植物学报 ›› 2015, Vol. 50 ›› Issue (6): 721-732.DOI: 10.11983/CBB14194

• 研究报告 • 上一篇    下一篇

荷花花粉的类黄酮组成

吴倩1, 邵帅1, 李珊珊2,3, 张会金2, 王亮生2,,A;*()   

  1. 1南京农业大学园艺学院, 南京 210095
    2中国科学院北方资源植物重点实验室/中国科学院植物研究所北京植物园, 北京 100093
    3中国科学院大学, 北京 100049
  • 收稿日期:2014-11-15 接受日期:2015-04-26 出版日期:2015-11-01 发布日期:2015-09-06
  • 通讯作者: 王亮生
  • 作者简介:

    ? 共同第一作者

  • 基金资助:
    国家自然科学基金(No.31471910)

Composition of Flavonoids in Lotus Pollen

Qian Wu1, Shuai Shao1, Shanshan Li2,3, Huijin Zhang2, Liangsheng Wang2*   

  1. 1College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
    2Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
    3University of the Chinese Academy of Sciences, Beijing 100049, China
  • Received:2014-11-15 Accepted:2015-04-26 Online:2015-11-01 Published:2015-09-06
  • Contact: Wang Liangsheng
  • About author:

    ? These authors contributed equally to this paper

摘要:

利用超高效液相色谱质谱联用(I-Class UPLC/Xevo TQ MS)技术, 对50个品种荷花干燥花粉中的类黄酮代谢产物进行了分离及结构鉴定。结果表明, 在荷花(Nelumbo nucifera)花粉中检测到了13种黄酮醇和2种黄酮, 这15种类黄酮化合物均为首次从荷花花粉中检出, 其中槲皮素3-O-葡萄糖醛酸苷(quercetin 3-O-glucuronide)、槲皮素3-O-新橙皮糖苷(quercetin 3-O-rhamnopyranosyl-(1→2)-glucopyranoside)以及槲皮素3-O-阿拉伯糖-(1→2)-半乳糖苷(quercetin 3-O-arabinopy- ranosyl-(1→2)- galactopyranoside)含量较高, 且在所有品种中均有检出。不同品种花粉中检测到的类黄酮总含量(TF)差异较大, 绯云千叶类黄酮总含量最高, 为281.08 mg∙100 g-1; 仙女散花最低, 仅为82.64 mg∙100 g-1。通过聚类分析, 将50个品种聚为4组, B组类黄酮化合物种类最多, 而且该组总类黄酮含量最高, 其中绯云千叶、伯里小姐和蜀红莲的干燥花粉中总类黄酮含量均超过200 mg∙100 g-1, 可推荐为采集荷花花粉用的优良品种。

Abstract:

We used ultra-performance liquid chromatography-mass spectrometry (I-Class UPLC/Xevo TQ MS) to separate and identify the flavonoids in lotus pollens from 50 cultivars. Lotus pollen contains 13 flavonols and 2 flavones, all reported in lotus pollen for the first time. The most abundant flavonoids were quercetin glucosides, namely quercetin 3-O-glucuronide, quercetin 3-O-rhamnopyranosyl-(1→2)-glucopyranoside and quercetin 3-O-arabinopyranosyl-(1→2)- galactopyranoside, detected in all cultivar pollen. The total flavonoid (TF) content ranged from 82.64 to 281.08 mg∙100 g-1 in 50 cultivars, with Fei-Yun-Qian-Ye showing the highest TF and Xian-Nv-San-Hua the lowest. According to cluster analysis, 50 lotus cultivars were classified into four groups; group B contained more flavonoids than other groups, and group B TF content was the highest. TF content in Fei-Yun-Qian-Ye, Bo-Li-Xiao-Jie and Shu-Hong-Lian exceeded 200 mg∙100 g-1 dry weight and could be developed into lotus pollen products.