大麦抗叶锈病慢锈性鉴定技术及抗性评价方法

doi:10.11983/CBB20074

植物学报 ›› 2020, Vol. 55 ›› Issue (5): 573-576.DOI: 10.11983/CBB20074

大麦抗叶锈病慢锈性鉴定技术及抗性评价方法

车明哲¹, 王亚军³, 马创新¹^,², 漆小泉¹^,²^,^*()

¹中国科学院植物研究所, 植物分子生理学重点实验室, 北京 100093
²中国科学院大学, 北京 100049
³阿卜杜拉国王科技大学生物与环境科学与工程系, 沙漠农业中心, 图沃 23955

收稿日期:2020-05-08 接受日期:2020-07-21 出版日期:2020-09-01 发布日期:2020-09-03
通讯作者: 漆小泉
作者简介:*E-mail: xqi@ibcas.ac.cn
基金资助:
国家自然科学基金(31471756)

Methods for Identification and Resistance Evaluation of Barley Slow Rusting to Leaf Rust

Mingzhe Che¹, Yajun Wang³, Chuangxin Ma¹^,², Xiaoquan Qi¹^,²^,^*()

¹Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
²University of Chinese Academy of Sciences, Beijing 100049, China
³Center for Desert Agriculture, Biological and Environmental Science & Engineering Division, King Abdullah University of Science and Technology, Thuwal 23955, Saudi Arabia

Received:2020-05-08 Accepted:2020-07-21 Online:2020-09-01 Published:2020-09-03
Contact: Xiaoquan Qi

摘要/Abstract

摘要：

成功的病原菌接种和准确的病情调查是大麦(Hordeum vulgare)抗病研究的基础。该文总结了大麦抗叶锈病慢锈性鉴定实验中几种常用的喷雾和涂抹接种方法, 以及主要的抗性评价指标, 并对操作过程中的一些注意事项进行了说明。

关键词: 大麦, 叶锈病, 慢锈性, 接种, 病情调查

Abstract:

Successful pathogen inoculation and following accurate rating are the basis of barley disease resistance studies. Here, we summarized selected barley leaf rust inoculation methods including spraying and smearing, and two evaluation indexes which are widely used in barley slow rusting evaluation. Key issues worthy affecting the assays were also discussed.

Key words: barley, leaf rust, slow rusting, inoculation, rating

车明哲, 王亚军, 马创新, 漆小泉. 大麦抗叶锈病慢锈性鉴定技术及抗性评价方法. 植物学报, 2020, 55(5): 573-576.

Mingzhe Che, Yajun Wang, Chuangxin Ma, Xiaoquan Qi. Methods for Identification and Resistance Evaluation of Barley Slow Rusting to Leaf Rust. Chinese Bulletin of Botany, 2020, 55(5): 573-576.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB20074

https://www.chinbullbotany.com/CN/Y2020/V55/I5/573

图/表 4

图1 大麦繁菌苗保湿效果图(A)和接种后14天产孢效果图(B)

Figure 1 Representative images of barley plants just after spray inoculation and moisture treatment (A) and plants 14 days later when unediniospores became apparent (B)

图2 大麦苗期抗性鉴定育苗示意图(A)及接种塔(B)

Figure 2 Images displaying barley seedlings in the process of inoculation preparation (A) and the settling tower instrument for inoculation (B)

图3 不同大麦品种叶锈菌侵染统计结果 (A) 相对潜伏期(RLP50); (B) 相对侵染频率(RIF)。***P<0.001

Figure 3 Statistical results of leaf rust inoculation in different barley cultivars (A) Measurements of the relative latency period (RLP50); (B) Measurements of the relative infection frequency (RIF). ***P<0.001

图4 大棚鉴定苗圃(A)和发病大麦叶片(B) (B) Bar=1 cm

Figure 4 A nursery in a greenhouse (A) and infected barley leaves (B) (B) Bar=1 cm

参考文献 8

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大麦抗叶锈病慢锈性鉴定技术及抗性评价方法

Methods for Identification and Resistance Evaluation of Barley Slow Rusting to Leaf Rust

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