植物学报 ›› 2015, Vol. 50 ›› Issue (2): 171-179.DOI: 10.3724/SP.J.1259.2015.00171

• 研究报告 • 上一篇    下一篇

小立碗藓对重金属镉胁迫的应答特征

伍自力1,2, 余孟瑶2, 陈露2, 魏静2, 王晓琴3, 胡勇2, 闫妍2, 万平2,*()   

  1. 1宜宾学院生命科学与食品工程学院, 香料植物资源开发与利用四川省高校重点实验室, 宜宾 644000
    2首都师范大学植物资源与低碳环境北京市重点实验室, 北京 100048
    3北京农学院农业部都市农业(北方)重点实验室, 北京 102206
  • 收稿日期:2014-10-27 接受日期:2015-02-02 出版日期:2015-03-01 发布日期:2015-04-10
  • 通讯作者: 万平
  • 作者简介:

    ? 共同第一作者

  • 基金资助:
    转基因生物新品种培育重大专项(No.2013ZX08001003-008)和首都师范大学植物学国家重点学科

Transcriptome Analysis of Physcomitrella patens Response to Cadmium Stress by Bayesian Network

Zili Wu1, 2, Mengyao Yu2, Lu Chen2, Jing Wei2, Xiaoqin Wang3, Yong Hu2, Yan Yan2, Ping Wan2, *   

  1. 1Key Laboratory of Aromatic Plant Resources Exploitation and Utilization in Sichuan Higher Education, College of Life Sciences and Food Engineering, Yibin University, Yibin 644000, China
    2Beijing Key Laboratory of Plant Gene Resource and Low-carbon Environmental Biotechnology, Capital Normal University, Beijing 100048, China
    3Key Laboratory of Urban Agriculture (North) Ministry of Agriculture, Beijing University of Agriculture, Beijing 102206, China
  • Received:2014-10-27 Accepted:2015-02-02 Online:2015-03-01 Published:2015-04-10
  • Contact: Wan Ping
  • About author:

    ? These authors contributed equally to this paper

摘要: 镉是植物非必需的微量重金属元素, 镉胁迫引起植物细胞的代谢紊乱, 甚至导致细胞死亡。为了探索苔藓植物对镉胁迫的应答机制, 采用高通量测序及生物信息学技术分析了藓类模式植物——小立碗藓(Physcomitrella patens)在镉胁迫下的基因表达特征。结果表明, 在镉胁迫下, 小立碗藓细胞骨架组织、微管运动、DNA修复系统、端粒维护、配子体形成与有性生殖以及与氮代谢等相关基因的表达具有明显的镉胁迫应答特征, 暗示了这些基因可能共同参与小立碗藓对镉胁迫的调控反应。该研究结果为阐明植物对镉胁迫的应答机制提供了新的线索。

Abstract: Cadmium is a non-essential heavy metal for plant growth. Cadmium stress causes cell metabolism disturbance or death in plant. Here, we performed transcriptome analysis of Physcomitrella patens during cadmium stress by RNA-Seq. We revealed a new transcriptional network of cadmium stress in plants. The functions of genes that were upregulated or downregulated under cadmium stress included microtubule-based movement, microtubule-based processing, cytoskeleton organization, DNA replication, DNA metabolic process, telomere maintenance and organization, sexual reproduction, urea metabolic process, and nitrogen cycle metabolic process. These proteins may play roles in P. patens under cadmium stress. Our study provides new information for the further research of the molecular mechanisms of plant adaptation to cadmium stress.