牡丹TCP基因家族鉴定及TCP15a功能分析

doi:10.11983/CBB25005

植物学报

牡丹TCP基因家族鉴定及TCP15a功能分析

韩新蕊^{1, 2}, 袁欣², 高杰², 王亮生³, 王晓晖⁴, 贾文庆¹*, 符真珠²*, 张和臣²*

1河南科技学院, 新乡 453003; 2河南省农业科学院园艺研究所, 郑州 450002; 3中国科学院植物研究所, 北京 100093; 4洛阳市农林科学院, 洛阳 471026

收稿日期:2025-01-15 修回日期:2025-03-13 出版日期:2025-03-18 发布日期:2025-03-18
通讯作者: 贾文庆, 符真珠, 张和臣
基金资助:
国家自然科学基金(No.32030095)、河南省联合基金(No.242301420128)、河南省农业科学院创新团队项目(No.2024TD36)、河南省科技攻关项目“切花牡丹核心种质资源构建及应用”(No.242102110269)和中央引导计划项目(No.Z20241471128)

Identification of TCP Gene Family and Functional Analysis of TCP15a of Tree Peony

Xinrui Han^{1, 2}, Xin Yuan², Jie Gao², Liangsheng Wang³, Xiaohui Wang⁴, Wenqing Jia^1*, Zhenzhu Fu^2*, Hechen Zhang^2*

¹Henan Institute of Science and Technology, Xinxiang 453003, China; ²Horticultural Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; ³Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; ⁴Luoyang Academy of Agricultural and Forestry Sciences, Luoyang 471026, China

Received:2025-01-15 Revised:2025-03-13 Online:2025-03-18 Published:2025-03-18
Contact: Wenqing Jia, Zhenzhu Fu, Hecheng Zhang

摘要/Abstract

摘要： 为了研究TCP转录因子家族在牡丹(Paeonia sect. Moutan)花瓣呈色过程中的作用, 为牡丹花色改良和分子育种提供候选基因。本研究对牡丹TCP家族进行鉴定、生物信息学分析, 并对可能参与花色调控的基因TCP13、TCP15a进行功能分析。研究结果表明: 在3个牡丹基因组中共鉴定出26个TCP家族成员, 分为2个亚族3个亚类, 不规则的分布在5条染色体上; 不同成员的理化性质、基因结构存在差异, 但均含有TCP保守结构域。矮牵牛(Petunia hybrida)花瓣瞬时表达TCP13、TCP15a, 发现TCP13对MYB57诱导的紫色表型无明显作用, 而TCP15a可显著抑制; 荧光定量PCR分析表明, TCP15a可以显著抑制MYB57诱导浸染的花瓣组织内花青苷合成酶基因CHSa、DFR、ANS的表达。进一步通过蛋白互作鉴定发现, TCP15a与MBW蛋白复合体成员WD40-1、WD40-2互作。TCP15a通过与MYB57竞争与WD40的相互作用, 影响了MBW复合体的形成, 进而抑制了MYB57对花青苷合成酶基因的调控作用。本研究为深入研究牡丹TCP家族基因功能奠定理论基础, 为牡丹花色定向育种提供了有价值的线索。

关键词: 牡丹, 花色, TCP转录因子家族, 功能分析

Abstract: INTRODUCTION: Flower color is one of the important traits to evaluate the ornamental value of tree peony (Paeonia suffruticosa). Anthocyanin plays a major role in the color of tree peony petals. The biosynthesis of anthocyanin is mainly controlled by two types of genes which are directly involved in pigment metabolism and transcription factors which play a regulatory role. TCP transcription factors are involved in the regulation of secondary metabolism, flavonoid and chlorophyll synthesis in plants. Therefore, it is of great value for further analysis of anthocyanin biosynthesis and regulation mechanism to explore the gene function of TCP family in tree peony and clarify the regulation mechanism of TCP in tree peony petal coloration.

RATIONALE: In order to study the role of TCP transcription factor family in the process of tree peony petal coloration and provide candidate genes for tree peony flower color improvement and molecular breeding, the TCP family of tree peony was identified and analyzed by bioinformatics, and the function of genes TCP13 and TCP15a that may be involved in flower color regulation was analyzed.

RESULTS: Twenty-six TCP gene family members were identified from three tree peony genomes and divided into two subtribes and three subclasses, which were irregularly distributed on 5 chromosomes. The physicochemical properties and gene structure of different members were different, but all members contained TCP conserved domains. The transient expression of TCP13 and TCP15a, which may be involved in petal coloration, showed that TCP13 had no obvious effect on the purple phenotype induced by MYB57, while TCP15a could significantly inhibit it.Fluorescence quantitative PCR analysis showed that TCP15a could significantly inhibit the expression of anthocyanin synthase genes CHSa, DFR and ANS in the tissue of disseminated petals. The interaction between TCP15a and MBW protein complex members WD40-1 and WD40-2 was identified by protein interaction identification.

CONCLUSION: In this study, twenty-six TCP gene family members were screened and identified by mining the tree peony genome database, and the characteristics of TCP encoded amino acids, evolutionary relationship and gene structure were analyzed by bioinformatics.It was found that TCP15a may inhibit the biosynthesis of anthocyanins by competing with MYB57 and interacting with WD40, regulating the expression of enzyme genes CHSa, DFR and ANS, etc. The results provided a theoretical basis for exploring the molecular mechanism of tree peony petal coloration.

Identification of TCP gene family and functional analysis of TCP13 and TCP15a of tree peony. In this study, twenty-six TCP gene family members were screened and identified, and the characteristics of TCP encoded amino acids, evolutionary relationship and gene structure were analyzed by bioinformatics. And.TCP15a was found to inhibit anthocyanin biosynthesis by competing with MYB57 and interacting with WD40.

Key words: Tree peony, flower color, TCP transcription factor family, functional analysis

韩新蕊, 袁欣, 高杰, 王亮生, 王晓晖, 贾文庆, 符真珠, 张和臣. 牡丹TCP基因家族鉴定及TCP15a功能分析. 植物学报, DOI: 10.11983/CBB25005.

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牡丹TCP基因家族鉴定及TCP15a功能分析

Identification of TCP Gene Family and Functional Analysis of TCP15a of Tree Peony

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