苦杨×小叶杨杂交F1代苗期抗旱性比较研究

doi:10.11983/CBB22086

植物学报 ›› 2023, Vol. 58 ›› Issue (4): 519-534.DOI: 10.11983/CBB22086 cstr: 32102.14.CBB22086

苦杨×小叶杨杂交F₁代苗期抗旱性比较研究

张蕾¹, 姜鹏飞¹, 王一鸣², 兰婷³, 刘妍婧¹, 曾庆银¹()

1.中国林业科学研究院, 林木遗传育种国家重点实验室, 北京 100091
2.北京林业大学生物科学与技术学院, 北京 100083
3.西南大学生命科学学院, 重庆 400715

收稿日期:2022-04-23 接受日期:2022-06-16 出版日期:2023-07-01 发布日期:2022-07-17
通讯作者: *E-mail: qingyin.zeng@caf.ac.cn
基金资助:
国家重点研发计划(2022YFD2200102);国家自然科学基金(32101552)

Comparative Study on the Drought Resistance of Young Seedling from Populus laurifolia × P. simonii F₁ Progeny

Lei Zhang¹, Pengfei Jiang¹, Yiming Wang², Ting Lan³, Yanjing Liu¹, Qingyin Zeng¹()

1. State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China
2. College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China
3. School of Life Sciences, Southwest University, Chongqing 400715, China

Received:2022-04-23 Accepted:2022-06-16 Online:2023-07-01 Published:2022-07-17
Contact: *E-mail: qingyin.zeng@caf.ac.cn

摘要/Abstract

摘要： 创制抗旱林木新品种对维持我国干旱半干旱地区森林生产力, 应对全球气候变暖具有重要意义。苦杨(Populus laurifolia)是分布在新疆额尔齐斯河流域的我国乡土树种, 具有速生和耐寒等优良特性, 而小叶杨(P. simonii)具有抗旱和耐瘠薄特性。我们对苦杨×小叶杨杂交F₁代幼苗的抗旱性进行全面分析和综合评价。测定了正常生长与干旱胁迫下亲本和杂交F₁代的株高生长量和叶片相对含水量等7个生长指标, 净光合速率和胞间CO₂浓度等6个光合参数, 以及SOD活性和MDA含量等5个抗旱生化指标。对18个性状指标进行抗旱系数和隶属函数分析, 将亲本及23个F₁代幼苗划分为高、中和低3个抗旱类型。高度抗旱型幼苗的叶片、上表皮、下表皮和栅栏组织厚度较大, 叶片组织结构紧密度高, 且在干旱胁迫下抗旱关键基因的表达量显著高于其它抗旱类型幼苗。该研究为杨树抗旱育种提供了理论指导和基础材料。

关键词: 杨树, 杂交, 苗期, 抗旱性, 评价

Abstract: The development of drought-resistant tree varieties is of great significance for maintaining forest productivity in arid and semi-arid regions of China and addressing the challenges of global climate change. Populus laurifolia is distributed in the Irtysh River Basin in Xinjiang, China, has excellent characteristics such as fast growth and cold resistance. P. simonii has the characteristics of drought resistance and barren resistance, and is widely planted in northern China. In this study, the drought resistance characteristics of P. laurifolia × P. simonii F₁ progeny were comprehensively analyzed and evaluated. We investigated seven growth parameters such as high growth and relative leaf water content, six photosynthetic parameters such as net photosynthetic rate and intercellular CO₂ concentration, and five biochemical characteristics such as SOD activity and MDA content. Based on the drought resistance coefficient and membership function analysis of 18 trait parameters, the parent and 23 F₁ progeny were divided into three drought resistance types: high, medium and low. Compared to other two drought resistance types seedlings, the highly drought-resistant F₁ progeny seedlings had larger leaf thickness, upper and lower epidermis thickness, palisade tissue thickness, and leaf tissue compactness. For highly drought-resistant F₁ progeny seedlings, the expression levels of key drought resistance genes under drought stress were significantly higher than other two drought resistance types. This study provides theoretical guidance and material basis for breeding drought-resistant poplar.

Key words: poplar, hybrid, seedling stage, drought resistance, evaluation

张蕾, 姜鹏飞, 王一鸣, 兰婷, 刘妍婧, 曾庆银. 苦杨×小叶杨杂交F₁代苗期抗旱性比较研究. 植物学报, 2023, 58(4): 519-534.

Lei Zhang, Pengfei Jiang, Yiming Wang, Ting Lan, Yanjing Liu, Qingyin Zeng. Comparative Study on the Drought Resistance of Young Seedling from Populus laurifolia × P. simonii F₁ Progeny. Chinese Bulletin of Botany, 2023, 58(4): 519-534.

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB22086

https://www.chinbullbotany.com/CN/Y2023/V58/I4/519

图/表 16

参考文献 26

[1]	陈章水 (2014). 东北华北关中地区杨树栽培新技术. 北京: 金盾出版社. pp. 57-58.
[2]	冯冬霞, 施生锦 (2005). 叶面积测定方法的研究效果初报. 中国农学通报 21(6), 4.
[3]	付士磊, 周永斌, 何兴元, 陈玮 (2006). 干旱胁迫对杨树光合生理指标的影响. 应用生态学报 17, 2016-2019.
[4]	姬慧娟 (2015). 丹红杨与小叶杨杂交子代苗期抗旱相关性状遗传分析. 硕士论文. 北京: 中国林业科学研究院. pp. 20-21.
[5]	刘增兵, 姜景彬, 杨欢欢, 姜秀明, 李景富 (2019). 植物杂种优势的研究进展. 分子植物育种 17, 4127-4134.
[6]	潘静, 韩蕾 (2017). 葡萄叶片叶绿素含量测定方法比较. 西北园艺 (6), 58-60.
[7]	山仑, 邓西平, 康绍忠 (2002). 我国半干旱地区农业用水现状及发展方向. 水利学报 33(9), 27-31.
[8]	孙佩, 姬慧娟, 张亚红, 贾会霞, 胡建军 (2019). 丹红杨×通辽1号杨杂交子代苗期抗旱性初步评价. 植物遗传资源学报 20, 297-308. DOI
[9]	《新疆植物志》编写委员会 (2019). 新疆植物志(第1卷). 乌鲁木齐: 新疆科学技术出版社. pp. 170.
[10]	徐虎智, 孟丙南, 李云, 王先保, 吕保聚, 程新林, 梁润峰 (2006). 影响林木扦插成活率的因素分析. 河南林业科技 26(3), 26-27.
[11]	张龙进, 李桂双, 白成科, 文苗苗, 张志勤 (2012). 山茱萸种质资源数量性状评价及相关性分析. 植物遗传资源学报 13, 655-659. DOI
[12]	Ennajeh M, Vadel AM, Cochard H, Khemira H (2010). Comparative impacts of water stress on the leaf anatomy of a drought-resistant and a drought-sensitive olive cultivar. J Hortic Sci Biotechnol 85, 289-294. DOI URL
[13]	Farooq M, Hussain M, Wahid A, Siddique KHM (2012). Drought stress in plants:an overview. In: Aroca R, ed. Plant Responses to Drought Stress: From Morphological to Molecular Features. Berlin: Springer-Verlag. pp. 1-8.
[14]	Farooq M, Wahid A, Kobayashi N, Fujita D, Basra SMA (2009). Plant drought stress: effects, mechanisms and management. Agron Sustain Dev 29, 185-212. DOI URL
[15]	Farquhar GD, Sharkey TD (1982). Stomatal conductance and photosynthesis. Annu Rev Plant Physiol 33, 317-345. DOI URL
[16]	Fathi A, Tari DB (2016). Effect of drought stress and its mechanism in plants. Int J Life Sci 10, 1-6. DOI URL
[17]	Li S, Lin YCJ, Wang PY, Zhang BF, Li M, Chen S, Shi R, Tunlaya-Anukit S, Liu XY, Wang ZF, Dai XF, Yu J, Zhou CG, Liu BG, Wang JP, Chiang VL, Li W (2019). The AREB1 transcription factor influences histone acetylation to regulate drought responses and tolerance in Populus trichocarpa. Plant Cell 31, 663-686. DOI URL
[18]	Lian CL, Li Q, Yao K, Zhang Y, Meng S, Yin WL, Xia XL (2018). Populus trichocarpa PtNF-YA9, a multifunctional transcription factor, regulates seed germination, abiotic stress, plant growth and development in Arabidopsis. Front Plant Sci 9, 954. DOI URL
[19]	Meng S, Cao Y, Li HG, Bian Z, Wang DL, Lian CL, Yin WL, Xia XL (2019). PeSHN1 regulates water-use efficiency and drought tolerance by modulating wax biosynthesis in poplar. Tree Physiol 39, 1371-1386. DOI PMID
[20]	Orchard KA, Cernusak LA, Hutley LB (2010). Photosynthesis and water-use efficiency of seedlings from northern Australian monsoon forest, savanna and swamp habitats grown in a common garden. Funct Plant Biol 37, 1050-1060. DOI URL
[21]	Ulrich K, Ewald D (2014). Breeding triploid aspen and poplar clones for biomass production. Silvae Genet 63, 47-58.
[22]	Vanlerberghe GC, Martyn GD, Dahal K (2016). Alternative oxidase: a respiratory electron transport chain pathway essential for maintaining photosynthetic performance during drought stress. Physiol Plant 157, 322-337. DOI PMID
[23]	Walter J, Nagy L, Hein R, Rascher U, Beierkuhnlein C, Willner E, Jentsch A (2011). Do plants remember drought? Hints towards a drought-memory in grasses. En- viron Exp Bot 71, 34-40.
[24]	Yang YL, Li HG, Wang J, Wang HL, He F, Su YY, Zhang Y, Feng CH, Niu MX, Li ZH, Liu C, Yin WL, Xia XL (2020). ABF3 enhances drought tolerance via promoting ABA- induced stomatal closure by directly regulating ADF5 in Populus euphratica. J Exp Bot 71, 7270-7285. DOI URL
[25]	Yu DD, Wildhagen H, Tylewicz S, Miskolczi PC, Bhalerao RP, Polle A (2019). Abscisic acid signaling mediates biomass trade-off and allocation in poplar. New Phytol 223, 1192-1203. DOI URL
[26]	Zhu JK (2016). Abiotic stress signaling and responses in plants. Cell 167, 313-324. DOI URL

Gene	Primer name	Primer sequences (5′-3′)
Actin	RT-Actin-F	TGTTGCCCTTGACTATGAGCAG- GA
	RT-Actin-R	ACGGAATCTCTCAGCTCCAATG- GT
NAC006	RT-NAC006-F	AAGAACAGCATCTTAGCGTCAAG
	RT-NAC006-R	TGGCGGCAGCAAAACAACCTG
NAC007	RT-NAC007-F	ATGAAAGGAAATAGATCAGCAG- AT
	RT-NAC007-R	ATTGGCCTCCACATTTCTTAAGC
NAC120	RT-NAC120-F	TGTGCGCTAAGCTGCAGTCTG
	RT-NAC120-R	ACCAGCAACTTTCCTGCACAAAT
AREB1-2	RT-AREB1-2-F	CCTAAGCAGCCTAATATGGGATA
	RT-AREB1-2-R	GGCAAATTAGAAGATTGCAAAG- AT
ABF3	RT-ABF3-F	ACTGCCGAAGAGACTCAAGC
	RT-ABF3-R	ACTCCCATCTTCAGCAGCAC
AREB3	RT-AREB3-F	AGGAGGCAGAAGAGGATGAT
	RT-AREB3-R	GTTTCCTCAGCCTTTCATTTTC
SHN1	RT-SHN1-F	AGAGCCTACGATGAAGCAGC
	RT-SHN1-R	ACGAGGAGGAGGGAGAGTTC
NF-YA9	RT-NA9-F	TCAAGTCTCGGAAGCCATACT
	RT-NA9-R	TTGTCATCCAAGGAAGCAAT

Gene	Primer name	Primer sequences (5′-3′)
Actin	RT-Actin-F	TGTTGCCCTTGACTATGAGCAG- GA
	RT-Actin-R	ACGGAATCTCTCAGCTCCAATG- GT
NAC006	RT-NAC006-F	AAGAACAGCATCTTAGCGTCAAG
	RT-NAC006-R	TGGCGGCAGCAAAACAACCTG
NAC007	RT-NAC007-F	ATGAAAGGAAATAGATCAGCAG- AT
	RT-NAC007-R	ATTGGCCTCCACATTTCTTAAGC
NAC120	RT-NAC120-F	TGTGCGCTAAGCTGCAGTCTG
	RT-NAC120-R	ACCAGCAACTTTCCTGCACAAAT
AREB1-2	RT-AREB1-2-F	CCTAAGCAGCCTAATATGGGATA
	RT-AREB1-2-R	GGCAAATTAGAAGATTGCAAAG- AT
ABF3	RT-ABF3-F	ACTGCCGAAGAGACTCAAGC
	RT-ABF3-R	ACTCCCATCTTCAGCAGCAC
AREB3	RT-AREB3-F	AGGAGGCAGAAGAGGATGAT
	RT-AREB3-R	GTTTCCTCAGCCTTTCATTTTC
SHN1	RT-SHN1-F	AGAGCCTACGATGAAGCAGC
	RT-SHN1-R	ACGAGGAGGAGGGAGAGTTC
NF-YA9	RT-NA9-F	TCAAGTCTCGGAAGCCATACT
	RT-NA9-R	TTGTCATCCAAGGAAGCAAT

Drought stress time	Levels
Drought stress time	First level	Second level	Third level	Fourth level
The 5^th day	B13, B8
The 6^th day	B13, B8, B25
The 7^th day	B13, B25	B8
The 8^th day	B25, B17	B13, B8
The 9^th day	B12, C11, B25, B17	B8, B13
The 10^th day	C10, B20, B12, C11, B25	B8, B13, B17
The 11^th day	A10, C10, B20, C6, C25, B12, C11, A12	B17, B25, B13	B8
The 12^th day	B18, B15, A24, C7, A25, B16, B11, B5, B19, A10, Populus laurifolia	C10, B20, C6, C25, B12, C11, A12	B17, B8, B25, B13
The 13^th day	B18, B15, A24, C7, A25, B16, B11, B5, B19, A10, P. laurifolia	C10, B20, C6, C25, B12, C11, A12	B17, B8, B25, B13
The 14^th day	A11, A15, B18, B15, A24, C7, A25, B16, B11, B5, P. laurifolia	B19, A10, C10, B20, C6, C25, B12	C11, A12, B17, B8, B25, B13
The 15^th day	A11, A15, B18, B15, A24, C7, A25, P. laurifolia	B16, B11, B5, B19, A10, C10	B20, C6, C25, B12, C11, A12, B17, B8	B25, B13
The 16^th day	A11, A15, B18, B15, P. laurifolia	A24, C7, A25, B16, B11, B5	B19, A10, C10, B20, C6, C25, B12	C11, A12, B17, B8, B25, B13
The 17^th day	A11, A15, B18	B15, A24, C7, A25, B16, P. laurifolia	B11, B5, B19, A10, C10, B20, C6	C25, B12, C11, A12, B17, B8, B25, B13
The 18^th day	A11, A15	B18, B15, A24, C7, P. laurifolia	A25, B16, B11, B5, B19, A10	C10, B20, C6, C25, B12, C11, A12, B17, B8, B25, B13
The 19^th day		A11, A15, B18, B15, P. laurifolia	A25, A24, C7, B16, B11, B5, B19, A10, B20, C25	C10, C6, B12, C11, A12, B17, B8, B25, B13
The 20^th day		A11, A15, B15	B18, A25, A24, C7, B16, B5, B19, P. laurifolia	B11, A10, C10, B20, C6, C25, B12, C11, A12, B17, B8, B25, B13
The 21^th day		A11, A15	B18, B15, A25, A24, C7, B16, P. laurifolia	B11, B5, B19, A10, C10, B20, C6, C25, B12, C11, A12, B17, B8, B25, B13

Drought stress time	Levels
Drought stress time	First level	Second level	Third level	Fourth level
The 5^th day	B13, B8
The 6^th day	B13, B8, B25
The 7^th day	B13, B25	B8
The 8^th day	B25, B17	B13, B8
The 9^th day	B12, C11, B25, B17	B8, B13
The 10^th day	C10, B20, B12, C11, B25	B8, B13, B17
The 11^th day	A10, C10, B20, C6, C25, B12, C11, A12	B17, B25, B13	B8
The 12^th day	B18, B15, A24, C7, A25, B16, B11, B5, B19, A10, Populus laurifolia	C10, B20, C6, C25, B12, C11, A12	B17, B8, B25, B13
The 13^th day	B18, B15, A24, C7, A25, B16, B11, B5, B19, A10, P. laurifolia	C10, B20, C6, C25, B12, C11, A12	B17, B8, B25, B13
The 14^th day	A11, A15, B18, B15, A24, C7, A25, B16, B11, B5, P. laurifolia	B19, A10, C10, B20, C6, C25, B12	C11, A12, B17, B8, B25, B13
The 15^th day	A11, A15, B18, B15, A24, C7, A25, P. laurifolia	B16, B11, B5, B19, A10, C10	B20, C6, C25, B12, C11, A12, B17, B8	B25, B13
The 16^th day	A11, A15, B18, B15, P. laurifolia	A24, C7, A25, B16, B11, B5	B19, A10, C10, B20, C6, C25, B12	C11, A12, B17, B8, B25, B13
The 17^th day	A11, A15, B18	B15, A24, C7, A25, B16, P. laurifolia	B11, B5, B19, A10, C10, B20, C6	C25, B12, C11, A12, B17, B8, B25, B13
The 18^th day	A11, A15	B18, B15, A24, C7, P. laurifolia	A25, B16, B11, B5, B19, A10	C10, B20, C6, C25, B12, C11, A12, B17, B8, B25, B13
The 19^th day		A11, A15, B18, B15, P. laurifolia	A25, A24, C7, B16, B11, B5, B19, A10, B20, C25	C10, C6, B12, C11, A12, B17, B8, B25, B13
The 20^th day		A11, A15, B15	B18, A25, A24, C7, B16, B5, B19, P. laurifolia	B11, A10, C10, B20, C6, C25, B12, C11, A12, B17, B8, B25, B13
The 21^th day		A11, A15	B18, B15, A25, A24, C7, B16, P. laurifolia	B11, B5, B19, A10, C10, B20, C6, C25, B12, C11, A12, B17, B8, B25, B13

Traits	Drought resistance coefficient
Plant height growth (cm)	0.27±0.11
Growth of ground diameter (mm)	0.24±0.07
Net photosynthetic rate (μmol·m^-2·s^-1)	0.19±0.09
Intercellular CO₂ concentration (μmol·mol^-1)	0.52±0.12
Stomatal conductance (mol·m^-2·s^-1)	0.07±0.04
Transpiration rate (mmol·m^-2·s^-1)	0.12±0.05
Stomatal limitation	2.43±0.29
Water use efficiency	1.61±0.12
Chl a (mg·g^-1)	1.00±0.02
Chl b (mg·g^-1)	1.00±0.04
Chl a/b	0.99±0.03
Leaf area (cm²)	0.80±0.07
Relative water content of leaves (%)	0.84±0.07
Catalase (U·mg^-1)	1.23±0.36
Malondialdehyde (nmol·g^-1)	3.24±0.76
Peroxidase (U·g^-1)	0.98±0.14
Proline (μg·g^-1)	1.79±0.23
Superoxide dismutase (U·g^-1)	1.72±0.22

苦杨×小叶杨杂交F₁代苗期抗旱性比较研究

Comparative Study on the Drought Resistance of Young Seedling from Populus laurifolia × P. simonii F₁ Progeny

RichHTML

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

图/表 16

参考文献 26

相关文章 15

编辑推荐

Metrics

本文评价

	H	D	P_n	G_a	C_i	T_r	L_s	WUE	Chl a
Plant height growth (H)	1	0.569**	0.626**	0.658**	0.651**	0.633**	-0.694**	0.635**	0.529**
Growth of ground diameter (D)		1	0.826**	0.785**	0.799**	0.816**	-0.701**	0.786**	0.581**
Net photosynthetic rate (P_n)			1	0.980**	0.908**	0.998**	-0.782**	0.893**	0.726**
Stomatal conductance (G_a)				1	0.845**	0.987**	-0.786**	0.839**	0.690**
Intercellular CO₂concentration (C_i)					1	0.896**	-0.838**	0.946**	0.884**
Transpiration rate (T_r)						1	-0.785**	0.871**	0.722**
Stomatal limitation (L_s)							1	-0.847**	-0.809**
Water use efficiency (WUE)								1	0.821**
Chl a									1
Chl b
Chl a/b
Leaf area (LA)
Relative water content of leaves (LRWC)
Catalase (CAT)
Malondialdehyde (MDA)
Peroxidase (POD)
Proline (Pro)
Superoxide dismutase (SOD)
	Chl b	Chl a/b	LA	LRWC	CAT	MDA	POD	Pro	SOD
Plant height growth (H)	0.540**	-0.415*	0.696**	0.569**	0.509**	-0.318	0.538**	0.721**	0.481**
Growth of ground diameter (D)	0.681**	-0.512**	0.762**	0.619**	0.650**	-0.572**	0.715**	0.722**	0.422*
Net photosynthetic rate (P_n)	0.847**	-0.642**	0.883**	0.686**	0.698**	-0.783**	0.785**	0.838**	0.373*
Stomatal conductance (G_a)	0.824**	-0.637**	0.846**	0.643**	0.640**	-0.725**	0.741**	0.807**	0.337
Intercellular CO₂concentration (C_i)	0.915**	-0.645**	0.917**	0.851**	0.824**	-0.802**	0.873**	0.899**	0.645**
Transpiration rate (T_r)	0.845**	-0.643**	0.875**	0.683**	0.695**	-0.764**	0.788**	0.833**	0.369*
Stomatal limitation (L_s)	-0.820**	0.563**	-0.917**	-0.815**	-0.708**	0.714**	-0.739**	-0.778**	-0.716**
Water use efficiency (WUE)	0.868**	-0.593**	0.940**	0.793**	0.733**	-0.847**	0.776**	0.871**	0.605**
Chl a	0.964**	-0.714**	0.815**	0.881**	0.833**	-0.716**	0.830**	0.783**	0.741**
Chl b	1	-0.761*	0.853**	0.881**	0.820**	-0.768**	0.861**	0.843**	0.637**
Chl a/b		1	-0.566**	-0.640**	-0.627**	0.460*	-0.577**	-0.588**	-0.378*
Leaf area (LA)			1	0.834**	0.760**	-0.768**	0.797**	0.839**	0.582**
Relative water content of leaves (LRWC)				1	0.867**	-0.665**	0.887**	0.751**	0.775**
Catalase (CAT)					1	-0.622**	0.837**	0.714**	0.677**
Malondialdehyde (MDA)						1	-0.688**	-0.665**	-0.514*
Peroxidase (POD)							1	0.810**	0.678**
Proline (Pro)								1	0.641**
Superoxide dismutase (SOD)									1

Principal component	PC1	PC2	PC3
Eigenvalue	13.58	1.30	0.81
Contribution rate	75.45%	7.24%	4.52%
Weight	86.52%	8.30%	5.19%
Plant height growth	0.687	-0.111	0.628
Growth of ground diameter	0.806	-0.279	0.104
Net photosynthetic rate	0.922	-0.369	-0.073
Stomatal conductance	0.889	-0.408	-0.025
Intercellular CO₂ concentration	0.975	-0.013	-0.012
Transpiration rate	0.917	-0.371	-0.064
Stomatal limitation	-0.899	-0.070	-0.190
Water use efficiency	0.945	-0.088	0.015
Chl a	0.904	0.309	-0.149
Chl b	0.948	0.101	-0.200
Chl a/b	-0.701	-0.021	0.337
Leaf area	0.944	-0.074	0.109
Relative water content of leaves	0.890	0.366	-0.026
Catalase	0.848	0.276	-0.097
Malondialdehyde	-0.801	0.054	0.288
Peroxidase	0.895	0.160	-0.056
Proline	0.906	-0.034	0.169
Superoxide dismutase	0.672	0.638	0.224

Individual	D value	Individual	D value
A11	0.91	B19	0.61
A15	0.90	C10	0.60
B18	0.87	B20	0.59
B15	0.77	C25	0.52
Populus laurifolia	0.66	C6	0.51
A24	0.64	B12	0.49
A25	0.64	A12	0.38
B16	0.63	C11	0.38
A10	0.63	B17	0.17
B11	0.63	B8	0.12
B5	0.62	B25	0.12
C7	0.61	B13	0.06

[1]	黄欢, 张佳丽, 杨雪, 陈丽玉, 岳琳, 刘宝辉, 杨慧. 大豆顶芽和腋芽酵母双杂交cDNA文库构建及SOC1a互作蛋白筛选[J]. 植物学报, 2026, 61(3): 386-401.
[2]	郑俊妮, 尚袁凌博, 罗堯, 魏营, 高志伟, 周宗泽, 廖凌娟, 杨道德. 地方重点保护野生动物名录调整方法探究: 以湖南省陆生脊椎动物为例[J]. 生物多样性, 2025, 33(8): 25055-.
[3]	徐进博, 崔雅倩, 王渊, 王伟波, 刘锋, 王广龙, 扈晶晶, 普布顿珠, 边巴多吉, 旦增, 胡开, 王小川, 宋刚, 吕永磊, 温知新. 西藏雅鲁藏布大峡谷国家级自然保护区内白颊猕猴的栖息地适宜性评价[J]. 生物多样性, 2025, 33(7): 24493-.
[4]	郭宇荣, 刘红, 王振华, 田岗, 刘鑫, 郭杰, 李春勇, 李会霞. 长杂谷系列谷子杂交种产量优势及其生理机制[J]. 植物学报, 2025, 60(6): 931-943.
[5]	王文. 同倍杂交物种形成演化出令人惊奇的创新性状[J]. 植物学报, 2025, 60(6): 859-862.
[6]	张琨, 钱敏, 汪阳, 李志华, 孔令娜, 李明洋, 马瑾煜, 努尔艾合麦提•玉苏普, 陈乙一, 成沂芮, 张焕仕, 覃凤飞, 渠晖. 紫花苜蓿耐阴性综合评价及其鉴定指标的筛选[J]. 植物生态学报, 2025, 49(5): 773-787.
[7]	贾盖亚, 张娜, 李宏伟, 李滨, 李振声, 孔照胜, 郑琪. 抗叶锈病小偃麦代换系WTS135的遗传学分析与分子标记开发[J]. 植物学报, 2025, 60(5): 804-815.
[8]	赵凌, 管菊, 梁文化, 张勇, 路凯, 赵春芳, 李余生, 张亚东. 基于高密度Bin图谱的水稻苗期耐热性QTL定位[J]. 植物学报, 2025, 60(3): 342-353.
[9]	尚华丹, 张楚晴, 王梅, 裴文娅, 李国宏, 王鸿斌. 中国杨树害虫物种多样性及其地理分布[J]. 生物多样性, 2025, 33(2): 24370-.
[10]	范惠玲, 路妍, 金文海, 王慧, 彭小星, 武学霞, 刘玉皎. 基于根系表型性状的蚕豆耐盐碱性鉴定与综合评价(长英文摘要)[J]. 植物学报, 2025, 60(2): 204-217.
[11]	吴昱萱, 王平, 胡晓生, 丁一, 彭甜恬, 植秋滢, 巴德木其其格, 李文杰, 关潇, 李俊生. 呼伦贝尔草地退化现状评估与植被特征变化[J]. 生物多样性, 2025, 33(2): 24118-.
[12]	张舒欣, 贾紫璇, 方涛, 刘一凡, 赵微, 王荣, 昌海超, 罗芳丽, 朱耀军, 于飞海. 植物抗逆能力评价方法研究进展[J]. 生物多样性, 2025, 33(2): 24168-.
[13]	田彤彤, 尚博, 徐彦森, 袁相洋, 刘硕, 冯兆忠. 臭氧浓度升高和氮添加对杨树不同叶位和生长时期光合特性的影响[J]. 植物生态学报, 2025, 49(11): 1919-1933.
[14]	顾燚芸, 薛嘉祈, 高金会, 谢心仪, 韦铭, 雷进宇, 闻丞. 一种基于公众科学数据的区域性鸟类多样性评价方法[J]. 生物多样性, 2024, 32(7): 24080-.
[15]	王子阳, 刘升学, 杨志蕊, 秦峰. 玉米抗旱性的遗传解析[J]. 植物学报, 2024, 59(6): 883-902.