As one of the dual wings of innovative development, science popularization is a crucial means of enhancing national scientific literacy. Universities, as the main platforms for disseminating knowledge and nurturing talent, also bear the social responsibility of promoting science popularization. In biology, conducting science education and guiding students to extract biological knowledge from their courses to create popular science content is of great significance in advancing public science literacy. This article takes a university botany course as an example to illustrate how botany courses can integrate popular science education through course knowledge, practical activities, research outcomes, and real-life applications. By employing the “2W1H” approach (What, Why, and How), the paper guides students in creating popular science. This not only cultivates students’ comprehensive and innovative abilities but also disseminates biological knowledge, offering a valuable reference for the cultivation of popular science talents in the context of promoting science literacy for all.