耐寒睡莲花瓣中花青素苷组成及其与花色的关系

doi:10.3724/SP.J.1259.2012.00437

植物学报 ›› 2012, Vol. 47 ›› Issue (5): 437-453.DOI: 10.3724/SP.J.1259.2012.00437 cstr: 32102.14.SP.J.1259.2012.00437

• 研究论文 • 下一篇

耐寒睡莲花瓣中花青素苷组成及其与花色的关系

朱满兰^1,2, 王亮生^2*, 张会金², 徐彦军³, 郑绪辰^1,2, 王丽金^2,4

¹南京农业大学园艺学院, 南京 210095;
²中国科学院植物研究所北京植物园/资源植物研发重点实验室, 北京 100093
³中国农业大学理学院, 北京 100094;
⁴中国科学院研究生院, 北京 100049

收稿日期:2012-03-30 修回日期:2012-05-17 出版日期:2012-09-01 发布日期:2012-07-24
通讯作者: 王亮生

Relationship Between the Composition of Anthocyanins and Flower Color Variation in Hardy Water Lily (Nymphaea spp.) Cultivars

Manlan Zhu^1,2, Liangsheng Wang^2*, Huijin Zhang², Yanjun Xu³, Xuchen Zheng^1,2, Lijin Wang^2,4

¹College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China;

²Beijing Botanical Garden/Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;

³Department of Applied Chemistry, China Agricultural University, Beijing 100094, China;

⁴Graduate University of the Chinese Academy of Sciences, Beijing 100049, China

Received:2012-03-30 Revised:2012-05-17 Online:2012-09-01 Published:2012-07-24
Contact: Liangsheng Wang

摘要/Abstract

摘要： 睡莲(Nymphaea spp.)为多年生水生观赏花卉。以耐寒睡莲不同花色的119个栽培品种为材料, 利用高效液相色谱(HPLC-DAD)和液质联用技术(HPLC-ESI-MSⁿ)测定了其花瓣中的花青素苷成分。采用英国皇家园艺学会比色卡(RHSCC)和国际照明委员会(CIE)制定的CIEL^*a^*b^*表色系统测量了57个品种的花色, 运用多元线性回归方法分析花色与花青素苷组成之间的关系。结果表明: 耐寒睡莲花瓣中含有14种花青素苷, 其中飞燕草素-3-半乳糖-5-乙酰-半乳糖苷(Dp3Ga5acetylGa)、飞燕草素-3-鼠李糖-(1→2)-半乳糖苷(Dp3Rh(1→2)Ga)、矢车菊素-3-半乳糖-(1→2)-半乳糖苷(Cy3Ga(1→2)Ga)、矢车菊素-3-乙酰-半乳糖-(1→2)-半乳糖苷(Cy3acetylGa(1→2)Ga)、矢车菊素-3-没食子酰-半乳糖苷(Cy3galloylGa)、飞燕草素-3-乙酰-葡萄糖苷(Dp3acetylG)、飞燕草素-3-葡萄糖苷(Dp3G)和矢车菊素-3-半乳糖-半乳糖-半乳糖苷(Cy3GaGaGa)8个组分在耐寒睡莲中为首次报道。Dp3Ga、Dp3galloylGa、Cy3Ga(1→2)Ga和Cy3galloylGa是决定耐寒睡莲呈色的关键花青素苷。

关键词: 花青素苷, 花色, 耐寒睡莲, 高效液相色谱, 液质联用

Abstract: Water lily (Nymphaea spp.) is a perennial aquatic flower plant. We investigated color of 57 hardy water lily cultivars by the Royal Horticultural Society Colour Chart (RHSCC) and International Commission on Illumination CIEL^*a^*b^* scale. High-performance liquid chromatography (HPLC) with photodiode array detection (DAD) and HPLC electrospray ionization mass spectrometry (HPLC-ESI-MSⁿ) identified 14 anthocyanins. Eight compounds were identified in the petals of 119 hardy water lily cultivars for the first time. Multiple linear regression revealed that Dp3Ga, Dp3galloylGa, Cy3Ga(1→2)Ga and Cy3galloylGa compounds were key pigments in petal color. We discuss differences in anthocyanin components between hardy and tropical water lily and blue color breeding of hardy water lily.

Key words: anthocyanin, flower color, hardy water lily, HPLC-DAD, HPLC-ESI-MSⁿ

朱满兰, 王亮生, 张会金, 徐彦军, 郑绪辰, 王丽金. 耐寒睡莲花瓣中花青素苷组成及其与花色的关系. 植物学报, 2012, 47(5): 437-453.

Manlan Zhu, Liangsheng Wang, Huijin Zhang, Yanjun Xu, Xuchen Zheng, Lijin Wang. Relationship Between the Composition of Anthocyanins and Flower Color Variation in Hardy Water Lily (Nymphaea spp.) Cultivars. Chinese Bulletin of Botany, 2012, 47(5): 437-453.

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耐寒睡莲花瓣中花青素苷组成及其与花色的关系

Relationship Between the Composition of Anthocyanins and Flower Color Variation in Hardy Water Lily (Nymphaea spp.) Cultivars

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