Chinese Bulletin of Botany ›› 2016, Vol. 51 ›› Issue (1): 68-73.DOI: 10.11983/CBB15057

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A Rapid and Nondestructive Method for Soybean DNA Extraction and Its Application

Wen Cheng1,2, Zhengjun Xia3, Xianzhong Feng2, Suxin Yang2*   

  1. 1College of Life Sciences, Shandong Normal University, Jinan 250014, China
    2Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China
    3Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081, China
  • Received:2015-03-31 Accepted:2015-09-25 Online:2016-01-01 Published:2016-02-01
  • Contact: Yang Suxin
  • About author:? These authors contributed equally to this paper

Abstract:

Genotyping is an important work in plant genetic analysis and molecular marker-assisted breeding. This study established a simple method for genotyping soybean from mature seeds. By this method, a skilled worker can perform the sample collection and DNA extraction of 120 samples from soybean seeds in 1 h and the treated seeds can germinate normally. The DNA obtained by this method can meet the requirement for PCR amplification, and the success rate of PCR reaction is over 98%. This method can be widely used for identifying soybean hybrid seeds, seed purity and genotyping.

Key words: soybean seed, DNA extraction, nondestructive genotyping