METHODS • Previous Articles Next Articles
Quandong Nong1,2,3,Mingyong Zhang1,2,Mei Zhang2,Shuguang Jian2,Hongfang Lu2,Kuaifei Xia1,2,*,Heming Wen3,*
Received:
2018-06-11
Accepted:
2018-10-14
Online:
2019-07-01
Published:
2019-11-24
Contact:
Kuaifei Xia,Heming Wen
Quandong Nong,Mingyong Zhang,Mei Zhang,Shuguang Jian,Hongfang Lu,Kuaifei Xia,Heming Wen. Improvement in Extraction Methods of Genomic DNA from Pitaya Stems[J]. Chinese Bulletin of Botany, 2019, 54(3): 371-377.
Figure 1 The washed samples of pitaya mixed with CTAB lysate 1-3: Represented the precipitation can not be mixed with CTAB lysate; 4-9: Represented the precipitation can be mixed with CTAB lysate
No. | Improved 1 | Improved 2 | Improved 3 | |||
---|---|---|---|---|---|---|
OD260/OD280 | OD260/OD230 | OD260/OD280 | OD260/OD230 | OD260/OD280 | OD260/OD230 | |
1 | 1.99 | 1.38 | 3.83 | 0.48 | 2.06 | 1.51 |
2 | 1.90 | 1.16 | 2.12 | 0.92 | 2.04 | 1.79 |
3 | 2.03 | 1.30 | 2.69 | 0.63 | 2.05 | 1.65 |
Table 1 The OD260/OD280 and OD260/OD230 of DNA extracted from pitaya by 3 different improved CTAB+Tris-HCl washing methods
No. | Improved 1 | Improved 2 | Improved 3 | |||
---|---|---|---|---|---|---|
OD260/OD280 | OD260/OD230 | OD260/OD280 | OD260/OD230 | OD260/OD280 | OD260/OD230 | |
1 | 1.99 | 1.38 | 3.83 | 0.48 | 2.06 | 1.51 |
2 | 1.90 | 1.16 | 2.12 | 0.92 | 2.04 | 1.79 |
3 | 2.03 | 1.30 | 2.69 | 0.63 | 2.05 | 1.65 |
Figure 2 Electrophoretograms of pitaya DNA, enzyme digestion and ISSR amplification (A) Electrophoretogram of DNA extracted by 3 different improved methods (M: DNA marker; Lane 1-3: DNA extracted by improved 1 method; Lane 4-6: DNA extracted by improved 2 method; Lane 7-9: DNA extracted by improved 3 method). (B) Electrophoretogram of pitaya DNA extracted by improved 3 method and enzyme digestion (M: DNA marker; Lane 1-7: DNA; Lane 8-14: DNA digested by HindIII; Lane 15-21: DNA digested by HindIII and EcoRI; The DNA template of 8-14 and 15-21 is corresponding to the DNA in 1-7 lanes, respectively). (C) Electrophoretogram of ISSR amplification (M: DNA marker; Lane 1-7: Products of 7 different DNA templates ISSR amplification).
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