植物学报 ›› 2005, Vol. 22 ›› Issue (增刊): 82-90.

• 专题介绍 • 上一篇    下一篇

作物分子标记辅助选择的研究进展、影响因素及其发展策略

刘志文 傅廷栋 刘雪平 涂金星 陈宝元   

  1. (华中农业大学作物遗传改良国家重点实验室, 国家油菜改良中心武汉分中心 武汉 430070)
  • 收稿日期:2004-07-12 修回日期:2005-01-10 出版日期:2005-08-10 发布日期:2005-08-10
  • 通讯作者: 傅廷栋
  • 基金资助:
    国家 863 计划项目(2003AA2071050)资助

Advances, Influence Factors and Development Strategies in Crop Molecular-assisted Selection

LIU Zhi-Wen FU Ting-Dong LIU Xue-Ping TU Jin-Xing CHEN Bao-Yuan   

  1. (National Key Laboratory of Crop Genetic Improvement , National Center of Rapeseed Genetic Improvement (Wuhan) , Huazhong Agricultural University , Wuhan 430070)
  • Received:2004-07-12 Revised:2005-01-10 Online:2005-08-10 Published:2005-08-10

摘要: 随着分子标记技术及其检测手段的发展, 开发和应用成本的降低, 分子标记辅助选择(MAS)在作物育种上的应用优势日益明显。本文综述了近年来MAS在基因聚合、基因转移和数量性状改良上的研究进展。总结了MAS的影响因素, 包括标记与基因间的距离、目标性状的遗传率、群体大小、所用分子标记的数目、类型和相位等。并提出育种和定位同步进行、选择合适分子标记类型和数量、简化DNA 提取方法、背景选择的逐步选择法、确定合适选择方案等MAS发展策略。

关键词: 分子标记辅助选择, 作物育种, 质量性状, 数量性状

Abstract: With the development of molecular marker technologies and detection techniques and the decrease in the exploitation and application cost, molecular-assisted selection (MAS) shows superior applications in crop breeding. This paper reviews the advances in gene pyramiding, gene transfer and quantity trait improvements with MAS and summarizes the influence factors, such as the distance between the gene of interest and markers, trait heredity, individual number of breeding population, and marker number, type and phase. As a result, we propose that strategies for crop breeding with MAS include mapping and breeding at the same time, determining molecular marker type and number, simplifying the DNA extraction methods, and stepwise selection of background selection with a suitable scheme.

Key words: Molecular-assisted selection (MAS), Crop breeding, Quality trait, Quantitative trait loci