植物学报 ›› 2018, Vol. 53 ›› Issue (4): 477-486.DOI: 10.11983/CBB17112

• 研究报告 • 上一篇    下一篇

云南疣粒野生稻稻瘟病抗性

王韵茜, 苏延红, 杨睿, 李鑫, 李晶, 曾千春*(), 罗琼*()   

  1. 云南农业大学, 农业生物多样性与病虫害控制教育部重点实验室, 云南生物资源保护与利用国家重点实验室, 昆明 650201
  • 收稿日期:2017-06-05 接受日期:2017-08-07 出版日期:2018-07-01 发布日期:2018-09-11
  • 通讯作者: 曾千春,罗琼
  • 作者简介:† 共同第一作者。
  • 基金资助:
    国家重点研发计划(No.2016YFD0100600)和国家自然科学基金-云南联合基金(No.U1302261)

Rice Blast Resistance of Wild Rice in Yunnan

Wang Yunqian, Su Yanhong, Yang Rui, Li Xin, Li Jing, Zeng Qianchun*(), Luo Qiong*()   

  1. State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Ministry of Education Key Laboratory of Agriculture Biodiversity for Plant Disease Management, Yunnan Agricultural University, Kunming 650201, China
  • Received:2017-06-05 Accepted:2017-08-07 Online:2018-07-01 Published:2018-09-11
  • Contact: Zeng Qianchun,Luo Qiong
  • About author:† These authors contributed equally to this paper

摘要: 野生稻(Oryza rufipogo)保存有许多栽培稻(O. sativa)不具备或已经消失的优异基因资源, 是扩大栽培稻遗传背景、改良产量与品质、提高抗病虫害及抗逆境能力的重要基因库。疣粒野生稻(O. meyeriana)是中国3种野生稻资源之一, 主要分布在云南。为进一步了解其稻瘟病抗性, 首先利用来自不同稻作区的稻瘟病菌株, 通过注射接种法对疣粒野生稻进行系统的稻瘟病抗性鉴定, 发现疣粒野生稻对接种的所有稻瘟病菌株都感病。进一步采用3'/5' RACE方法, 从疣粒野生稻中克隆了水稻同源基因Pid2Pid3, 并构建过表达转基因株系对基因功能进行了研究。结果表明, Pid2Pid3与疣粒野生稻中同源基因间在DNA和氨基酸水平上有较大的序列差异, 过表达转基因的日本晴植株对稻瘟病菌的敏感性与对照相似。推测疣粒野生稻在自然接种条件下, 表现出的抗稻瘟病表型很可能是其旱生叶片结构特征形成了对稻瘟病菌侵染的天然屏障。对控制疣粒野生稻这一类性状基因资源的挖掘和利用, 有利于优良抗性水稻品种的培育。研究结果为疣粒野生稻的研究利用提供了新信息和新思路。

关键词: 疣粒野生稻, 稻瘟病抗性, 抗病基因, 基因克隆, 稻瘟病菌

Abstract: Wild species of Oryza contain numerous genes of economic importance and are being used as an important cultivated rice gene pool to expand the genetic background, improve yield and quality, and increase the ability for resistance or tolerance to pests as well as bioic and abiotic stresses. O. meyeriana is one of three native wild rice species in China, mainly distributed in Yunnan province. To further understand the disease resistance to rice blast, we performed a systematic investigation of rice blast resistance with O. meyeriana. O. meyeriana plants were inoculated by injection with multiple blast strains from different rice planting areas. Then, we cloned and functionally identified Pid2 and Pid3 orthologous genes in O. meyeriana. O. meyeriana was sensitive to all blast isolates used in this experiment. The DNA and amino acid sequences significantly differed between Pid2 and its orthologs in O. meyeriana, as did Pid3 and its orthologs. Nipponbare plants overexpressing the orthologous genes of Pid2 and Pid3 showed mildly enhanced susceptibility to Magnaporthe oryzae. The orthologs of blast-resistant genes in O. meyeriana may be susceptible alleles. O. meyeriana exhibited resistance to rice blast under natural inoculation likely due to a xeromorphic leaf structure that reduces susceptibility to M. oryzae. Characterization of these genes will help breed elite rice. Our study provides new information and ideas for research and utilization of O. meyeriana.

Key words: wild rice (Oryza meyeriana), rice blast resistance, R gene, gene cloning, Magnaporthe oryzae